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UniProtKB/Swiss-Prot entry P76558


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAO2_ECOLI
Primary accession number P76558
Secondary accession numbers P78200 P78201
Integrated into Swiss-Prot on May 30, 2000
Sequence was last modified on February 1, 1997 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 78)
Name and origin of the protein
Protein name NADP-dependent malic enzyme
Synonyms NADP-ME
EC 1.1.1.40
Gene name
Name: maeB
Synonyms: ypfF
OrderedLocusNames: b2463, JW2447
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/4.2.91; PubMed=9205837 [NCBI, ExPASy, EBI, Israel, Japan]
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.";
DNA Res. 4:91-113(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[4]
CHARACTERIZATION.
STRAIN=W / ATCC 11105 / DSM 1900;
PubMed=36376 [NCBI, ExPASy, EBI, Israel, Japan]
Iwakura M., Hattori J., Arita Y., Tokushige M., Katsuki H.;
"Studies on regulatory functions of malic enzymes. VI. Purification and molecular properties of NADP-linked malic enzyme from Escherichia coli W.";
J. Biochem. 85:1355-1365(1979).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U00096; AAC75516.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA16337.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR F65021; F65021.
RefSeq AP_003048.1; -.
NP_416958.1; -.
3D structure databases
ModBase P76558.
Protein-protein interaction databases
DIP DIP:10141N; -.
IntAct P76558; -.
Enzyme and pathway databases
BioCyc EcoCyc:MALIC-NADP-MON; -.
MetaCyc:MALIC-NADP-MON; -.
Organism-specific databases
EchoBASE EB3945; -.
EcoGene EG14193; maeB.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from UniProtKB).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR012188; ME_PTA.
IPR016040; NAD(P)-bd.
IPR002505; PTA_PTB.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
PF01515; PTA_PTB; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF036684; ME_PTA; 1.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS P76558.
Genome annotation databases
GeneID 946947; -.
GenomeReviews U00096_GR; b2463.
AP009048_GR; JW2447.
KEGG ecj:JW2447; -.
eco:b2463; -.
Phylogenomic databases
HOGENOM P76558; -.
Genome annotation databases
CMR P76558; b2463.
Other
ProtoNet P76558.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; Multifunctional enzyme; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   759  759     NADP-dependent malic enzyme. PRO_0000160242
NP_BIND   76    83  8     NADP (By similarity). 
REGION   1   428  428     Malic enzyme. 
REGION   429   759  331     Phosphate acetyltransferase. 
ACT_SITE   94    94        Proton acceptor (By similarity). 
METAL   136   136        Divalent metal cation (By similarity). 
METAL   137   137        Divalent metal cation (By similarity). 
BINDING   162   162        NAD (By similarity). 
BINDING   288   288        NAD (By similarity). 
Sequence information
Length: 759 AA [This is the length of the unprocessed precursor] Molecular weight: 82417 Da [This is the MW of the unprocessed precursor] CRC64: 9A8B67D9635E25BA [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDDQLKQSAL DFHEFPVPGK IQVSPTKPLA TQRDLALAYS PGVAAPCLEI EKDPLKAYKY 

        70         80         90        100        110        120 
TARGNLVAVI SNGTAVLGLG NIGALAGKPV MEGKGVLFKK FAGIDVFDIE VDELDPDKFI 

       130        140        150        160        170        180 
EVVAALEPTF GGINLEDIKA PECFYIEQKL RERMNIPVFH DDQHGTAIIS TAAILNGLRV 

       190        200        210        220        230        240 
VEKNISDVRM VVSGAGAAAI ACMNLLVALG LQKHNIVVCD SKGVIYQGRE PNMAETKAAY 

       250        260        270        280        290        300 
AVVDDGKRTL DDVIEGADIF LGCSGPKVLT QEMVKKMARA PMILALANPE PEILPPLAKE 

       310        320        330        340        350        360 
VRPDAIICTG RSDYPNQVNN VLCFPFIFRG ALDVGATAIN EEMKLAAVRA IAELAHAEQS 

       370        380        390        400        410        420 
EVVASAYGDQ DLSFGPEYII PKPFDPRLIV KIAPAVAKAA MESGVATRPI ADFDVYIDKL 

       430        440        450        460        470        480 
TEFVYKTNLF MKPIFSQARK APKRVVLPEG EEARVLHATQ ELVTLGLAKP ILIGRPNVIE 

       490        500        510        520        530        540 
MRIQKLGLQI KAGVDFEIVN NESDPRFKEY WTEYFQIMKR RGVTQEQAQR ALISNPTVIG 

       550        560        570        580        590        600 
AIMVQRGEAD AMICGTVGDY HEHFSVVKNV FGYRDGVHTA GAMNALLLPS GNTFIADTYV 

       610        620        630        640        650        660 
NDEPDAEELA EITLMAAETV RRFGIEPRVA LLSHSNFGSS DCPSSSKMRQ ALELVRERAP 

       670        680        690        700        710        720 
ELMIDGEMHG DAALVEAIRN DRMPDSSLKG SANILVMPNM EAARISYNLL RVSSSEGVTV 

       730        740        750 
GPVLMGVAKP VHVLTPIASV RRIVNMVALA VVEAQTQPL 

P76558 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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