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UniProtKB/Swiss-Prot entry Q02401

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LPH_RAT
Primary accession number Q02401
Secondary accession numbers Q63712 Q63719
Integrated into Swiss-Prot on July 1, 1993
Sequence was last modified on May 30, 2000 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 79)
Name and origin of the protein
Protein name Lactase-phlorizin hydrolase [Precursor]
Synonym Lactase-glycosylceramidase
Includes Lactase
     (EC 3.2.1.108)
Phlorizin hydrolase
     (EC 3.2.1.62)
Gene name
Name: Lct
Synonyms: Lph
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Wistar;
TISSUE=Intestine;
DOI=10.1016/0378-1119(91)90286-K; PubMed=1909681 [NCBI, ExPASy, EBI, Israel, Japan]
Duluc I., Boukamel R., Mantei N., Semenza G., Raul F., Freund J.-N.;
"Sequence of the precursor of intestinal lactase-phlorizin hydrolase from fetal rat.";
Gene 103:275-276(1991).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192.
STRAIN=Sprague-Dawley;
PubMed=1339333 [NCBI, ExPASy, EBI, Israel, Japan]
Boukamel R., Freund J.-N.;
"The rat LPH gene 5' region: comparative structure with the human gene.";
DNA Seq. 3:119-121(1992).
[3]
 NUCLEOTIDE SEQUENCE OF 1288-1370.
PubMed=1691182 [NCBI, ExPASy, EBI, Israel, Japan]
Bueller H.A., Kothe M.J., Goldman D.A., Grubman S.A., Sasak W.V., Matsudaira P.T., Montgomery R.K., Grand R.J.;
"Coordinate expression of lactase-phlorizin hydrolase mRNA and enzyme levels in rat intestine during development.";
J. Biol. Chem. 265:6978-6983(1990).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X56748; CAA40070.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X56747; CAA40069.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L04635; AAA41539.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JS0610; JS0610.
UniGene Rn.92376
3D structure databases
HSSP Q08638; 1OD0. [HSSP ENTRY / PDB]
ModBase Q02401.
Organism-specific databases
RGD 620823; Lct.
Gene expression databases
ArrayExpress Q02401; -.
GermOnline ENSRNOG00000003681; Rattus norvegicus.
Ontologies
GO
GO:0016324; Cellular component: apical plasma membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0043169; Molecular function: cation binding (inferred from electronic annotation from InterPro).
GO:0017042; Molecular function: glycosylceramidase activity (inferred from electronic annotation from EC).
GO:0000016; Molecular function: lactase activity (inferred from electronic annotation from EC).
GO:0005975; Biological process: carbohydrate metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001360; Glyco_hydro_1.
IPR013781; Glyco_hydro_sub_cat.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.80; Glyco_hydro_cat; 4.
PANTHER PTHR10353; Glyco_hydro_1; 1.
Pfam PF00232; Glyco_hydro_1; 3.
Pfam graphical view of domain structure.
PRINTS PR00131; GLHYDRLASE1.
ProDom PD000650; Glyco_hydro_euk; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00572; GLYCOSYL_HYDROL_F1_1; 1.
PS00653; GLYCOSYL_HYDROL_F1_2; 2.
ProtoNet Q02401.
Genome annotation databases
Ensembl ENSRNOG00000003681; Rattus norvegicus. [Contig view]
Phylogenomic databases
HOVERGEN Q02401; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Glycosidase; Hydrolase; Membrane; Multifunctional enzyme; Phosphoprotein; Repeat; Signal; Transmembrane; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
SIGNAL   1     19  19     By similarity. 
PROPEP   20    867  848     Beta-glucosidase. PRO_0000011771
CHAIN   868   1928  1061     Lactase-phlorizin hydrolase. PRO_0000011772
TOPO_DOM   22   1883  1862     Extracellular (Potential). 
TRANSMEM   1884   1902  19     Potential. 
TOPO_DOM   1903   1928  26     Cytoplasmic (Potential). 
REPEAT   89    175  87     1. 
REPEAT   365    849  485     2. 
REPEAT   886   1366  481     3. 
REPEAT   1371   1842  472     4. 
REGION   89   1842  1754     4 X approximate repeats. 
ACT_SITE   1067   1067        Proton donor (Potential). 
ACT_SITE   1274   1274        Nucleophile (By similarity). 
ACT_SITE   1539   1539        Proton donor (Potential). 
ACT_SITE   1750   1750        Nucleophile (By similarity). 
MOD_RES   1304   1304        Phosphotyrosine (By similarity). 
MOD_RES   1314   1314        Phosphotyrosine (By similarity). 
CONFLICT   7      7        A -> R (in Ref. 1; AAA41539). 
CONFLICT   113    113        Q -> E (in Ref. 1; AAA41539). 
CONFLICT   207    207        E -> D (in Ref. 1; AAA41539). 
CONFLICT   1337   1337        Y -> H (in Ref. 3). 
CONFLICT   1357   1359        PDL -> AEV (in Ref. 3). 
CONFLICT   1369   1370        RE -> GK (in Ref. 3). 
Sequence information
Length: 1928 AA [This is the length of the unprocessed precursor] Molecular weight: 217268 Da [This is the MW of the unprocessed precursor] CRC64: 56DDCAAC4ACAEA85 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MELPWTALFL STVLLGLSCQ GSDWESDRNF ISAAGPLTND LVLNLNYPPG KQGSDVVSGN 

        70         80         90        100        110        120 
TDHLLCQQPL PSFLSQYFSS LRASQVTHYK VLLSWAQLLP TGSSKNPDQE AVQCYRQLLQ 

       130        140        150        160        170        180 
SLKDAQLEPM VVLCHQTPPT SSAIQREGAF ADLFADYATL AFQSFGDLVE IWFTFSDLEK 

       190        200        210        220        230        240 
VIMDLPHKDL KASALQTLSN AHRRAFEIYH RKFSSQGGKL SVVLKAEDIP ELLPDPALAA 

       250        260        270        280        290        300 
LVQGSVDFLS LDLSYECQSV ATLPQKLSEL QNLEPKVKVF IYTLKLEDCP ATGTSPSSLL 

       310        320        330        340        350        360 
ISLLEAINKD QIQTVGFDVN AFLSCTSNSE ESPSCSLTDS LALQTEQQQE TAVPSSPGSA 

       370        380        390        400        410        420 
YQRVWAAFAN QSREERDAFL QDVFPEGFLW GISTGAFNVE GGWAEGGRGP SIWDHYGNLN 

       430        440        450        460        470        480 
AAEGQATAKV ASDSYHKPAS DVALLRGIRA QVYKFSISWS GLFPLGQKST PNRQGVAYYN 

       490        500        510        520        530        540 
KLIDRLLDSH IEPMATLFHW DLPQALQEQG GWQNESVVEA FLDYAAFCFS TFGDRVKLWV 

       550        560        570        580        590        600 
TFHEPWVMSY AGYGTGQHAP AISDPGMASF KVAHLILKAH ARTWHLYDLH HRLQQQGRVG 

       610        620        630        640        650        660 
IVLNSDLAEP LDRKSPQDLA AAERFLHFML GWFAHPIFVD GDYPTTSAQI QHINQQCGHP 

       670        680        690        700        710        720 
LAQLPEFTEA EKRLLKGSAD FLGLSHYTSR LISKAGRQTC TSSYDNIGGF SQHVDPEWPQ 

       730        740        750        760        770        780 
TASPWIRVVP WGIRRLLRFA SMEYTKGKLP IFLAGNGMPV GEEADLFDDS VRVNYFNWYI 

       790        800        810        820        830        840 
NEVLKAVKED LVDVRSYIVR SLIDGYEGPL GFSQRFGLYH VNFNDSSRPR TPRKSAYLFT 

       850        860        870        880        890        900 
SIIEKNGFSA KKVKRNPLPV RADFTSRARV TDSLPSEVPS KAKISVEKFS KQPRFERDLF 

       910        920        930        940        950        960 
YDGRFRDDFL WGVSSSPYQI EGGWNADGKG PSIWDNFTHT PGNGVKDNAT GDVACDSYHQ 

       970        980        990       1000       1010       1020 
LDADLNILRT LKVKSYRFSI SWSRIFPTGR NSTINKQGVD YYNRLIDSLV DNNIFPMVTL 

      1030       1040       1050       1060       1070       1080 
FHWDLPQALQ DIGGWENPSL IELFDSYADY CFKTFGDRVK FWMTFNEPWC HVVLGYSSGI 

      1090       1100       1110       1120       1130       1140 
FPPSVQEPGW LPYKVSHIVI KAHARVYHTY DEKYRSEQKG VISLSLNTHW AEPKDPGLQR 

      1150       1160       1170       1180       1190       1200 
DVEAADRMLQ FTMGWFAHPI FKNGDYPDVM KWTVGNRSEL QHLASSRLPT FTEEEKNYVR 

      1210       1220       1230       1240       1250       1260 
GTADVFCHNT YTSVFVQHST PRLNPPSYDD DMELKLIEMN SSTGVMHQDV PWGTRRLLNW 

      1270       1280       1290       1300       1310       1320 
IKEEYGNIPI YITENGQGLE NPTLDDTERI FYHKTYINEA LKAYKLDGVD LRGYSAWTLM 

      1330       1340       1350       1360       1370       1380 
DDFEWLLGYT MRFGLYYVDF NHVSRPRTAR ASARYYPDLI ANNGMPLARE DEFLYGEFPK 

      1390       1400       1410       1420       1430       1440 
GFIWSAASAS YQVEGAWRAD GKGLSIWDTF SHTPLRIGND DNGDVACDSY HKIAEDVVAL 

      1450       1460       1470       1480       1490       1500 
QNLGVSHYRF SIAWSRILPD GTTKFINEAG LSYYVRFIDA LLAAGITPQV TIYHWDLPQA 

      1510       1520       1530       1540       1550       1560 
LQDVGGWENE TIVQRFKEYA DVLFQRLGDR VKFWITLNEP FVIAAQGYGT GVSAPGISFR 

      1570       1580       1590       1600       1610       1620 
PGTAPYIAGH NLIKAHAEAW HLYNDVYRAR QGGTISITIS SDWGEPRDPT NREHVEAARS 

      1630       1640       1650       1660       1670       1680 
YVQFMGGWFA HPIFKNGDYP EVMKTRIRDR SLGAGLNKSR LPEFTESEKS RIKGTFDFFG 

      1690       1700       1710       1720       1730       1740 
FNHNTTVLAY NLDYPAAFSS FDADRGVASI ADSSWPVSGS FWLKVTPFGF RRILNWLKEE 

      1750       1760       1770       1780       1790       1800 
YNNPPIYVTE NGVSRRGEPE LNDTDRIYYL RSYINEALKA VHDKVDLRGY TVWSIMDNFE 

      1810       1820       1830       1840       1850       1860 
WATGFAERFG VHFVNRSDPS LPRIPRASAK FYATIVRCNG FPDPAQGPHP CLQQPEDAAP 

      1870       1880       1890       1900       1910       1920 
TASPVQSEVP FLGLMLGIAE AQTALYVLFA LLLLGACSLA FLTYNTGRRS KQGNAQPSQH 


QLSPISSF
Q02401 in FASTA format

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