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UniProtKB/Swiss-Prot entry P80370

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DLK1_HUMAN
Primary accession number P80370
Secondary accession numbers P15803 Q96DW5
Integrated into Swiss-Prot on November 1, 1995
Sequence was last modified on February 1, 1996 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 99)
Name and origin of the protein
Protein name Protein delta homolog 1 [Precursor]
Synonyms DLK-1
pG2
Contains Fetal antigen 1
     (FA1)
Gene name
Name: DLK1
Synonyms: DLK
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Adrenal gland;
PubMed=8095043 [NCBI, ExPASy, EBI, Israel, Japan]
Laborda J., Sausville E.A., Hoffman T., Notario V.;
"dlk, a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line.";
J. Biol. Chem. 268:3817-3820(1993).
[2]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Adrenal gland, and Placenta;
PubMed=7711066 [NCBI, ExPASy, EBI, Israel, Japan]
Lee Y.L., Helman L., Hoffman T., Laborda J.;
"dlk, pG2 and Pref-1 mRNAs encode similar proteins belonging to the EGF-like superfamily. Identification of polymorphic variants of this RNA.";
Biochim. Biophys. Acta 1261:223-232(1995).
[3]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Adrenal gland;
DOI=10.1093/nar/18.3.685; PubMed=2308864 [NCBI, ExPASy, EBI, Israel, Japan]
Helman L.J., Sack N., Plon S., Israel M.A.;
"The sequence of an adrenal specific human cDNA, pG2.";
Nucleic Acids Res. 18:685-685(1990).
[4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Adrenal gland;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
 PROTEIN SEQUENCE OF 24-383, AND GLYCOSYLATION AT SER-94; ASN-100; THR-143; SER-163; ASN-165; ASN-172; SER-214; THR-222; SER-251 AND SER-260.
TISSUE=Amniotic fluid;
DOI=10.1111/j.1432-1033.1994.00083.x; PubMed=7925474 [NCBI, ExPASy, EBI, Israel, Japan]
Jensen C.H., Krogh T.N., Hoejrup P., Clausen P.P., Skjoedt K., Larsson L.-I., Enghild J.J., Teisner B.;
"Protein structure of fetal antigen 1 (FA1). A novel circulating human epidermal-growth-factor-like protein expressed in neuroendocrine tumors and its relation to the gene products of dlk and pG2.";
Eur. J. Biochem. 225:83-92(1994).
[6]
 PROTEIN SEQUENCE OF 24-60.
TISSUE=Amniotic fluid;
PubMed=8501199 [NCBI, ExPASy, EBI, Israel, Japan]
Jensen C.H., Teisner B., Hoejrup P., Rasmussen H.B., Madsen O.D., Nielsen B., Skjoedt K.;
"Studies on the isolation, structural analysis and tissue localization of fetal antigen 1 and its relation to a human adrenal-specific cDNA, pG2.";
Hum. Reprod. 8:635-641(1993).
[7]
 PROTEIN SEQUENCE OF 24-38.
DOI=10.1110/ps.04682504; PubMed=15340161 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang Z., Henzel W.J.;
"Signal peptide prediction based on analysis of experimentally verified cleavage sites.";
Protein Sci. 13:2819-2824(2004).
Comments
  • FUNCTION: May have a role in neuroendocrine differentiation.
  • SUBUNIT: Monomer.
  • SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
  • ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing. Additional isoforms seem to exist.
    NameLong
    Isoform IDP80370-1
    This is the isoform sequence displayed in this entry.
    NameShort
    Isoform IDP80370-2
    Features which should be applied to build the isoform sequence: VSP_001377.
  • TISSUE SPECIFICITY: Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
  • PTM: N- and O-glycosylated.
  • SIMILARITY: Contains 6 EGF-like domains.
  • SEQUENCE CAUTION:
    • Sequence=CAA35582.1; Type=Frameshift; Positions=23, 45, 108, 199;
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z12172; CAA78163.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U15979; AAA75364.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U15981; AAA75365.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X17544; CAA35582.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC013197; AAH13197.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00160384; -.
IPI00218210; -.
PIR S53716; S53716.
S71548; S71548.
UniGene Hs.533717
3D structure databases
HSSP P08709; 1BF9. [HSSP ENTRY / PDB]
ModBase P80370.
Organism-specific databases
GeneCards GC14P100263; -.
HGNC HGNC:2907; DLK1.
GenAtlas DLK1.
HPA CAB005872; -.
MIM 176290; gene. [NCBI / EBI]
Orphanet 96184; Uniparental disomy of maternal origin, chromosome 14.
96334; Uniparental disomy of paternal origin, chromosome 14.
PharmGKB PA27363; -.
Gene expression databases
ArrayExpress P80370; -.
Bgee P80370; -.
CleanEx HS_DLK1; -.
GermOnline ENSG00000185559; Homo sapiens.
Ontologies
GO
GO:0005615; Cellular component: extracellular space (traceable author statement from ProtInc).
GO:0016021; Cellular component: integral to membrane (traceable author statement from ProtInc).
GO:0005625; Cellular component: soluble fraction (traceable author statement from ProtInc).
GO:0007275; Biological process: multicellular organismal development (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR006209; EGF.
IPR006210; EGF-like.
IPR013032; EGF-like_reg_CS.
IPR000152; EGF-type_Asp/Asn_hydroxyl_CS.
IPR001438; EGF_2.
IPR000742; EGF_3.
IPR013111; EGF_extracell.
Graphical view of domain structure.
Pfam PF00008; EGF; 4.
PF07974; EGF_2; 1.
Pfam graphical view of domain structure.
PRINTS PR00010; EGFBLOOD.
SMART SM00181; EGF; 6.
SMART graphical view of domain structure.
PROSITE PS00010; ASX_HYDROXYL; 1.
PS00022; EGF_1; 5.
PS01186; EGF_2; 5.
PS50026; EGF_3; 5.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE P80370; -.
Genome annotation databases
Ensembl ENSG00000185559; Homo sapiens. [Contig view]
Phylogenomic databases
HOGENOM P80370; -.
HOVERGEN P80370; -.
Other
SOURCE DLK1; Homo sapiens.
ProtoNet P80370.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Direct protein sequencing; Disulfide bond; EGF-like domain; Glycoprotein; Membrane; Polymorphism; Repeat; Signal; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    23  23      
CHAIN   24   383  360     Protein delta homolog 1. PRO_0000007518
CHAIN   24   303  280     Fetal antigen 1. PRO_0000007519
TOPO_DOM   24   303  280     Extracellular (Potential). 
TRANSMEM   304   327  24     Potential. 
TOPO_DOM   328   383  56     Cytoplasmic (Potential). 
DOMAIN   24    55  32     EGF-like 1. 
DOMAIN   53    86  34     EGF-like 2. 
DOMAIN   88   125  38     EGF-like 3. 
DOMAIN   127   168  42     EGF-like 4. 
DOMAIN   170   206  37     EGF-like 5. 
DOMAIN   208   245  38     EGF-like 6. 
CARBOHYD   94    94        O-linked (GalNAc...). 
CARBOHYD   100   100        N-linked (GlcNAc...). 
CARBOHYD   143   143        O-linked (GalNAc...). 
CARBOHYD   163   163        O-linked (GalNAc...); partial. 
CARBOHYD   165   165        N-linked (GlcNAc...); partial; atypical. 
CARBOHYD   172   172        N-linked (GlcNAc...); partial; atypical. 
CARBOHYD   214   214        O-linked (GalNAc...). 
CARBOHYD   222   222        O-linked (GalNAc...); partial. 
CARBOHYD   251   251        O-linked (GalNAc...); partial. 
CARBOHYD   260   260        O-linked (GalNAc...); partial. 
DISULFID   26    37        By similarity. 
DISULFID   30    43        By similarity. 
DISULFID   45    54        By similarity. 
DISULFID   57    68        By similarity. 
DISULFID   63    74        By similarity. 
DISULFID   76    85        By similarity. 
DISULFID   92   103        By similarity. 
DISULFID   97   113        By similarity. 
DISULFID   115   124        By similarity. 
DISULFID   131   144        By similarity. 
DISULFID   138   156        By similarity. 
DISULFID   158   167        By similarity. 
DISULFID   174   185        By similarity. 
DISULFID   179   194        By similarity. 
DISULFID   196   205        By similarity. 
DISULFID   212   223        By similarity. 
DISULFID   217   233        By similarity. 
DISULFID   235   244        By similarity. 
VAR_SEQ   229   301        Missing (in isoform Short). VSP_001377
VARIANT   73    73  1     Q -> L (in dbSNP:rs34686110 [NCBI]). VAR_055715 
VARIANT   104   104  1     V -> M (in dbSNP:rs2273607 [NCBI]). VAR_055716 
VARIANT   260   260  1     S -> N (in dbSNP:rs1058009 [NCBI]). VAR_055717 
VARIANT   347   347  1     Missing (in clone HDLKAAG). VAR_002274
CONFLICT   45    47        Missing (in Ref. 3). 
CONFLICT   46    47        QP -> HV (in Ref. 1; CAA78163). 
CONFLICT   108   108        G -> D (in Ref. 4 and 5). 
Sequence information
Length: 383 AA [This is the length of the unprocessed precursor] Molecular weight: 41143 Da [This is the MW of the unprocessed precursor] CRC64: 2C55C6E355B4415B [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTATEALLRV LLLLLAFGHS TYGAECFPAC NPQNGFCEDD NVCRCQPGWQ GPLCDQCVTS 

        70         80         90        100        110        120 
PGCLHGLCGE PGQCICTDGW DGELCDRDVR ACSSAPCANN GTCVSLDGGL YECSCAPGYS 

       130        140        150        160        170        180 
GKDCQKKDGP CVINGSPCQH GGTCVDDEGR ASHASCLCPP GFSGNFCEIV ANSCTPNPCE 

       190        200        210        220        230        240 
NDGVCTDIGG DFRCRCPAGF IDKTCSRPVT NCASSPCQNG GTCLQHTQVS YECLCKPEFT 

       250        260        270        280        290        300 
GLTCVKKRAL SPQQVTRLPS GYGLAYRLTP GVHELPVQQP EHRILKVSMK ELNKKTPLLT 

       310        320        330        340        350        360 
EGQAICFTIL GVLTSLVVLG TVGIVFLNKC ETWVSNLRYN HMLRKKKNLL LQYNSGEDLA 

       370        380 
VNIIFPEKID MTTFSKEAGD EEI
P80370 in FASTA format

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