[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. STRAIN=Sprague-Dawley; TISSUE=Liver; PubMed=8471037 [NCBI, ExPASy, EBI, Israel, Japan] Donaldson I.A., Doyle T.C., Matas N.; "Expression of rat liver ketohexokinase in yeast results in fructose intolerance."; Biochem. J. 291:179-186(1993).
[2]	PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING. PubMed=9799106 [NCBI, ExPASy, EBI, Israel, Japan] Hayward B.E., Bonthron D.T.; "Structure and alternative splicing of the ketohexokinase gene."; Eur. J. Biochem. 257:85-91(1998).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). TISSUE=Kidney; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

CATALYTIC ACTIVITY: ATP + D-fructose = ADP + D-fructose 1-phosphate.
ENZYME REGULATION: Requires potassium. Inhibition by ADP.
PATHWAY: Carbohydrate metabolism; fructose metabolism .
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name C

Isoform ID Q02974-1

This is the isoform sequence displayed in this entry.

Name A

Isoform ID Q02974-2

Features which should be applied to build the isoform sequence: VSP_004670.
TISSUE SPECIFICITY: Most abundant in the liver.
PTM: The N-terminus is blocked.
SIMILARITY: Belongs to the carbohydrate kinase pfkB family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X63658; CAA45198.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Y09339; CAA70520.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Y09338; CAA70518.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Y09338; CAA70519.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC078752; AAH78752.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S32426; S32426.

NP_114061.1; -.

3D structure databases

SMR

Q02974; 5-298.

ModBase

Q02974.

Organism-specific databases

RGD

2966; Khk.

Gene expression databases

ArrayExpress

Q02974; -.

GermOnline

ENSRNOG00000008047; Rattus norvegicus.

Ontologies

GO:0004454;	Molecular function: ketohexokinase activity (inferred from electronic annotation from EC).
GO:0005975;	Biological process: carbohydrate metabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR011611; Carb/pur_kinase.
IPR002173; Carboh/pur_kinase_PfkB_CS.
Graphical view of domain structure.

Pfam

PF00294; PfkB; 2.
Pfam graphical view of domain structure.

PROSITE

PS00583; PFKB_KINASES_1; FALSE_NEG.
PS00584; PFKB_KINASES_2; FALSE_NEG.

ProtoNet

Q02974.

Genome annotation databases

Ensembl

ENSRNOG00000008047; Rattus norvegicus. [Contig view]

GeneID

25659; -.

KEGG

rno:25659; -.

Phylogenomic databases

HOVERGEN

Q02974; -.

Other

NextBio

607557; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Carbohydrate metabolism; Direct protein sequencing; Kinase; Phosphoprotein; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 298`	`298`	`Ketohexokinase.`	`PRO_0000080091`
`MOD_RES`	`169 169`		`Phosphoserine (By similarity).`
`VAR_SEQ`	`72 114`		`LVADFRRRGVDVSQVAWQSQGDTPCSCCIVNNSNGSRT` `IILYD -> VLDDLRRHSVDLRYAVLQTEGSIPTSTVIVNEASGSRT` `ILHAY (in isoform A).`	`VSP_004670`

Sequence information

Length: 298 AA [This is the length of the unprocessed precursor]

Molecular weight: 32749 Da [This is the MW of the unprocessed precursor]

CRC64: F85C787FF047DF70 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEEKQILCVG LVVLDIINVV DKYPEEDTDR RCLSQRWQRG GNASNSCTVL SLLGARCAFM 

        70         80         90        100        110        120 
GSLAHGHVAD FLVADFRRRG VDVSQVAWQS QGDTPCSCCI VNNSNGSRTI ILYDTNLPDV 

       130        140        150        160        170        180 
SAKDFEKVDL TRFKWIHIEG RNASEQVKML QRIEQYNATQ PLQQKVRVSV EIEKPREELF 

       190        200        210        220        230        240 
QLFGYGEVVF VSKDVAKHLG FRSAGEALKG LYSRVKKGAT LICAWAEEGA DALGPDGQLL 

       250        260        270        280        290 
HSDAFPPPRV VDTLGAGDTF NASVIFSLSK GNSMQEALRF GCQVAGKKCG LQGFDGIV

Q02974 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools