Phosphopantothenoylcysteine decarboxylase
     (PPCDC)
     (EC 4.1.1.36)
     (CoaC)
Phosphopantothenate--cysteine ligase
     (EC 6.3.2.5)
     (Phosphopantothenoylcysteine synthase)
     (PPC synthetase)
     (PPCS)
     (CoaB)

Gene name

	Name:	coaBC
	Synonyms:	dfp
	OrderedLocusNames:	sll0250

From

Synechocystis sp. (strain PCC 6803)

[TaxID: 1148]

[HAMAP proteome]

Taxonomy

Bacteria; Cyanobacteria; Chroococcales; Synechocystis.

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1093/dnares/3.3.109; PubMed=8905231 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions.";
DNA Res. 3:109-136(1996).

Comments

FUNCTION: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine (By similarity).
CATALYTIC ACTIVITY: N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO₂.
CATALYTIC ACTIVITY: CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + PPi + N-((R)-4'-phosphopantothenoyl)-L-cysteine.
COFACTOR: Binds 1 FMN per subunit (By similarity).
PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis; coenzyme A from pantothenate: step 2/5 .
PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis; coenzyme A from pantothenate: step 3/5 .
SUBUNIT: Homododecamer, the coaB domains form homodimers.
SIMILARITY: In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.
SIMILARITY: In the C-terminal section; belongs to the PPC synthetase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000022; BAA17944.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S75082; S75082.

RefSeq

NP_441264.1; -.

3D structure databases

HSSP

Q9SWE5; 1MVL. [HSSP ENTRY / PDB]

ModBase

P73881.

Enzyme and pathway databases

BioCyc

SSP1148:SLL0250-MON; -.

Ontologies

GO:0010181;	Molecular function: FMN binding (inferred from electronic annotation from InterPro).
GO:0004632;	Molecular function: phosphopantothenate--cysteine ligase activity (inferred from electronic annotation from InterPro).
GO:0004633;	Molecular function: phosphopantothenoylcysteine decarboxylase activity (inferred from electronic annotation from InterPro).
GO:0015937;	Biological process: coenzyme A biosynthetic process (inferred from electronic annotation from InterPro).
GO:0015941;	Biological process: pantothenate catabolic process (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR005252; COABC.
IPR007085; DNA/pantothenate-metab_flavo_C.
IPR003382; Flavoprotein.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.10300; DNA/pantothenate-metab_flavo_C; 1.

Pfam

PF04127; DFP; 1.
PF02441; Flavoprotein; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00521; coaBC_dfp; 1.

ProtoNet

P73881.

Genome annotation databases

GeneID

954606; -.

GenomeReviews

BA000022_GR; sll0250.

KEGG

syn:sll0250; -.

NMPDR

fig|1148.1.peg.1365; -.

Phylogenomic databases

HOGENOM

P73881; -.

Genome annotation databases

CMR

P73881; sll0250.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Decarboxylase; Flavoprotein; FMN; Ligase; Lyase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 402`	`402`	`Coenzyme A biosynthesis bifunctional protein coaBC.`	`PRO_0000232697`
`REGION`	`1 191`	`191`	`Phosphopantothenoylcysteine decarboxylase.`
`REGION`	`192 402`	`211`	`Phosphopantothenate--cysteine ligase.`
`ACT_SITE`	`159 159`		`Proton donor (By similarity).`
`BINDING`	`125 125`		`Substrate (By similarity).`
`BINDING`	`283 283`		`CTP (By similarity).`
`BINDING`	`293 293`		`CTP (By similarity).`
`BINDING`	`342 342`		`CTP (By similarity).`
`BINDING`	`346 346`		`CTP (By similarity).`

Sequence information

Length: 402 AA [This is the length of the unprocessed precursor]

Molecular weight: 43419 Da [This is the MW of the unprocessed precursor]

CRC64: 7E3F73DE185EC97C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVKGKRILIG VGGGIAAYKI CEVVSQLFQQ GAEVRVILTA EAEKFVTPLT FTTLARHPAY 

        70         80         90        100        110        120 
GDADFWQPIH HRPLHIDLGE WADIFLIAPL TAHTLAKLGH GFADDLLSNT VLASSCPILL 

       130        140        150        160        170        180 
APAMNTDMWE QEAVQRNLQQ LLGDRRYHLL APNGGLLACD RRGVGRLAEP AQIMHRLQAL 

       190        200        210        220        230        240 
LFTGGQEDLR GKRILITAGG TQEYLDAVRF IGNPSTGKMG LALAQSAGDR GARVTLIHGP 

       250        260        270        280        290        300 
IGDLPIPMGV ISMAVVNAEQ MEQALAQQAP LADWIVMAAA VADVKPAQTW TGKIAKQDLP 

       310        320        330        340        350        360 
DSLPLAPVPD LIAQVSKQKR PHQLLVGFAA QAGDIVTPAK EKLQRKNLDV IVANPIDQPQ 

       370        380        390        400 
SGFGTDSNQA VIIDRHGSQT AIAPCSKLAM AHRIWQYLLE RI

P73881 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools