[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15. STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1; PubMed=9348040 [NCBI, ExPASy, EBI, Israel, Japan] Uemori T., Sato Y., Kato I., Doi H., Ishino Y.; "A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization."; Genes Cells 2:499-512(1997).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1; Weiss R.B., Dunn D.M., Robb F.T., Brown J.R.; "The complete sequence of the Pyrococcus furiosus genome."; Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
[3]	CHARACTERIZATION. STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1; PubMed=8787781 [NCBI, ExPASy, EBI, Israel, Japan] Imamura M., Uemori T., Kato I., Doi H., Ishino Y.; "A non-alpha-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus."; Biol. Pharm. Bull. 18:1647-1652(1995).

Comments

FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
SUBUNIT: Heterodimer of a large subunit and a small subunit.
SIMILARITY: Belongs to the archaeal DNA polymerase II family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D84670; BAA25164.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AE010127; AAL80143.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T43934; T43934.

RefSeq

NP_577748.2; -.

3D structure databases

ModBase

P81409.

Ontologies

GO:0008408;	Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887;	Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738;	Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261;	Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00324; -; 1.
PBIL [Tree]

InterPro

IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
IPR013083; Znf_RING/FYVE/PHD.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.40.10; Znf_RING/FYVE/PHD; 1.

Pfam

PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF016275; PolC_DP2; 1.

TIGRFAMs

TIGR00354; polC; 1.

BLOCKS

P81409.

Genome annotation databases

1467847; -.

AE009950_GR; PF0019.

pfu:PF0019; -.

fig|186497.1.peg.19; -.

Phylogenomic databases

HOGENOM

P81409; -.

Genome annotation databases

CMR

P81409; PF0019.

Other

ProtoNet

P81409.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Direct protein sequencing; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1263`	`1263`	`DNA polymerase II large subunit.`	`PRO_0000152579`
`COMPBIAS`	`1235 1252`	`18`	`Lys-rich.`
`CONFLICT`	`1030 1030`		`G -> R (in Ref. 1; BAA25164).`
`CONFLICT`	`1034 1034`		`R -> G (in Ref. 1; BAA25164).`

Sequence information

Length: 1263 AA [This is the length of the unprocessed precursor]

Molecular weight: 143169 Da [This is the MW of the unprocessed precursor]

CRC64: 3BFFB2CC8D054F4C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MELPKEIEEY FEMLQREIDK AYEIAKKARS QGKDPSTDVE IPQATDMAGR VESLVGPPGV 

        70         80         90        100        110        120 
AQRIRELLKE YDKEIVALKI VDEIIEGKFG DFGSKEKYAE QAVRTALAIL TEGIVSAPLE 

       130        140        150        160        170        180 
GIADVKIKRN TWADNSEYLA LYYAGPIRSS GGTAQALSVL VGDYVRRKLG LDRFKPSGKH 

       190        200        210        220        230        240 
IERMVEEVDL YHRAVSRLQY HPSPDEVRLA MRNIPIEITG EATDDVEVSH RDVEGVETNQ 

       250        260        270        280        290        300 
LRGGAILVLA EGVLQKAKKL VKYIDKMGID GWEWLKEFVE AKEKGEEIEE SESKAEESKV 

       310        320        330        340        350        360 
ETRVEVEKGF YYKLYEKFRA EIAPSEKYAK EIIGGRPLFA GPSENGGFRL RYGRSRVSGF 

       370        380        390        400        410        420 
ATWSINPATM VLVDEFLAIG TQMKTERPGK GAVVTPATTA EGPIVKLKDG SVVRVDDYNL 

       430        440        450        460        470        480 
ALKIRDEVEE ILYLGDAIIA FGDFVENNQT LLPANYVEEW WIQEFVKAVN EAYEVELRPF 

       490        500        510        520        530        540 
EENPRESVEE AAEYLEVDPE FLAKMLYDPL RVKPPVELAI HFSEILEIPL HPYYTLYWNT 

       550        560        570        580        590        600 
VNPKDVERLW GVLKDKATIE WGTFRGIKFA KKIEISLDDL GSLKRTLELL GLPHTVREGI 

       610        620        630        640        650        660 
VVVDYPWSAA LLTPLGNLEW EFKAKPFYTV IDIINENNQI KLRDRGISWI GARMGRPEKA 

       670        680        690        700        710        720 
KERKMKPPVQ VLFPIGLAGG SSRDIKKAAE EGKIAEVEIA FFKCPKCGHV GPETLCPECG 

       730        740        750        760        770        780 
IRKELIWTCP KCGAEYTNSQ AEGYSYSCPK CNVKLKPFTK RKIKPSELLN RAMENVKVYG 

       790        800        810        820        830        840 
VDKLKGVMGM TSGWKIAEPL EKGLLRAKNE VYVFKDGTIR FDATDAPITH FRPREIGVSV 

       850        860        870        880        890        900 
EKLRELGYTH DFEGKPLVSE DQIVELKPQD VILSKEAGKY LLRVARFVDD LLEKFYGLPR 

       910        920        930        940        950        960 
FYNAEKMEDL IGHLVIGLAP HTSAGIVGRI IGFVDALVGY AHPYFHAAKR RNCDGDEDSV 

       970        980        990       1000       1010       1020 
MLLLDALLNF SRYYLPEKRG GKMDAPLVIT TRLDPREVDS EVHNMDVVRY YPLEFYEATY 

      1030       1040       1050       1060       1070       1080 
ELKSPKELVG VIERVEDRLG KPEMYYGIKF THDTDDIALG PKMSLYKQLG DMEEKVKRQL 

      1090       1100       1110       1120       1130       1140 
TLAERIRAVD QHYVAETILN SHLIPDLRGN LRSFTRQEFR CVKCNTKYRR PPLDGKCPVC 

      1150       1160       1170       1180       1190       1200 
GGKIVLTVSK GAIEKYLGTA KMLVANYNVK PYTRQRICLT EKDIDSLFEY LFPEAQLTLI 

      1210       1220       1230       1240       1250       1260 
VDPNDICMKM IKERTGETVQ GGLLENFNSS GNNGKKIEKK EKKAKEKPKK KKVISLDDFF 


SKR

P81409 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools