ID   PAAG_ECOLI              Reviewed;         262 AA.
AC   P77467; O53015;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   02-SEP-2008, entry version 62.
DE   RecName: Full=Probable enoyl-CoA hydratase paaG;
DE            EC=4.2.1.17;
GN   Name=paaG; Synonyms=ydbT; OrderedLocusNames=b1394, JW1389;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=W / ATCC 11105 / DSM 1900;
RX   MEDLINE=98421522; PubMed=9748275; DOI=10.1074/jbc.273.40.25974;
RA   Ferrandez A., Minambres B., Garcia B., Olivera E.R., Luengo J.M.,
RA   Garcia J.L., Diaz E.;
RT   "Catabolism of phenylacetic acid in Escherichia coli. Characterization
RT   of a new aerobic hybrid pathway.";
RL   J. Biol. Chem. 273:25974-25986(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   MEDLINE=97251357; PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M.,
RA   Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K.,
RA   Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N.,
RA   Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J.,
RA   Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   TRANSCRIPTIONAL REGULATION.
RX   MEDLINE=20229831; PubMed=10766858; DOI=10.1074/jbc.275.16.12214;
RA   Ferrandez A., Garcia J.L., Diaz E.;
RT   "Transcriptional regulation of the divergent paa catabolic operons for
RT   phenylacetic acid degradation in Escherichia coli.";
RL   J. Biol. Chem. 275:12214-12222(2000).
CC   -!- FUNCTION: Could possibly oxidizes fatty acids using specific
CC       components (By similarity).
CC   -!- CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-
CC       CoA + H(2)O.
CC   -!- PATHWAY: Aromatic compound metabolism; phenylacetic acid
CC       degradation.
CC   -!- INTERACTION:
CC       P0AC41:sdhA; NbExp=1; IntAct=EBI-1118484, EBI-371263;
CC   -!- INDUCTION: Activated by cAMP receptor protein (CRP) and
CC       integration host factor (IHF). Inhibited by paaX.
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
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DR   EMBL; X97452; CAA66096.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74476.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15000.1; -; Genomic_DNA.
DR   PIR; E64890; E64890.
DR   RefSeq; AP_002021.1; -.
DR   RefSeq; NP_415912.1; -.
DR   HSSP; P14604; 1MJ3.
DR   IntAct; P77467; -.
DR   GeneID; 946263; -.
DR   GenomeReviews; U00096_GR; b1394.
DR   GenomeReviews; AP009048_GR; JW1389.
DR   KEGG; ecj:JW1389; -.
DR   KEGG; eco:b1394; -.
DR   EchoBASE; EB3504; -.
DR   EcoGene; EG13741; paaG.
DR   HOGENOM; P77467; -.
DR   BioCyc; EcoCyc:G6715-MON; -.
DR   BioCyc; MetaCyc:G6715-MON; -.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR001753; Crotonase_core.
DR   InterPro; IPR011968; PaaB1.
DR   Pfam; PF00378; ECH; 1.
DR   TIGRFAMs; TIGR02280; PaaB1; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Fatty acid metabolism; Lipid metabolism; Lyase.
FT   CHAIN         1    262       Probable enoyl-CoA hydratase paaG.
FT                                /FTId=PRO_0000109331.
FT   VARIANT     121    121       G -> C (in strain: W).
SQ   SEQUENCE   262 AA;  28405 MW;  DD7B95B30D88660C CRC64;
     MMEFILSHVE KGVMTLTLNR PERLNSFNDE MHAQLAECLK QVERDDTIRC LLLTGAGRGF
     CAGQDLNDRN VDPTGPAPDL GMSVERFYNP LVRRLAKLPK PVICAVNGVA AGAGATLALG
     GDIVIAARSA KFVMAFSKLG LIPDCGGTWL LPRVAGRARA MGLALLGNQL SAEQAHEWGM
     IWQVVDDETL ADTAQQLARH LATQPTFGLG LIKQAINSAE TNTLDTQLDL ERDYQRLAGR
     SADYREGVSA FLAKRSPQFT GK
//