[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=T213; PubMed=9436993 [NCBI, ExPASy, EBI, Israel, Japan] Wu Q., Sandrock T.M., Turgeon B.G., Yoder O.C., Wirsel S.G., Aist J.R.; "A fungal kinesin required for organelle motility, hyphal growth, and morphogenesis."; Mol. Biol. Cell 9:89-101(1998).

Comments

FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins. Plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. Also involved in postreplication repair of UV-damaged DNA, in N-end rule-dependent protein degradation and in sporulation (By similarity).
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
PATHWAY: Protein modification; protein ubiquitination .
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U86521; AAB47850.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T51931; T51931.

3D structure databases

HSSP

P06104; 1AYZ. [HSSP ENTRY / PDB]

SMR

P78717; 2-151.

ModBase

P78717.

Ontologies

GO:0004842;	Molecular function: ubiquitin-protein ligase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.

PANTHER

PTHR11621; UBQ-conjugat_E2; 1.

Pfam

PF00179; UQ_con; 1.
Pfam graphical view of domain structure.

ProDom

PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00212; UBCc; 1.
SMART graphical view of domain structure.

PROSITE

PS00183; UBIQUITIN_CONJUGAT_1; 1.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chromatin regulator; Cytoplasm; DNA damage; DNA repair; Ligase; Nucleus; Sporulation; Transcription; Transcription regulation; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 151`	`151`	`Ubiquitin-conjugating enzyme E2 2.`	`PRO_0000082534`
`ACT_SITE`	`88 88`		`Glycyl thioester intermediate (By similarity).`

Sequence information

Length: 151 AA [This is the length of the unprocessed precursor]

Molecular weight: 17200 Da [This is the MW of the unprocessed precursor]

CRC64: 157024A4EA568CCF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSTAARRRLM RDFKRMQTDP PAGVSASPVP DNVMTWNAVI IGPADTPFED GTFRLVMQFE 

        70         80         90        100        110        120 
EQYPNKPPQV KFISEMFHPN VYATGELCLD ILQNRWSPTY DVAAVLTSIQ SLLNDPNTGS 

       130        140        150 
PANVEASNLY KDNRKEYTKR VRETVEKSWE D

P78717 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools