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[1]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1093/dnares/3.3.109; PubMed=8905231 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T.,
Sato S.,
Kotani H.,
Tanaka A.,
Asamizu E.,
Nakamura Y.,
Miyajima N.,
Hirosawa M.,
Sugiura M.,
Sasamoto S.,
Kimura T.,
Hosouchi T.,
Matsuno A.,
Muraki A.,
Nakazaki N.,
Naruo K.,
Okumura S.,
Shimpo S.,
Takeuchi C., ![]()
Wada T.,
Watanabe A.,
Yamada M.,
Yasuda M.,
Tabata S.;
"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions.";
DNA Res. 3:109-136(1996).
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[2]
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CHARACTERIZATION OF SPLIT INTEIN.
DOI=10.1073/pnas.95.16.9226; PubMed=9689062 [NCBI, ExPASy, EBI, Israel, Japan]
Wu H.,
Hu Z.,
Liu X.-Q.;
"Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803.";
Proc. Natl. Acad. Sci. U.S.A. 95:9226-9231(1998).
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[3]
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CHARACTERIZATION OF SPLIT INTEIN.
DOI=10.1074/jbc.275.13.9091; PubMed=10734038 [NCBI, ExPASy, EBI, Israel, Japan]
Evans T.C. Jr.,
Martin D.,
Kolly R.,
Panne D.,
Sun L.,
Ghosh I.,
Chen L.,
Benner J.,
Liu X.-Q.,
Xu M.-Q.;
"Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803.";
J. Biol. Chem. 275:9091-9094(2000).
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[4]
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CHARACTERIZATION OF SPLIT INTEIN.
DOI=10.1021/bi001786g; PubMed=11170467 [NCBI, ExPASy, EBI, Israel, Japan]
Martin D.D.,
Xu M.-Q.,
Evans T.C. Jr.;
"Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803.";
Biochemistry 40:1393-1402(2001).
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- FUNCTION: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity).
- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).
- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
- PTM: This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.
- MISCELLANEOUS: The intein is a split intein capable of protein trans-splicing. The N- and C-terminal halves of dnaE are encoded by two separate genes located 745 kb apart in the genome and on opposite DNA strands. The dnaE-N product consists of a N-extein sequence followed by a 123-aa intein sequence (Ssp dnaE1), whereas the dnaE-C product consists of a 36-aa intein sequence (Ssp dnaE2) followed by a C-extein sequence. The N- and C-extein sequences together reconstitute a complete DnaE sequence that is interrupted by the intein sequences inside the beta- and tau-binding domains.
- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE subfamily.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 1355 AA [This is the length of the partial sequence of the unprocessed precursor] |
Molecular weight: 153001 Da [This is the MW of the partial sequence of the unprocessed precursor] |
CRC64: 7C4F61EE4C36EE0F [This is a checksum on the sequence] |
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10 20 30 40 50 60
MSFVGLHIHS DYSLLDGASQ LPALIDRAIE LGMPAIALTD HGVMYGAVEL LKVCRGKPIK
70 80 90 100 110 120
PIIGNEMYVI NGDIEINKRH RRFHQVVLAK NNQGYRNLVK LTTISNLKGI QGSGIFARPC
130 140 150 160 170 180
INKELLEQYK EGLIVTSACL GGEVPQAILK GDLDHARQVA KWYKNLFGDD YYLEIQDHGS
190 200 210 220 230 240
VEDRLVNINL VKIAQELDIK IVATNDSHFI SCNDVEAHDA LLCIQTGKLI TEEKRLRYSG
250 260 270 280 290 300
TEYLKSAEEM HLLFRDHLPD DIIETAIANT LEVADKVEAY SILGEPRIPH YPIPPNHTPE
310 320 330 340 350 360
TYVEDIAWDG LLERLKCRER ADITPVYKER LEYELRMLEK MGFCTYFLVV WDYIKYARDH
370 380 390 400 410 420
GIPVGPGRGS AAGSLVAYCM KITNIDPVHH GLIFERFLNP ERKSMPDVDT DFCIDRRDEM
430 440 450 460 470 480
IEYVTQKYGE DKVAQIITFN RMTSKAVLKD VARVLDIPYA ESDKMAKMIP VSRGKPAKLK
490 500 510 520 530 540
VMISDQTPEP QFKARYDNEP WVKKWIDMAI RIEGTNKTFG VHAAGVVISS EPLDQIVPLQ
550 560 570 580 590 600
KNNDGAVITQ YYMEDVEAMG LLKMDFLGLK NLTTLQRAVE LVAETKGIEL DLDDLPLQER
610 620 630 640 650 660
KALQIRARTG SKKLPDDVKK THKLLEAGDL EGIFQLESQG MKQIVRDLKP SGIEDISSIL
670 680 690 700 710 720
ALYRPGPLDA GLIPIFINRK HGREEISYQH KLLEPILNET YGVLVYQEQI MKMAQDLADY
730 740 750 760 770 780
SLGEADLLRR AMGKKKAEEM QKHRAKFVDG STKHGVPSRI AENLFDQMVK FAEYCLSFGT
790 800 810 820 830 840
EILTVEYGPL PIGKIVSEEI NCSVYSVDPE GRVYTQAIAQ WHDRGEQEVL EYELEDGSVI
850 860 870 880 890 900
RATSDHRFLT TDYQLLAIEE IFARQLDLLT LENIKQTEEA LDNHRLPFPL LDAGTIKMVK
910 920 930 940 950 960
VIGRRSLGVQ RIFDIGLPQD HNFLLANGAI AANCFNKSHS TAYAYVTYQT AYLKANYPVE
970 980 990 1000 1010 1020
YMAALLTASS DSQEKVEKYR ENCQKMGITV EPPDINRSQR HFTPLGEAIL FGLSAVRNLG
1030 1040 1050 1060 1070 1080
EGAIEQIITA RDNSEEKRFK SLADFCTQVD LRVVNRRAIE TLIMAGAFDG VQSNRNQLLH
1090 1100 1110 1120 1130 1140
DLELVIAWAQ KRAKEKETGQ LNIFDSLTAG ESIKAKEAAN NGFEQEPSAP PVAEFSLQEK
1150 1160 1170 1180 1190 1200
LQLEKEHLGF YVSEHPLKSV QRSARLLSPI NLVDLDQYKV RQKVSAVAIL VAVKKIITKK
1210 1220 1230 1240 1250 1260
NGQPMAFLTL EDMSGQSEAV VFPSNYERLQ DVLIEGSQQM IWGKVDRRDD QYQLIVEDLE
1270 1280 1290 1300 1310 1320
PVEEVKMVML DLTPQEIANT STQARLKQIL QSHAPQKEQM KIPVVACINE GPTKHFIRFG
1330 1340 1350
ENYWVNDYGT AVASLQELGF RARLEPLLPQ SQSVG
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P74750 in FASTA format |
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