NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Venom gland;
Wang Q.Y., Shu Y.Y.;
"Cloning and characterization of cDNAs encoding two acidic isoforms of phospholipase A2 in Guangxi King Cobra (Ophiophagus hannah).";
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.

[2]

PROTEIN SEQUENCE OF 28-151.
TISSUE=Venom;
DOI=10.1006/abbi.1996.9814; PubMed=9028866 [NCBI, ExPASy, EBI, Israel, Japan]
Huang M.Z., Gopalakrishnakone P., Chung M.C.M., Kini R.M.;
"Complete amino acid sequence of an acidic, cardiotoxic phospholipase A2 from the venom of Ophiophagus hannah (King Cobra): a novel cobra venom enzyme with 'pancreatic loop'.";
Arch. Biochem. Biophys. 338:150-156(1997).

Comments

FUNCTION: PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Exhibits cardiotoxicity, myotoxicity, antiplatelet activity, and edema-inducing activity.
CATALYTIC ACTIVITY: Phosphatidylcholine + H₂O = 1-acylglycerophosphocholine + a carboxylate.
COFACTOR: Binds 1 calcium ion per subunit (By similarity).
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed by the venom gland.
SIMILARITY: Belongs to the phospholipase A2 family. Group I subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF302908; AAG23964.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

PDB

1GP7; X-ray; 2.60 A; A/B/C=1-151.

[ExPASy / RCSB / EBI]

PDBsum

1GP7; -.

ModBase

P80966.

Family and domain databases

InterPro

IPR016090; Phospholipase_A2.
IPR013090; Phospholipase_A2_AS.
IPR001211; Phospholipase_A2_euk.
Graphical view of domain structure.

Gene3D

G3DSA:1.20.90.10; Phospholipase_A2; 1.

PANTHER

PTHR11716; Phospholipase_A2; 1.

Pfam

PF00068; Phospholip_A2_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00389; PHPHLIPASEA2.

ProDom

PD000303; PhospholipaseA2; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00085; PA2c; 1.
SMART graphical view of domain structure.

PROSITE

PS00119; PA2_ASP; 1.
PS00118; PA2_HIS; 1.

BLOCKS

P80966.

Phylogenomic databases

HOVERGEN

P80966; -.

Other

ProtoNet

P80966.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Calcium; Cardiotoxin; Direct protein sequencing; Hydrolase; Lipid degradation; Metal-binding; Secreted; Signal; Toxin.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 21`	`21`	`Potential.`
`PROPEP`	`22 27`	`6`		`PRO_0000022942`
`CHAIN`	`28 151`	`124`	`Phospholipase A2, acidic 1.`	`PRO_0000022943`
`ACT_SITE`	`75 75`		`By similarity.`
`ACT_SITE`	`126 126`		`By similarity.`
`METAL`	`55 55`		`Calcium; via carbonyl oxygen (By similarity).`
`METAL`	`57 57`		`Calcium; via carbonyl oxygen (By similarity).`
`METAL`	`59 59`		`Calcium; via carbonyl oxygen (By similarity).`
`METAL`	`76 76`		`Calcium (By similarity).`
`DISULFID`	`38 104`		`By similarity.`
`DISULFID`	`54 151`		`By similarity.`
`DISULFID`	`56 72`		`By similarity.`
`DISULFID`	`71 132`		`By similarity.`
`DISULFID`	`78 125`		`By similarity.`
`DISULFID`	`88 118`		`By similarity.`
`DISULFID`	`111 123`		`By similarity.`
`HELIX`	`29 39`	`11`
`HELIX`	`46 49`	`4`
`TURN`	`53 55`	`3`
`STRAND`	`56 59`	`4`
`HELIX`	`67 84`	`18`
`HELIX`	`86 88`	`3`
`HELIX`	`94 97`	`4`
`STRAND`	`102 105`	`4`
`STRAND`	`108 111`	`4`
`HELIX`	`117 134`	`18`
`HELIX`	`140 142`	`3`
`HELIX`	`147 150`	`4`

Sequence information

Length: 151 AA [This is the length of the unprocessed precursor]

Molecular weight: 16444 Da [This is the MW of the unprocessed precursor]

CRC64: F1E021B886BC4BEB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNPAHLLVLS AVCVSLLGAS SIPPQPLHLI QFGNMIQCTV PGFLSWIKYA DYGCYCGAGG 

        70         80         90        100        110        120 
SGTPVDKLDR CCQVHDNCYT QAQKLPACSS IMDSPYVKIY SYDCSERTVT CKADNDECAA 

       130        140        150 
FICNCDRVAA HCFAASPYNN NNYNIDTTTR C

P80966 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools