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UniProtKB/Swiss-Prot entry P84076

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name HPCA_RAT
Primary accession number P84076
Secondary accession numbers P32076 P41211 P70510
Integrated into Swiss-Prot on August 16, 2004
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 41)
Name and origin of the protein
Protein name Neuron-specific calcium-binding protein hippocalcin
Synonym P23K
Gene name
Name: Hpca
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
STRAIN=Wistar;
TISSUE=Brain;
DOI=10.1016/0006-291X(92)91587-G; PubMed=1280427 [NCBI, ExPASy, EBI, Israel, Japan]
Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T.;
"Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus.";
Biochem. Biophys. Res. Commun. 189:511-517(1992).
[2]
 SEQUENCE REVISION.
DOI=10.1006/bbrc.1993.2351; PubMed=8240319 [NCBI, ExPASy, EBI, Israel, Japan]
Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T.;
"Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus.";
Biochem. Biophys. Res. Commun. 196:1017-1017(1993).
[3]
 NUCLEOTIDE SEQUENCE.
STRAIN=Sprague-Dawley;
TISSUE=Olfactory epithelium;
DOI=10.1016/j.bbrc.2004.07.123; PubMed=15336960 [NCBI, ExPASy, EBI, Israel, Japan]
Mammen A., Simpson P.J., Nighorn A., Imanishi Y., Palczewski K., Ronnett G.V., Moon C.;
"Hippocalcin in the olfactory epithelium: a mediator of second messenger signaling.";
Biochem. Biophys. Res. Commun. 322:1131-1139(2004).
[4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Brain;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
 PRELIMINARY PROTEIN SEQUENCE OF 51-63; 101-116 AND 138-145, AND CHARACTERIZATION.
TISSUE=Brain;
DOI=10.1016/0006-291X(92)91635-4; PubMed=1543495 [NCBI, ExPASy, EBI, Israel, Japan]
Takamatsu K., Kitamura K., Noguchi T.;
"Isolation and characterization of recoverin-like Ca(2+)-binding protein from rat brain.";
Biochem. Biophys. Res. Commun. 183:245-251(1992).
[6]
 NUCLEOTIDE SEQUENCE [MRNA] OF 1-37.
STRAIN=Sprague-Dawley;
TISSUE=Testis;
DOI=10.1016/0306-4522(96)00344-2; PubMed=8938744 [NCBI, ExPASy, EBI, Israel, Japan]
Grant A.L., Jones A., Thomas K.L., Wisden W.;
"Characterization of the rat hippocalcin gene: the 5' flanking region directs expression to the hippocampus.";
Neuroscience 75:1099-1115(1996).
[7]
 PROTEIN SEQUENCE OF 51-94; 138-148; 164-171 AND 175-181, AND MASS SPECTROMETRY.
STRAIN=Sprague-Dawley;
TISSUE=Brain;
Lubec G., Kang S.U.;
Submitted (JUL-2007) to UniProtKB.
[8]
 MYRISTOYLATION AT GLY-2.
PubMed=8360179 [NCBI, ExPASy, EBI, Israel, Japan]
Kobayashi M., Takamatsu K., Saitoh S., Noguchi T.;
"Myristoylation of hippocalcin is linked to its calcium-dependent membrane association properties.";
J. Biol. Chem. 268:18898-18904(1993).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D12573; BAA02122.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY442172; AAR14053.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC087632; AAH87632.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X96993; CAA65718.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JC1347; JC1347.
PS0344; PS0344.
RefSeq NP_058818.1; -.
UniGene Rn.11019
3D structure databases
HSSP P29554; 1BJF. [HSSP ENTRY / PDB]
SMR P84076; 5-185.
ModBase P84076.
Protein-protein interaction databases
IntAct P84076; -.
PTM databases
PhosphoSite P84076; -.
Organism-specific databases
RGD 620060; Hpca.
Gene expression databases
ArrayExpress P84076; -.
GermOnline ENSRNOG00000006979; Rattus norvegicus.
Ontologies
GO
GO:0003779; Molecular function: actin binding (inferred from sequence or structural similarity from UniProtKB).
GO:0005509; Molecular function: calcium ion binding (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR011992; EF-Hand_type.
IPR002048; EF_hand_Ca_bd.
IPR001125; Recoverin.
Graphical view of domain structure.
Gene3D G3DSA:1.10.238.10; EF-Hand_type; 1.
Pfam PF00036; efhand; 3.
Pfam graphical view of domain structure.
PRINTS PR00450; RECOVERIN.
ProDom PD000012; EF-hand; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00054; EFh; 3.
SMART graphical view of domain structure.
PROSITE PS00018; EF_HAND_1; 3.
PS50222; EF_HAND_2; 3.
PROSITE graphical view of domain structure (profiles).
BLOCKS P84076.
Genome annotation databases
Ensembl ENSRNOG00000006979; Rattus norvegicus. [Contig view]
GeneID 29177; -.
KEGG rno:29177; -.
Phylogenomic databases
HOVERGEN P84076; -.
Other
ProtoNet P84076.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Direct protein sequencing; Lipoprotein; Myristate; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   193  192     Neuron-specific calcium-binding protein hippocalcin. PRO_0000073770
DOMAIN   24    59  36     EF-hand 1. 
DOMAIN   60    95  36     EF-hand 2. 
DOMAIN   96   131  36     EF-hand 3. 
DOMAIN   144   179  36     EF-hand 4. 
CA_BIND   73    84  12     1 (Potential). 
CA_BIND   109   120  12     2 (Potential). 
CA_BIND   157   168  12     3 (Potential). 
LIPID   2     2        N-myristoyl glycine. 
CONFLICT   60    60        D -> G (in Ref. 5; AA sequence). 
CONFLICT   102   102        M -> N (in Ref. 5; AA sequence). 
CONFLICT   113   113        N -> D (in Ref. 5; AA sequence). 
CONFLICT   142   143        ES -> TF (in Ref. 5; AA sequence). 
Sequence information
Length: 193 AA [This is the length of the unprocessed precursor] Molecular weight: 22427 Da [This is the MW of the unprocessed precursor] CRC64: 3DBEA176AC945DB1 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MGKQNSKLRP EMLQDLRENT EFSELELQEW YKGFLKDCPT GILNVDEFKK IYANFFPYGD 

        70         80         90        100        110        120 
ASKFAEHVFR TFDTNSDGTI DFREFIIALS VTSRGRLEQK LMWAFSMYDL DGNGYISREE 

       130        140        150        160        170        180 
MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV 

       190 
RLLQCDPSSA SQF
P84076 in FASTA format

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