ID   LDHD_STAAU              Reviewed;         330 AA.
AC   P72357;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-NOV-2008, entry version 50.
DE   RecName: Full=D-lactate dehydrogenase;
DE            Short=D-LDH;
DE            EC=1.1.1.28;
DE   AltName: Full=D-specific D-2-hydroxyacid dehydrogenase;
GN   Name=ldhD; Synonyms=ddh;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND
RP   FUNCTION.
RC   STRAIN=523k;
RX   MEDLINE=96379887; PubMed=8787900;
RA   Milewski W.M., Boyle-Vavra S., Moreira B., Ebert C.C., Daum R.S.;
RT   "Overproduction of a 37-kilodalton cytoplasmic protein homologous to
RT   NAD(+)-linked D-lactate dehydrogenase associated with vancomycin
RT   resistance in Staphylococcus aureus.";
RL   Antimicrob. Agents Chemother. 40:166-172(1996).
CC   -!- CATALYTIC ACTIVITY: (R)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; U31175; AAB17663.1; -; Genomic_DNA.
DR   HSSP; P26297; 1J49.
DR   GO; GO:0008720; F:D-lactate dehydrogenase activity; IEA:EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006139; D-isomer_2_OHA_DHase.
DR   InterPro; IPR006140; D-isomer_2_OHA_DHase_NAD-bd.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00389; 2-Hacid_dh; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   PROSITE; PS00065; D_2_HYDROXYACID_DH_1; 1.
DR   PROSITE; PS00670; D_2_HYDROXYACID_DH_2; 1.
DR   PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; NAD; Oxidoreductase.
FT   CHAIN         1    330       D-lactate dehydrogenase.
FT                                /FTId=PRO_0000075964.
FT   ACT_SITE    235    235       By similarity.
FT   ACT_SITE    264    264       By similarity.
FT   ACT_SITE    296    296       Proton donor (By similarity).
SQ   SEQUENCE   330 AA;  36682 MW;  A483404D1D240230 CRC64;
     MTKIMFFGTR DYEKEMALNW GKKNNVEVTT SKELLSSATV DQLKDYDGVT TMQFGKLEND
     VYPKLESYGI KQIAQRTAGF DMYDLDLAKK HNIVISNVPS YSPETIAEYS VSIALQLVRR
     FPDIERRVQA HDFTWQAEIM SKPVKNMTVA IIGTGRIGAA TAKIYAGFGA TITAYDAYPN
     KDLDFLTYKD SVKEAIKDAD IISLHVPANK ESYHLFDKAM FDHVKKGAIL VNAARGAVIN
     TPDLIAAVND GTLLGAAIDT YENEAAYFTN DWTNKDIDDK TLLELIEHER ILVTPHIAFF
     SDEAVQNLVE GGLNAALSVI NTGTCETRLN
//