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[1]
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NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Wistar;
TISSUE=Ovary;
MacDuff P.E.,
Kerr L.E.,
Aitken R.J.;
"Molecular characterisation of zona pellucida protein 3 (ZP3) in the rat.";
J. Reprod. Fertil. Abstr. Ser. 18:86-86(1996).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 57-63, AND GLYCOSYLATION.
STRAIN=Sprague-Dawley;
TISSUE=Ovary;
DOI=10.1002/(SICI)1098-2795(199812)51:4<454::AID-MRD13>3.0.CO;2-G; PubMed=9820205 [NCBI, ExPASy, EBI, Israel, Japan]
Akatsuka K.,
Yoshida-Komiya H.,
Tulsiani D.R.P.,
Orgebin-Crist M.-C.,
Hiroi M.,
Araki Y.;
"Rat zona pellucida glycoproteins: molecular cloning and characterization of the three major components.";
Mol. Reprod. Dev. 51:454-467(1998).
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[3]
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PROTEIN SEQUENCE OF C-TERMINUS, PYROGLUTAMATE FORMATION AT GLN-23, DISULFIDE BOND FORMATION AT 78-CYS--CYS-98; 216-CYS--CYS-283 AND 240-CYS--CYS-301, GLYCOSYLATION AT ASN-146; ASN-273; ASN-304 AND ASN-330, AND MASS SPECTROMETRY.
DOI=10.1021/bi051883f; PubMed=16342937 [NCBI, ExPASy, EBI, Israel, Japan]
Boja E.S.,
Hoodbhoy T.,
Garfield M.,
Fales H.M.;
"Structural conservation of mouse and rat zona pellucida glycoproteins. Probing the native rat zona pellucida proteome by mass spectrometry.";
Biochemistry 44:16445-16460(2005).
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- FUNCTION: The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
- SUBUNIT: Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers (By similarity).
- SUBCELLULAR LOCATION: Processed zona pellucida sperm-binding protein 3: Secreted, extracellular space, extracellular matrix.
- SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
- TISSUE SPECIFICITY: Oocytes.
- DOMAIN: The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
- PTM: Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
- PTM: N-glycosylated; N-linked glycans are of high mannose/hybrid type, as well as bi-, tri- and tetra-antennary complex types.
- PTM: O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.
- SIMILARITY: Belongs to the ZP domain family. ZPC subfamily.
- SIMILARITY: Contains 1 ZP domain.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 424 AA [This is the length of the unprocessed precursor] |
Molecular weight: 45901 Da [This is the MW of the unprocessed precursor] |
CRC64: 2AB42CBB14DE8701 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MGPSCLLFLC LLLCGGPELC YPQTQWLLPG GTPTPAGSSS PVEVECKEAE LVVTVRRDLF
70 80 90 100 110 120
GTGKLVQPGD LTLGSEGCQP LVAVDTDVVR LNAQLHECSS GVQVTEDALV YNTFLLHDPR
130 140 150 160 170 180
PVNGLSILRT NRVEVPIECR YPRQGNVSSH PIQPTWVPFS ATVSSEEKLA FSLRLMEEDW
190 200 210 220 230 240
NTEKSSPTFH LGEVAHLQAE VQTGSHLPLQ LFVDHCVATP SPLPGQNSSP HHFIVDSHGC
250 260 270 280 290 300
LVDGLSESFS AFQVPRPRPE TLQFTVDVFH FANSSRNTVY ITCHLKVAPA NQIPDKLNKA
310 320 330 340 350 360
CSFNKTSQSW LPVEGDADIC DCCSNGNCSN SSSSEFETHE PAQWSTLVSR NRRHVTDEAD
370 380 390 400 410 420
VTVGPLIFLG KANDQAVEGW TSSAQTSVAL GLGLATVAFL TLAAIVLGVT RKCHTSSYLV
SLPQ
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P97708 in FASTA format |
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