[1]	PROTEIN SEQUENCE, AND FUNCTION. TISSUE=Skin secretion; PubMed=8306981 [NCBI, ExPASy, EBI, Israel, Japan] Mor A., Nicolas P.; "Isolation and structure of novel defensive peptides from frog skin."; Eur. J. Biochem. 219:145-154(1994).
[2]	FUNCTION. DOI=10.1074/jbc.272.50.31609; PubMed=9395500 [NCBI, ExPASy, EBI, Israel, Japan] Ghosh J.K., Shaool D., Guillaud P., Ciceron L., Mazier D., Kustanovich I., Shai Y., Mor A.; "Selective cytotoxicity of dermaseptin S3 toward intraerythrocytic Plasmodium falciparum and the underlying molecular basis."; J. Biol. Chem. 272:31609-31616(1997).
[3]	POTENTIAL THERAPEUTIC USAGE. DOI=10.1016/S0196-9781(01)00504-6; PubMed=11587797 [NCBI, ExPASy, EBI, Israel, Japan] Feder R., Nehushtai R., Mor A.; "Affinity driven molecular transfer from erythrocyte membrane to target cells."; Peptides 22:1683-1690(2001).
[4]	POTENTIAL THERAPEUTIC USAGE IN TREATMENT OF BACTERIAL INFECTIONS. DOI=10.1128/AAC.46.3.689-694.2002; PubMed=11850249 [NCBI, ExPASy, EBI, Israel, Japan] Navon-Venezia S., Feder R., Gaidukov L., Carmeli Y., Mor A.; "Antibacterial properties of dermaseptin S4 derivatives with in vivo activity."; Antimicrob. Agents Chemother. 46:689-694(2002).
[5]	POTENTIAL THERAPEUTIC USAGE. DOI=10.1021/bi0201466; PubMed=12119035 [NCBI, ExPASy, EBI, Israel, Japan] Hariton-Gazal E., Feder R., Mor A., Graessmann A., Brack-Werner R., Jans D., Gilon C., Loyter A.; "Targeting of nonkaryophilic cell-permeable peptides into the nuclei of intact cells by covalently attached nuclear localization signals."; Biochemistry 41:9208-9214(2002).
[6]	POTENTIAL THERAPEUTIC USAGE IN TREATMENT OF MALARIA. DOI=10.1074/jbc.M202089200; PubMed=11937508 [NCBI, ExPASy, EBI, Israel, Japan] Efron L., Dagan A., Gaidukov L., Ginsburg H., Mor A.; "Direct interaction of dermaseptin S4 aminoheptanoyl derivative with intraerythrocytic malaria parasite leading to increased specific antiparasitic activity in culture."; J. Biol. Chem. 277:24067-24072(2002).
[7]	POTENTIAL THERAPEUTIC USAGE IN PREVENTION OF HERPES SIMPLEX VIRUS TYPE 1 INFECTION. DOI=10.1002/jmv.2134; PubMed=11782932 [NCBI, ExPASy, EBI, Israel, Japan] Belaid A., Aouni M., Khelifa R., Trabelsi A., Jemmali M., Hani K.; "In vitro antiviral activity of dermaseptins against herpes simplex virus type 1."; J. Med. Virol. 66:229-234(2002).
[8]	POTENTIAL THERAPEUTIC USAGE IN PREVENTION OF HIV-1 INFECTION. DOI=10.1016/j.virol.2005.02.002; PubMed=15780876 [NCBI, ExPASy, EBI, Israel, Japan] Lorin C., Saidi H., Belaid A., Zairi A., Baleux F., Hocini H., Belec L., Hani K., Tangy F.; "The antimicrobial peptide dermaseptin S4 inhibits HIV-1 infectivity in vitro."; Virology 334:264-275(2005).
[9]	POTENTIAL THERAPEUTIC USAGE IN TREATMENT OF BACTERIAL INFECTIONS. DOI=10.1128/AAC.00030-06; PubMed=16870756 [NCBI, ExPASy, EBI, Israel, Japan] Rotem S., Radzishevsky I., Mor A.; "Physicochemical properties that enhance discriminative antibacterial activity of short dermaseptin derivatives."; Antimicrob. Agents Chemother. 50:2666-2672(2006).
[10]	STRUCTURE BY NMR OF 1-14 OF MUTANT MET-4 AND 14-ALA--ALA-27 DEL. DOI=10.1074/jbc.M513051200; PubMed=16407175 [NCBI, ExPASy, EBI, Israel, Japan] Shalev D.E., Rotem S., Fish A., Mor A.; "Consequences of N-acylation on structure and membrane binding properties of dermaseptin derivative K4-S4-(1-13)."; J. Biol. Chem. 281:9432-9438(2006).

Comments

FUNCTION: Possesses a potent antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, protozoa, and the enveloped herpes simplex virus type 1. Probably acts by disturbing membrane functions with its amphipathic structure. Binds to healthy erythrocytes (this binding is receptor independent), and has strong hemolytic activity. Does not bind to P.falciparum infected erythrocytes, but accumulates within the parasite. Kills the parasite, and only at high concentrations has an hemolytic activity on the host cell.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed by the skin glands.
PHARMACEUTICAL: Derivatives of this peptide may be used as therapeutic agents to treat bacterial infections and malaria, and to prevent infection by herpes simplex virus type 1 and HIV-1.
SIMILARITY: Belongs to the frog skin active peptide (FSAP) family. Dermaseptin subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

3D structure databases

PDB

2DCX; NMR; -; A=1-14.	[ExPASy / RCSB / EBI]
2DD6; NMR; -; A=1-14.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

2DCX; -.
2DD6; -.

ModBase

P80280.

Family and domain databases

BLOCKS

P80280.

Phylogenomic databases

HOVERGEN

P80280; -.

Other

ProtoNet

P80280.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Amphibian defense peptide; Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing; Fungicide; Hemolysis; Pharmaceutical; Secreted.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`PEPTIDE`	`1 27`	`27`	`Dermaseptin-4.`	`PRO_0000043642`
`MUTAGEN`	`4 4`		`M->K: K4-S4-(1-13) selectively disrupts the plasma membrane of the intracellular parasite P.falciparum without harming that of the mammalian host cell; when associated with 14-A--A-27 DEL.`
`MUTAGEN`	`14 27`		`Missing: K4-S4-(1-13) selectively disrupts the plasma membrane of the intracellular parasite P.falciparum without harming that of the mammalian host cell; when associated with K-4.`
`STRAND`	`3 5`	`3`
`HELIX`	`6 10`	`5`

Sequence information

Length: 27 AA [This is the length of the unprocessed precursor]

Molecular weight: 2779 Da [This is the MW of the unprocessed precursor]

CRC64: 43C94D2DC19721A8 [This is a checksum on the sequence]

        10         20 
ALWMTLLKKV LKAAAKALNA VLVGANA

P80280 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools