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UniProtKB/Swiss-Prot entry Q04697


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GSF2_YEAST
Primary accession number Q04697
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on November 1, 1997 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 54)
Name and origin of the protein
Protein name Glucose-signaling factor 2
Synonym Extracellular mutant protein 6
Gene name
Name: GSF2
Synonyms: ECM6, EFF2
OrderedLocusNames: YML048W
ORFNames: YM9827.04
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169872 [NCBI, ExPASy, EBI, Israel, Japan]
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
Nature 387:90-93(1997).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[3]
IDENTIFICATION.
PubMed=9335584 [NCBI, ExPASy, EBI, Israel, Japan]
Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H.;
"Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae.";
Genetics 147:435-450(1997).
[4]
FUNCTION.
PubMed=9335593 [NCBI, ExPASy, EBI, Israel, Japan]
Sherwood P.W., Carlson M.;
"Mutations in GSF1 and GSF2 alter glucose signaling in Saccharomyces cerevisiae.";
Genetics 147:557-566(1997).
[5]
FUNCTION, AND SUBCELLULAR LOCATION.
DOI=10.1073/pnas.96.13.7415; PubMed=10377429 [NCBI, ExPASy, EBI, Israel, Japan]
Sherwood P.W., Carlson M.;
"Efficient export of the glucose transporter Hxt1p from the endoplasmic reticulum requires Gsf2p.";
Proc. Natl. Acad. Sci. U.S.A. 96:7415-7420(1999).
[6]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02026; PubMed=14562095 [NCBI, ExPASy, EBI, Israel, Japan]
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.;
"Global analysis of protein localization in budding yeast.";
Nature 425:686-691(2003).
[7]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z47816; CAA87826.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY692925; AAT92944.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S50944; S50944.
RefSeq NP_013664.1; -.
3D structure databases
ModBase Q04697.
Protein-protein interaction databases
DIP DIP:4541N; -.
IntAct Q04697; -.
Organism-specific databases
CYGD YML048w; -.
SGD S000004511; GSF2.
Yeast-GFP YML048W.
Gene expression databases
ArrayExpress Q04697; -.
GermOnline YML048W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0030176; Cellular component: integral to endoplasmic reticulum membrane (inferred from direct assay from SGD).
GO:0005741; Cellular component: mitochondrial outer membrane (inferred from direct assay from SGD).
GO:0051082; Molecular function: unfolded protein binding (inferred from mutant phenotype from SGD).
GO:0006457; Biological process: protein folding (inferred from mutant phenotype from SGD).
GO:0034394; Biological process: protein localization at cell surface (inferred from genetic interaction from SGD).
QuickGo view.
Family and domain databases
BLOCKS Q04697.
Proteomic databases
PeptideAtlas Q04697; -.
Genome annotation databases
Ensembl YML048W; Saccharomyces cerevisiae. [Contig view]
GeneID 854957; -.
GenomeReviews Z71257_GR; YML048W.
KEGG sce:YML048W; -.
NMPDR fig|4932.3.peg.4703; -.
Phylogenomic databases
HOGENOM Q04697; -.
Other
LinkHub Q04697; -.
ProtoNet Q04697.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Coiled coil; Complete proteome; Endoplasmic reticulum; Glycoprotein; Membrane; Signal-anchor; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   403  403     Glucose-signaling factor 2. PRO_0000083865
TOPO_DOM   1   177  177     Lumenal (Potential). 
TRANSMEM   178   198  21     Signal-anchor for type II membrane protein (Potential). 
TOPO_DOM   199   403  205     Cytoplasmic (Potential). 
COILED   330   388  59     Potential. 
CARBOHYD   89    89        N-linked (GlcNAc...) (Potential). 
CARBOHYD   173   173        N-linked (GlcNAc...) (Potential). 
Sequence information
Length: 403 AA [This is the length of the unprocessed precursor] Molecular weight: 45870 Da [This is the MW of the unprocessed precursor] CRC64: 096E07AFFA711ECB [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEIYIRLNAD VEHDYAFQVS NEDTINNKIK KIFPSKTGLA DLMVLRPSIF HEKEPVKFYK 

        70         80         90        100        110        120 
SIHPGYLSEG GCLMFHYEAD NEENLEELND SKPLIDQLWP GQLVVPEWKL SKKNIWVYTI 

       130        140        150        160        170        180 
IMLAWLYTDL PDAISPTPGI CLTNQLSRLL IPVAKHMDLP EIAAKLEQEV QANYSSLVAQ 

       190        200        210        220        230        240 
WLFFVMHIFK VGIITLFLKL GIANPISFNP YKLWSLRDLT SPSANGAKNS GGNNNTTDLK 

       250        260        270        280        290        300 
TRLRSLGWIG AKRATYDDYQ TNYYNYVIDK MGGAVAAYRA GAIRKAAAPG IQLVAGEGFQ 

       310        320        330        340        350        360 
SPLEDRFTAS TFTAIKTERK FILSEEYFVE LENNLKKILE EYDGDIGKMN AEIRRFRRFG 

       370        380        390        400 
IYEPDEKLAS LVKLRREIAD EKEKASNNDA TFGIKKNDLK KSN 

Q04697 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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