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UniProtKB/Swiss-Prot entry P97287


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MCL1_MOUSE
Primary accession number P97287
Secondary accession numbers Q3TUS0 Q792P0 Q9CRI4
Integrated into Swiss-Prot on December 6, 2005
Sequence was last modified on November 1, 1998 (Sequence version 3)
Annotations were last modified on    September 23, 2008 (Entry version 71)
Name and origin of the protein
Protein name Induced myeloid leukemia cell differentiation protein Mcl-1 homolog
Synonym Bcl-2-related protein EAT/mcl1
Gene name
Name: Mcl1
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
TISSUE=Fetus;
DOI=10.1016/S0167-4781(98)00059-1; PubMed=9655929 [NCBI, ExPASy, EBI, Israel, Japan]
Okita H., Umezawa A., Suzuki A., Hata J.;
"Up-regulated expression of murine Mcl1/EAT, a bcl-2 related gene, in the early stage of differentiation of murine embryonal carcinoma cells and embryonic stem cells.";
Biochim. Biophys. Acta 1398:335-341(1998).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J;
TISSUE=Embryo, and Embryonic stem cell;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Czech II, and FVB/N;
TISSUE=Mammary tumor, and Salivary gland;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-65, AND INDUCTION.
STRAIN=129/SvJ;
PubMed=9671497 [NCBI, ExPASy, EBI, Israel, Japan]
Chao J.-R., Wang J.-M., Lee S.-F., Peng H.-W., Lin Y.-H., Chou C.-H., Li J.-C., Huang H.-M., Chou C.-K., Kuo M.-L., Yen J.J.-Y., Yang-Yen H.-F.;
"Mcl-1 is an immediate-early gene activated by the granulocyte-macrophage colony-stimulating factor (GM-CSF) signaling pathway and is one component of the GM-CSF viability response.";
Mol. Cell. Biol. 18:4883-4898(1998).
[5]
STRUCTURE BY NMR OF 152-308, AND INTERACTION WITH BCL2L11; BMF AND PMAIP.
DOI=10.1074/jbc.M411434200; PubMed=15550399 [NCBI, ExPASy, EBI, Israel, Japan]
Day C.L., Chen L., Richardson S.J., Harrison P.J., Huang D.C.S., Hinds M.G.;
"Solution structure of prosurvival Mcl-1 and characterization of its binding by proapoptotic BH3-only ligands.";
J. Biol. Chem. 280:4738-4744(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U35623; AAC31790.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK010424; BAB26927.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK159504; BAE35137.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK160594; BAE35901.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC003839; AAH03839.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC005427; AAH05427.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC021638; AAH21638.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF063886; AAC27929.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_032588.1; -.
UniGene Mm.1639
3D structure databases
PDB
1WSX; NMR; -; A=152-308.[ExPASy / RCSB / EBI]
2JM6; NMR; -; B=148-308.[ExPASy / RCSB / EBI]
2ROC; NMR; -; A=148-308.[ExPASy / RCSB / EBI]
2ROD; NMR; -; A=148-308.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1WSX; -.
2JM6; -.
2ROC; -.
2ROD; -.
ModBase P97287.
Protein-protein interaction databases
IntAct P97287; -.
PTM databases
PhosphoSite P97287; -.
Organism-specific databases
MGI MGI:101769; Mcl1.
Gene expression databases
ArrayExpress P97287; -.
CleanEx MM_MCL1; -.
GermOnline ENSMUSG00000038612; Mus musculus.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR013281; Apop_reg_Mc1.
IPR002475; BCL2_apoptsis.
IPR000712; Bcl2_BH.
Graphical view of domain structure.
Pfam PF00452; Bcl-2; 1.
Pfam graphical view of domain structure.
PRINTS PR01866; APOPREGMCL1.
PR01862; BCL2FAMILY.
SMART SM00337; BCL; 1.
SMART graphical view of domain structure.
PROSITE PS50062; BCL2_FAMILY; 1.
PS01080; BH1; 1.
PS01258; BH2; 1.
PS01259; BH3; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P97287.
Genome annotation databases
Ensembl ENSMUSG00000038612; Mus musculus. [Contig view]
GeneID 17210; -.
KEGG mmu:17210; -.
Phylogenomic databases
HOGENOM P97287; -.
HOVERGEN P97287; -.
Other
SOURCE Mcl1; Mus musculus.
ProtoNet P97287.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Apoptosis; Cytoplasm; Developmental protein; Differentiation; Membrane; Mitochondrion; Nucleus; Phosphoprotein; Transmembrane; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   331  331     Induced myeloid leukemia cell differentiation protein Mcl-1 homolog. PRO_0000143081
TRANSMEM   308   330  23     Potential. 
REGION   85   156  72     PEST-like (By similarity). 
MOTIF   190   204  15     BH3. 
MOTIF   233   253  21     BH1. 
MOTIF   285   300  16     BH2. 
COMPBIAS   149   152  4     Poly-Glu. 
SITE   108   109  2     Cleavage; by caspase-3 (By similarity). 
SITE   138   139  2     Cleavage; by caspase-3 (By similarity). 
MOD_RES   102   102        Phosphoserine (By similarity). 
MOD_RES   144   144        Phosphothreonine; by MAPK (By similarity). 
CROSSLNK   117   117        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) (By similarity). 
CROSSLNK   175   175        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) (By similarity). 
CROSSLNK   178   178        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) (By similarity). 
HELIX   154   172  19      
STRAND   181   183  3      
HELIX   185   215  31      
TURN   221   223  3      
HELIX   224   234  11      
STRAND   237   239  3      
HELIX   242   261  20      
HELIX   265   282  18      
HELIX   284   289  6      
TURN   290   292  3      
HELIX   293   299  7      
Sequence information
Length: 331 AA [This is the length of the unprocessed precursor] Molecular weight: 35217 Da [This is the MW of the unprocessed precursor] CRC64: E4FD369DB0EC6723 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MFGLRRNAVI GLNLYCGGAS LGAGGGSPAG ARLVAEEAKA RREGGGEAAL LPGARVVARP 

        70         80         90        100        110        120 
PPVGAEDPDV TASAERRLHK SPGLLAVPPE EMAASAAAAI VSPEEELDGC EPEAIGKRPA 

       130        140        150        160        170        180 
VLPLLERVSE AAKSSGADGS LPSTPPPPEE EEDDLYRQSL EIISRYLREQ ATGSKDSKPL 

       190        200        210        220        230        240 
GEAGAAGRRA LETLRRVGDG VQRNHETAFQ GMLRKLDIKN EGDVKSFSRV MVHVFKDGVT 

       250        260        270        280        290        300 
NWGRIVTLIS FGAFVAKHLK SVNQESFIEP LAETITDVLV RTKRDWLVKQ RGWDGFVEFF 

       310        320        330 
HVQDLEGGIR NVLLAFAGVA GVGAGLAYLI R 

P97287 in FASTA format

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