NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Bristol N2;
DOI=10.1126/science.282.5396.2012; PubMed=9851916 [NCBI, ExPASy, EBI, Israel, Japan]
The C. elegans sequencing consortium;
"Genome sequence of the nematode C. elegans: a platform for investigating biology.";
Science 282:2012-2018(1998).

[2]

GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-103, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M600392-MCP200; PubMed=17761667 [NCBI, ExPASy, EBI, Israel, Japan]
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.;
"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins.";
Mol. Cell. Proteomics 6:2100-2109(2007).

Comments

FUNCTION: Probable lyase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Mediates the dismutation of the unstable peptidyl(2-hydroxyglycine) intermediate to glyoxylate and the corresponding desglycine peptide amide. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity (By similarity).
CATALYTIC ACTIVITY: Peptidylamidoglycolate = peptidyl amide + glyoxylate.
COFACTOR: Zinc (By similarity).
SUBCELLULAR LOCATION: Secreted (Potential).
SIMILARITY: Belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
SIMILARITY: Contains 4 NHL repeats.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U88175; AAB42278.2; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

T25723; T25723.

NP_491475.2; -.

3D structure databases

ModBase

P91268.

Organism-specific databases

WormBase

WBGene00017671; F21F3.1.

WormPep

F21F3.1; CE32870. [WormPep / WorfDB]

Gene expression databases

ArrayExpress

P91268; -.

Ontologies

GO:0004598;	Molecular function: peptidylamidoglycolate lyase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR011042; 6-blade_b-propeller_TolB-like.
IPR001258; NHL_repeat.
IPR013017; NHL_repeat_subgr.
IPR000720; Pep_amidat_mOase.
Graphical view of domain structure.

Gene3D

G3DSA:2.120.10.30; 6-blade_b-propeller_TolB-like; 1.

Pfam

PF01436; NHL; 3.
Pfam graphical view of domain structure.

PRINTS

PR00790; PAMONOXGNASE.

PROSITE

PS51125; NHL; 4.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P91268.

Genome annotation databases

Ensembl

F21F3.1; Caenorhabditis elegans. [Contig view]

GeneID

172108; -.

KEGG

cel:F21F3.1; -.

NMPDR

fig|6239.3.peg.1034; -.

Other

ProtoNet

P91268.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Glycoprotein; Lyase; Metal-binding; Repeat; Secreted; Signal; Zinc.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 19`	`19`	`Potential.`
`CHAIN`	`20 350`	`331`	`Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase F21F3.1.`	`PRO_0000248572`
`REPEAT`	`46 90`	`45`	`NHL 1.`
`REPEAT`	`113 154`	`42`	`NHL 2.`
`REPEAT`	`162 206`	`45`	`NHL 3.`
`REPEAT`	`212 256`	`45`	`NHL 4.`
`CARBOHYD`	`103 103`		`N-linked (GlcNAc...).`
`DISULFID`	`176 196`		`By similarity.`
`DISULFID`	`241 252`		`By similarity.`

Sequence information

Length: 350 AA [This is the length of the unprocessed precursor]

Molecular weight: 38593 Da [This is the MW of the unprocessed precursor]

CRC64: A1892DDAAF0756DB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRASTACLVA LLAPFYISAL PVEYFYGDEQ QPIEEGAENS AVFEQDRELI GLFNPSKEIG 

        70         80         90        100        110        120 
QVSGLAVNKN GHIVAFHRSG RVWDEKSFND HETFNKDLGV INNKTIAIIS REKKVIDEFG 

       130        140        150        160        170        180 
AGLFYMPHGL TIDNNGDYWV TDVGSHQVHK IDAKTQKIVM SLGEKMVPGE DQAHFCKPTD 

       190        200        210        220        230        240 
VAVAKNGHIF VADGYCNSRI LKFDAKGNLM AQINAATEEN QPSEFVVPHS LSLIEDMNIV 

       250        260        270        280        290        300 
CVADRENQRV QCFSAGLSEG DRTLPTGIPI TSATDIGRVF AIREREHYLI GVTGNSEDVE 

       310        320        330        340        350 
AQMFSIDMQT GKTETFAKGV RNTHALAIAA DGVMFVSQLE PSRILEIRLL

P91268 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools