NUCLEOTIDE SEQUENCE [MRNA], ACETYLATION AT THR-2, AND PARTIAL PROTEIN SEQUENCE.
TISSUE=Liver;
PubMed=1339442 [NCBI, ExPASy, EBI, Israel, Japan]
Endo F., Awata H., Tanoue A., Ishiguro M., Eda Y., Titani K., Matsuda I.;
"Primary structure deduced from complementary DNA sequence and expression in cultured cells of mammalian 4-hydroxyphenylpyruvic acid dioxygenase. Evidence that the enzyme is a homodimer of identical subunits homologous to rat liver-specific alloantigen F.";
J. Biol. Chem. 267:24235-24240(1992).

Comments

CATALYTIC ACTIVITY: 4-hydroxyphenylpyruvate + O₂ = homogentisate + CO₂.
COFACTOR: Binds 1 iron ion per subunit (By similarity).
PATHWAY: Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 3/6 .
SUBUNIT: Homodimer.
TISSUE SPECIFICITY: Liver and kidney.
SIMILARITY: Belongs to the 4HPPD family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D13390; BAA02660.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

S32821; S32821.

NP_999389.1; -.

3D structure databases

HSSP

Q8VQN0; 1JC4. [HSSP ENTRY / PDB]

SMR

Q02110; 8-366.

ModBase

Q02110.

Family and domain databases

InterPro

IPR005956; 4OHPhenylPyrv_dOase.
IPR004360; Glyas_bleo-R_dOase.
Graphical view of domain structure.

PANTHER

PTHR11959; HPP_dOase; 1.

Pfam

PF00903; Glyoxalase; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF009283; HPP_dOase; 1.

TIGRFAMs

TIGR01263; 4HPPD; 1.

BLOCKS

Q02110.

Genome annotation databases

GeneID

397443; -.

KEGG

ssc:397443; -.

Phylogenomic databases

HOVERGEN

Q02110; -.

Other

ProtoNet

Q02110.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Acetylation; Dioxygenase; Direct protein sequencing; Iron; Metal-binding; Oxidoreductase; Phenylalanine catabolism; Phosphoprotein; Tyrosine catabolism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 393`	`392`	`4-hydroxyphenylpyruvate dioxygenase.`	`PRO_0000088390`
`METAL`	`183 183`		`Iron (By similarity).`
`METAL`	`266 266`		`Iron (By similarity).`
`METAL`	`349 349`		`Iron (By similarity).`
`MOD_RES`	`2 2`		`N-acetylthreonine.`
`MOD_RES`	`250 250`		`Phosphoserine (By similarity).`

Sequence information

Length: 393 AA [This is the length of the unprocessed precursor]

Molecular weight: 45064 Da [This is the MW of the unprocessed precursor]

CRC64: C1A1D42EB2600182 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTSYSDKGEK PERGRFLHFH SVTFWVGNAK QAASYYCSKI GFEPLAYKGL ETGSREVVSH 

        70         80         90        100        110        120 
VVKQDKIVFV FSSALNPWNK EMGDHLVKHG DGVKDIAFEV EDCDYIVQKA RERGAIIVRE 

       130        140        150        160        170        180 
EVCCAADVRG HHTPLDRARQ VWEGTLVEKM TFCLDSRPQP SQTLLHRLLL SKLPKCGLEI 

       190        200        210        220        230        240 
IDHIVGNQPD QEMESASQWY MRNLQFHRFW SVDDTQIHTE YSALRSVVMA NYEESIKMPI 

       250        260        270        280        290        300 
NEPAPGKKKS QIQEYVDYNG GAGVQHIALK TEDIITAIRS LRERGVEFLA VPFTYYKQLQ 

       310        320        330        340        350        360 
EKLKSAKIRV KESIDVLEEL KILVDYDEKG YLLQIFTKPM QDRPTVFLEV IQRNNHQGFG 

       370        380        390 
AGNFNSLFKA FEEEQELRGN LTDTDPNGVP FRL

Q02110 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools