ID   L_SV5WR                 Reviewed;        2255 AA.
AC   Q03396;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-NOV-2008, entry version 48.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Simian virus 5 (strain 21004-WR) (SV5).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Paramyxoviridae; Paramyxovirinae; Rubulavirus.
OX   NCBI_TaxID=33730;
OH   NCBI_TaxID=9615; Canis familiaris (Dog).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9541; Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey).
OH   NCBI_TaxID=9544; Macaca mulatta (Rhesus macaque).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=92341049; PubMed=1321873;
RA   Higuchi Y., Miyahara Y., Kawano M., Tsurudome M., Matsumura H.,
RA   Kusagawa S., Komada H., Nishio M., Ito Y.;
RT   "Sequence analysis of the large (L) protein of simian virus 5.";
RL   J. Gen. Virol. 73:1005-1010(1992).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently the subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The transcriptase
CC       stutters on a specific sequence, resulting on a cotranscriptional
CC       editing of the phosphoprotein (P) mRNA. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the paramyxoviruses L protein family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; D13868; BAA02981.1; -; Genomic_RNA.
DR   PIR; JQ1532; JQ1532.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR016269; RNA-dir_RNA_pol_paramyxovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF000830; RNA_pol_ParamyxoV; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Methyltransferase; mRNA capping;
KW   mRNA processing; Multifunctional enzyme; Nucleotide-binding;
KW   Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase;
KW   S-adenosyl-L-methionine; Transferase; Virion.
FT   CHAIN         1   2255       Large structural protein.
FT                                /FTId=PRO_0000142743.
FT   DOMAIN      656    840       RdRp catalytic.
FT   NP_BIND    1805   1814       ATP (Potential).
SQ   SEQUENCE   2255 AA;  255941 MW;  2977A0C951C38A72 CRC64;
     MAGSREILLP EVHLNSPIVK HKLYYYILLG NLPNEIDLDD LGPLHNQNWN QIAHEESNLA
     QRLVNVRNFL ITHIPDLRKG HWQEYVNVIL WPRILKLLPD FKINDQLPLL KNWDKLVKES
     CSVINAGTSQ CIQNLSYGLT GRGNLFTRSR ELSGDRRDID LKTVVAAWHD SDWKRISDFW
     IMIKFQMRQL IVRQTDHNDP DLITYIENRE GIIIITPELV ALFNTENHTL TYMTFEIVLM
     VSDMYEGRHN ILSLCTVSTY LNPLKKRITY LLSLVDNLAF QIGDAVYNII ALLESFVYAQ
     LQMSDPIPEL RGQFHAFVCS EILDALRGTN SFTQDELRTV TTNLISPFQD LTPDLTAELL
     CIMRLWGHPM LTASQAAGKV RESMCAGKVL DFPTIMKTLA FFHTILINGY RRKHHGVWPP
     LNLPGNASKG LTELMNDNTE ISYEFTLKHW KEVSLIKFKK CFDADAGEEL SIFMKDKAIS
     APKQDWMSVF RRSLIKQRHQ HHQVPLPNPF NRRLLLNFLG DDKFDPSVEL QYVTSGEYLH
     DDTFCASYSL KEKEIKPDGR IFAKLTKRMR SCQVIAESLL ANHAGLLMKE NGVVMNQLSL
     TKSLLTMSQI GIISEKARKS TRDNINQPGF QNIQRNKSHH SKQVNQRDPS DDFELAASFL
     TTDLKKYCLQ WRYQTIIPFA QSLNRMYGYP HLFEWIHLRL MRSTLYVGDP FNPPADTSQF
     DLDKVINGDI FIVSPRGGIE GLCQKAWTMI SIAVIILSAT ESGTRVMSMV QGDNQAIAVT
     TRVPRSLPTL EKKTIAFRSC NLFFERLKCN NFGLGHHLKE QETIISSHFF VYSKRIFYQG
     RILTQALKNA SKLCLTADVL GECTQSSCSN LATTVMRLTE NGVEKDICFY LNIYMTIKQL
     SYDIIFPQVS IPGDQITLEY INNPHLVSRL ALLPSQLGGL NYLSCSRLFN RNIGDPVVSA
     VADLKRLIKS GCMDYWILYN LLGRKPGNGS WATLAADPYS INIEYQYPPT TALKRHTQQA
     LMELSTNPML RGIFSDNAQA EENNLARFLL DREVIFPRVA HIIIEQTSVG RRKQIQGYLD
     STRSIMRKSL EIKPLSNRKL NEILDYNINY LAYNLALLKN AIEPPTYLKA MTLETCSIDI
     ARNLRKLSWA PLLGGRNLEG LETPDPIEIT AGALIVGSGY CEQCAAGDNR FTWFFLPSGI
     EIGGDPRDNP PIRVPYIGSR TDERRVASMA YIRGASSSLK AVLRLAGVYI WAFGDTLENW
     IDALDLSHTR VNITLEQLQS LTPLPTSANL THRLDDGTTT LKFTPASSYT FSSFTHISND
     EQYLTINDKT ADSNIIYQQL MITGLGILET WNNPPVNRTF EESTLHLHTG ASCCVRPVDS
     CIISEALTVK PHITVPYSNK FVFDEDPLSE YETAKLESLS FQAQLGNIDA VDMTGKLTLL
     SQFTARQIIN AITGLDESVS LTNDAIVASD YVSNWISECM YTKLDELFMY CGWELLLELS
     YQMYYLRVVG WSNIVDYSYM ILRRIPGAAL NNLASTLSHP KLFRRAINLD IVAPLNAPHF
     ASLDYIKMSV DAILWGCKRV INVLSNGGDL ELVVTSEDSL ILSDRSMNLI ARKLTLLSLI
     HHNGLELPKI KGFSPDEKCF ALTEFLRKVV NSGLSSIENL SNFMYNVENP RLAAFASNNY
     YLTRKLLNSI RDTESGQVAV TSYYESLEYI DSLKLTPHVP GTSCIEDDSL CTNDYIIWII
     ESNANLEKYP IPNSPEDDSN FHNFKLNAPS HHTLRPLGLS STAWYKGISC CRYLERLKLP
     QGDHLYIAEG SGASMTIIEY LFPGRKIYYN SLFSSGDNPP QRNYAPMPTQ FIESVPYKLW
     QAHTDQYPEI FEDFIPLWNG NATMTDIGMT ACVEFIINRV GPRTCSLVHV DLESSASLNQ
     QCLSKPIINA IITATTVLCP HGVLILKYSW LPFTRFSTLI TFLWCYFERI TVLRSTYSDP
     ANHEVYLICI LANNFAFQTV SQATGMAMTL TDQGFTLISP ERINQYWDGH LKQERIVAEA
     IDKVVLGENA LFNSSDNELI LKCGGTPNAR NLIDIEPVAT FIEFEQLICT MLTTHLKEII
     DITRSGTQDY ESLLLTPYNL GLLGKISTIV RLLTERILNH TIRNWLILPP SLRMIVKQDL
     EFGIFRITSI LNSDRFLKLS PNRKYLIAQL TAGYIRKLIE GDCNIDLTRP IQKQIWKALG
     CVVYCHDPMD QRESTEFIDI NINEEIDRGI DGEEI
//