[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1). STRAIN=DBA/2; PubMed=1454796 [NCBI, ExPASy, EBI, Israel, Japan] McNeil H.P., Reynolds D.S., Schiller V., Ghildyal N., Gurley D.S., Austen K.F., Stevens R.L.; "Isolation, characterization, and transcription of the gene encoding mouse mast cell protease 7."; Proc. Natl. Acad. Sci. U.S.A. 89:11174-11178(1992).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2). STRAIN=C57BL/6J; DOI=10.1074/jbc.271.5.2851; PubMed=8576265 [NCBI, ExPASy, EBI, Israel, Japan] Hunt J.E., Stevens R.L., Austen K.F., Zhang J., Xia Z., Ghildyal N.; "Natural disruption of the mouse mast cell protease 7 gene in the C57BL/6 mouse."; J. Biol. Chem. 271:2851-2855(1996).

Comments

FUNCTION: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
CATALYTIC ACTIVITY: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing. The alternative splicing event is due to a G to A point mutation at the exon 2/intron 2 splice site and causes loss of protein expression. The alternatively spliced transcript is only found in C57BL/6 mouse.

Name	1
Isoform ID	Q02844-1
This is the isoform sequence displayed in this entry.

Name	2
Synonyms	Truncated
Isoform ID	Q02844-2
Features which should be applied to build the isoform sequence: VSP_005378, VSP_005379.

DEVELOPMENTAL STAGE: Is not expressed in mature serosal or mucosal mast cells and is expressed only transiently at an early stage of in vitro mast cell differentiation.
SIMILARITY: Belongs to the peptidase S1 family. Tryptase subfamily [view classification].
SIMILARITY: Contains 1 peptidase S1 domain [view classification].

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L00653; AAA39992.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00654; AAA39993.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U42405; AAA97874.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U42406; AAA97875.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A47246; A47246.

UniGene

Mm.3301

3D structure databases

HSSP

P15157; 1LTO. [HSSP ENTRY / PDB]

SMR

Q02844; 29-271.

ModBase

Q02844.

Protein family/group databases

MEROPS

S01.026; -.

Organism-specific databases

MGI

MGI:96943; Tpsab1.

Gene expression databases

ArrayExpress

Q02844; -.

CleanEx

MM_TPSAB1; -.

GermOnline

ENSMUSG00000024173; Mus musculus.

Ontologies

GO:0004252;	Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0006508;	Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR001254; Peptidase_S1_S6.
IPR001314; Peptidase_S1A.
Graphical view of domain structure.

Pfam

PF00089; Trypsin; 1.
Pfam graphical view of domain structure.

PRINTS

PR00722; CHYMOTRYPSIN.

SMART

SM00020; Tryp_SPc; 1.
SMART graphical view of domain structure.

PROSITE

PS50240; TRYPSIN_DOM; 1.
PS00134; TRYPSIN_HIS; 1.
PS00135; TRYPSIN_SER; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q02844.

Genome annotation databases

Ensembl

ENSMUSG00000024173; Mus musculus. [Contig view]

Phylogenomic databases

HOGENOM

Q02844; -.

HOVERGEN

Q02844; -.

Other

SOURCE

Tpsab1; Mus musculus.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Glycoprotein; Hydrolase; Protease; Serine protease; Signal; Zymogen.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 18`	`18`	`Potential.`
`PROPEP`	`19 28`	`10`	`Activation peptide (Potential).`	`PRO_0000027494`
`CHAIN`	`29 273`	`245`	`Tryptase.`	`PRO_0000027495`
`DOMAIN`	`29 270`	`242`	`Peptidase S1.`
`ACT_SITE`	`72 72`		`Charge relay system (By similarity).`
`ACT_SITE`	`119 119`		`Charge relay system (By similarity).`
`ACT_SITE`	`222 222`		`Charge relay system (By similarity).`
`CARBOHYD`	`49 49`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`130 130`		`N-linked (GlcNAc...) (Potential).`
`DISULFID`	`57 73`		`By similarity.`
`DISULFID`	`153 228`		`By similarity.`
`DISULFID`	`186 209`		`By similarity.`
`DISULFID`	`218 246`		`By similarity.`
`VAR_SEQ`	`44 46`		`VSL -> GCC (in isoform 2).`	`VSP_005378`
`VAR_SEQ`	`47 273`		`Missing (in isoform 2).`	`VSP_005379`

Sequence information

Length: 273 AA [This is the length of the unprocessed precursor]

Molecular weight: 30337 Da [This is the MW of the unprocessed precursor]

CRC64: 50ECB4765294205E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLKLLLLTLP LLSSLVHAAP GPAMTREGIV GGQEAHGNKW PWQVSLRAND TYWMHFCGGS 

        70         80         90        100        110        120 
LIHPQWVLTA AHCVGPDVAD PNKVRVQLRK QYLYYHDHLM TVSQIITHPD FYIVQDGADI 

       130        140        150        160        170        180 
ALLKLTNPVN ISDYVHPVPL PPASETFPSG TLCWVTGWGN IDNGVNLPPP FPLKEVQVPI 

       190        200        210        220        230        240 
IENHLCDLKY HKGLITGDNV HIVRDDMLCA GNEGHDSCQG DSGGPLVCKV EDTWLQAGVV 

       250        260        270 
SWGEGCAQPN RPGIYTRVTY YLDWIHHYVP KDF

Q02844 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools