RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Simian virus 5 (strain 21004-WR) (SV5)

[TaxID: 33730]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Rubulavirus.

Virus hosts

Canis familiaris (Dog) [TaxID: 9615]
Homo sapiens (Human) [TaxID: 9606]
Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey) [TaxID: 9541]
Macaca mulatta (Rhesus macaque) [TaxID: 9544]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. PubMed=1321873 [NCBI, ExPASy, EBI, Israel, Japan] Higuchi Y., Miyahara Y., Kawano M., Tsurudome M., Matsumura H., Kusagawa S., Komada H., Nishio M., Ito Y.; "Sequence analysis of the large (L) protein of simian virus 5."; J. Gen. Virol. 73:1005-1010(1992).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D13868; BAA02981.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JQ1532; JQ1532.

3D structure databases

ModBase

Q03396.

Family and domain databases

InterPro

IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000830; RNA_pol_ParamyxoV; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2255`	`2255`	`Large structural protein.`	`PRO_0000142743`
`DOMAIN`	`656 840`	`185`	`RdRp catalytic.`
`NP_BIND`	`1805 1814`	`10`	`ATP (Potential).`

Sequence information

Length: 2255 AA [This is the length of the unprocessed precursor]

Molecular weight: 255941 Da [This is the MW of the unprocessed precursor]

CRC64: 2977A0C951C38A72 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAGSREILLP EVHLNSPIVK HKLYYYILLG NLPNEIDLDD LGPLHNQNWN QIAHEESNLA 

        70         80         90        100        110        120 
QRLVNVRNFL ITHIPDLRKG HWQEYVNVIL WPRILKLLPD FKINDQLPLL KNWDKLVKES 

       130        140        150        160        170        180 
CSVINAGTSQ CIQNLSYGLT GRGNLFTRSR ELSGDRRDID LKTVVAAWHD SDWKRISDFW 

       190        200        210        220        230        240 
IMIKFQMRQL IVRQTDHNDP DLITYIENRE GIIIITPELV ALFNTENHTL TYMTFEIVLM 

       250        260        270        280        290        300 
VSDMYEGRHN ILSLCTVSTY LNPLKKRITY LLSLVDNLAF QIGDAVYNII ALLESFVYAQ 

       310        320        330        340        350        360 
LQMSDPIPEL RGQFHAFVCS EILDALRGTN SFTQDELRTV TTNLISPFQD LTPDLTAELL 

       370        380        390        400        410        420 
CIMRLWGHPM LTASQAAGKV RESMCAGKVL DFPTIMKTLA FFHTILINGY RRKHHGVWPP 

       430        440        450        460        470        480 
LNLPGNASKG LTELMNDNTE ISYEFTLKHW KEVSLIKFKK CFDADAGEEL SIFMKDKAIS 

       490        500        510        520        530        540 
APKQDWMSVF RRSLIKQRHQ HHQVPLPNPF NRRLLLNFLG DDKFDPSVEL QYVTSGEYLH 

       550        560        570        580        590        600 
DDTFCASYSL KEKEIKPDGR IFAKLTKRMR SCQVIAESLL ANHAGLLMKE NGVVMNQLSL 

       610        620        630        640        650        660 
TKSLLTMSQI GIISEKARKS TRDNINQPGF QNIQRNKSHH SKQVNQRDPS DDFELAASFL 

       670        680        690        700        710        720 
TTDLKKYCLQ WRYQTIIPFA QSLNRMYGYP HLFEWIHLRL MRSTLYVGDP FNPPADTSQF 

       730        740        750        760        770        780 
DLDKVINGDI FIVSPRGGIE GLCQKAWTMI SIAVIILSAT ESGTRVMSMV QGDNQAIAVT 

       790        800        810        820        830        840 
TRVPRSLPTL EKKTIAFRSC NLFFERLKCN NFGLGHHLKE QETIISSHFF VYSKRIFYQG 

       850        860        870        880        890        900 
RILTQALKNA SKLCLTADVL GECTQSSCSN LATTVMRLTE NGVEKDICFY LNIYMTIKQL 

       910        920        930        940        950        960 
SYDIIFPQVS IPGDQITLEY INNPHLVSRL ALLPSQLGGL NYLSCSRLFN RNIGDPVVSA 

       970        980        990       1000       1010       1020 
VADLKRLIKS GCMDYWILYN LLGRKPGNGS WATLAADPYS INIEYQYPPT TALKRHTQQA 

      1030       1040       1050       1060       1070       1080 
LMELSTNPML RGIFSDNAQA EENNLARFLL DREVIFPRVA HIIIEQTSVG RRKQIQGYLD 

      1090       1100       1110       1120       1130       1140 
STRSIMRKSL EIKPLSNRKL NEILDYNINY LAYNLALLKN AIEPPTYLKA MTLETCSIDI 

      1150       1160       1170       1180       1190       1200 
ARNLRKLSWA PLLGGRNLEG LETPDPIEIT AGALIVGSGY CEQCAAGDNR FTWFFLPSGI 

      1210       1220       1230       1240       1250       1260 
EIGGDPRDNP PIRVPYIGSR TDERRVASMA YIRGASSSLK AVLRLAGVYI WAFGDTLENW 

      1270       1280       1290       1300       1310       1320 
IDALDLSHTR VNITLEQLQS LTPLPTSANL THRLDDGTTT LKFTPASSYT FSSFTHISND 

      1330       1340       1350       1360       1370       1380 
EQYLTINDKT ADSNIIYQQL MITGLGILET WNNPPVNRTF EESTLHLHTG ASCCVRPVDS 

      1390       1400       1410       1420       1430       1440 
CIISEALTVK PHITVPYSNK FVFDEDPLSE YETAKLESLS FQAQLGNIDA VDMTGKLTLL 

      1450       1460       1470       1480       1490       1500 
SQFTARQIIN AITGLDESVS LTNDAIVASD YVSNWISECM YTKLDELFMY CGWELLLELS 

      1510       1520       1530       1540       1550       1560 
YQMYYLRVVG WSNIVDYSYM ILRRIPGAAL NNLASTLSHP KLFRRAINLD IVAPLNAPHF 

      1570       1580       1590       1600       1610       1620 
ASLDYIKMSV DAILWGCKRV INVLSNGGDL ELVVTSEDSL ILSDRSMNLI ARKLTLLSLI 

      1630       1640       1650       1660       1670       1680 
HHNGLELPKI KGFSPDEKCF ALTEFLRKVV NSGLSSIENL SNFMYNVENP RLAAFASNNY 

      1690       1700       1710       1720       1730       1740 
YLTRKLLNSI RDTESGQVAV TSYYESLEYI DSLKLTPHVP GTSCIEDDSL CTNDYIIWII 

      1750       1760       1770       1780       1790       1800 
ESNANLEKYP IPNSPEDDSN FHNFKLNAPS HHTLRPLGLS STAWYKGISC CRYLERLKLP 

      1810       1820       1830       1840       1850       1860 
QGDHLYIAEG SGASMTIIEY LFPGRKIYYN SLFSSGDNPP QRNYAPMPTQ FIESVPYKLW 

      1870       1880       1890       1900       1910       1920 
QAHTDQYPEI FEDFIPLWNG NATMTDIGMT ACVEFIINRV GPRTCSLVHV DLESSASLNQ 

      1930       1940       1950       1960       1970       1980 
QCLSKPIINA IITATTVLCP HGVLILKYSW LPFTRFSTLI TFLWCYFERI TVLRSTYSDP 

      1990       2000       2010       2020       2030       2040 
ANHEVYLICI LANNFAFQTV SQATGMAMTL TDQGFTLISP ERINQYWDGH LKQERIVAEA 

      2050       2060       2070       2080       2090       2100 
IDKVVLGENA LFNSSDNELI LKCGGTPNAR NLIDIEPVAT FIEFEQLICT MLTTHLKEII 

      2110       2120       2130       2140       2150       2160 
DITRSGTQDY ESLLLTPYNL GLLGKISTIV RLLTERILNH TIRNWLILPP SLRMIVKQDL 

      2170       2180       2190       2200       2210       2220 
EFGIFRITSI LNSDRFLKLS PNRKYLIAQL TAGYIRKLIE GDCNIDLTRP IQKQIWKALG 

      2230       2240       2250 
CVVYCHDPMD QRESTEFIDI NINEEIDRGI DGEEI

Q03396 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools