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UniProtKB/Swiss-Prot entry Q03216

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name K6PF2_KLULA
Primary accession number Q03216
Secondary accession number Q6CL26
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on September 27, 2004 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 62)
Name and origin of the protein
Protein name 6-phosphofructokinase subunit beta
Synonyms EC 2.7.1.11
Phosphofructokinase 2
Phosphohexokinase
6PF-1-K subunit beta
Gene name
Name: PFK2
OrderedLocusNames: KLLA0F06248g
From
Kluyveromyces lactis (Yeast) (Candida sphaerica) [TaxID: 28985] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
PubMed=8326866 [NCBI, ExPASy, EBI, Israel, Japan]
Heinisch J.J., Kirchrath L., Liesen T., Vogelsang K., Hollenberg C.P.;
"Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis.";
Mol. Microbiol. 8:559-570(1993).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37;
DOI=10.1038/nature02579; PubMed=15229592 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S., Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.;
"Genome evolution in yeasts.";
Nature 430:35-44(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z17316; CAA78964.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CR382126; CAG98071.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S32903; S32903.
RefSeq XP_455363.1; -.
3D structure databases
HSSP P00512; 3PFK. [HSSP ENTRY / PDB]
ModBase Q03216.
Ontologies
GO
GO:0005945; Cellular component: 6-phosphofructokinase complex (inferred from electronic annotation from InterPro).
GO:0003872; Molecular function: 6-phosphofructokinase activity (inferred from electronic annotation from InterPro).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006096; Biological process: glycolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR009161; 6-phosphofructokinase_euk.
IPR015912; Phosphofructokinase_CS.
IPR000023; Ppfruckinase.
Graphical view of domain structure.
PANTHER PTHR13697; Ppfruckinase; 1.
Pfam PF00365; PFK; 2.
Pfam graphical view of domain structure.
PIRSF PIRSF000533; ATP_PFK_euk; 1.
PRINTS PR00476; PHFRCTKINASE.
ProDom PD000707; Ppfruckinase; 2.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR02478; 6PF1K_euk; 1.
PROSITE PS00433; PHOSPHOFRUCTOKINASE; 2.
ProtoNet Q03216.
Genome annotation databases
GeneID 2894998; -.
KEGG kla:KLLA0F06248g; -.
Phylogenomic databases
HOGENOM Q03216; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Allosteric enzyme; ATP-binding; Complete proteome; Cytoplasm; Glycolysis; Kinase; Nucleotide-binding; Repeat; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   938  938     6-phosphofructokinase subunit beta. PRO_0000112041
NP_BIND   195   199  5     ATP (By similarity). 
NP_BIND   354   358  5     ATP (By similarity). 
NP_BIND   371   387  17     ATP (By similarity). 
ACT_SITE   327   327        Proton acceptor (By similarity). 
BINDING   362   362        Substrate (By similarity). 
BINDING   454   454        Substrate (By similarity). 
BINDING   460   460        Substrate (By similarity). 
BINDING   463   463        Substrate (By similarity). 
CONFLICT   523   523        Missing (in Ref. 1; CAA78964). 
CONFLICT   554   554        D -> H (in Ref. 1; CAA78964). 
CONFLICT   783   784        FS -> LR (in Ref. 1; CAA78964). 
CONFLICT   927   938        TIADHLVGRKKL -> PLRTICRKEKTYEIKNVSASSKFPLKWMYIIM (in Ref. 1; CAA78964). 
Sequence information
Length: 938 AA [This is the length of the unprocessed precursor] Molecular weight: 102477 Da [This is the MW of the unprocessed precursor] CRC64: CE9C56DD7588C955 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTQSLPLLNG TEAYKLVTTQ GLYDKTVKFY EKYLQLVHDK RVGTLTNSLI TLKLVVDNSF 

        70         80         90        100        110        120 
KPLDVVNDKD WRAIVSSALV FSCTNIQHFR DLAAGETIQA YPNETNPIEI YLKDPNGYII 

       130        140        150        160        170        180 
GITETKNAIS IKPTLPKQSV EASLISSRSS RIDIASSGVS TDSSYPAIPK TAKAQKSIAV 

       190        200        210        220        230        240 
MTSGGDAPGM NANVRAIVRT AIFKGCNAFV VMEGYEGLVK GGPNYIKQVY WETVRNWSCE 

       250        260        270        280        290        300 
GGTNIGTARC KEFREREGRL LGALHLIEAG VDALIVCGGD GSLTGADLFR SEWPSLIREL 

       310        320        330        340        350        360 
LDQGRINKVQ FDRYQHLNIC GTVGSIDNDM STTDATIGAY SALDRICQAI DYIEATANSH 

       370        380        390        400        410        420 
SRAFVVEVMG RNCGWLALLA GISTSADYIL IPEKPASSRE WQDQMCDIIS KHRSRGKRTT 

       430        440        450        460        470        480 
IVIVAEGAIS ADLTPISSKD VHKVLVDRLG LDCRITTLGH VQRGGTAVAY DRILATLQGV 

       490        500        510        520        530        540 
EAVNAVLEST PDTPSPLIAI NENKITRKPL VESVQLTKSV AEAIHSKDFK KAMQLRDSEF 

       550        560        570        580        590        600 
VEHLDNFMAI NSADHIEPKL PEHTHMKIAI VNVGAPAGGM NSAVYSMATY CMSQGHKPYA 

       610        620        630        640        650        660 
IYNGWTGLTR HESVRSLNWK DLLGWQSRGG SEIGTNRHTP EEADIGLIAY YFQKYGFDGI 

       670        680        690        700        710        720 
IIVGGFEAFV SLHQLERARE NYTAFRIPMV LIPATLSNNV PGTEYSLGSD TALNSLMQYC 

       730        740        750        760        770        780 
DIIKQSAAST RGRVFVVDVQ GGNSGYLATH AAVAVGAQVS YVPEEGISLE QLTQDIENLT 

       790        800        810        820        830        840 
ESFSEAEGRG KFGQLILKST NASKVLTPEV LAEVITQEAE GHFDAKCAIP GHVQQGGLPS 

       850        860        870        880        890        900 
PIDRTRGTRF AIRAVGFIES QHKVLAAEAN LDDDDFDFDT PKIIATASVL GVKGSDIVFS 

       910        920        930 
SIRQLYDFET ELNKRTPKTI HWQSTRTIAD HLVGRKKL
Q03216 in FASTA format

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