[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=USDA 110spc4; PubMed=8892815 [NCBI, ExPASy, EBI, Israel, Japan] Thoeny-Meyer L., Kuenzler P.; "The Bradyrhizobium japonicum aconitase gene (acnA) is important for free-living growth but not for an effective root nodule symbiosis."; J. Bacteriol. 178:6166-6172(1996).
[2]	ERRATUM. Thoeny-Meyer L., Kuenzler P.; J. Bacteriol. 179:1836-1836(1997).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=USDA 110; DOI=10.1093/dnares/9.6.189; PubMed=12597275 [NCBI, ExPASy, EBI, Israel, Japan] Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.; "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."; DNA Res. 9:189-197(2002).

Comments

CATALYTIC ACTIVITY: Citrate = isocitrate.
COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle .
SUBUNIT: Monomer (By similarity).
SIMILARITY: Belongs to the aconitase/IPM isomerase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U56817; AAC44562.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000040; BAC45731.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_767106.1; -.

3D structure databases

HSSP

P16276; 7ACN. [HSSP ENTRY / SWISS-3DIMAGE / PDB]

ModBase

P70920.

Enzyme and pathway databases

BioCyc

BJAP224911:BLL0466-MON; -.

Ontologies

GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from InterPro).
GO:0003994;	Molecular function: aconitate hydratase activity (inferred from electronic annotation from EC).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006099;	Biological process: tricarboxylic acid cycle (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
IPR001030; Acoase/IPM_deHydtase_lsu_aba.
IPR015937; Aconitase-like_core.
IPR015928; Aconitase/3IPM_dehydase_swvl.
IPR006249; Aconitase/Fe_reg_prot_2.
IPR015934; Aconitase/Fe_reg_prot_2/AcnD.
IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.499.10; Acnase/IPM_dHydase_lsu_aba_1/3; 2.
G3DSA:3.20.19.10; Aconitase/3IPM_dehydase_swvl; 1.
G3DSA:3.40.1060.10; Aconitase/IPMdHydase_lsu_aba_2; 1.

PANTHER

PTHR11670; Aconitase-like_core; 1.
PTHR11670:SF1; Aconitase/Fe_reg_prot_2/AcnD; 1.

Pfam

PF00330; Aconitase; 1.
PF00694; Aconitase_C; 1.
Pfam graphical view of domain structure.

PRINTS

PR00415; ACONITASE.

ProDom

PD000511; Aconitase_N; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR01341; aconitase_1; 1.

PROSITE

PS00450; ACONITASE_1; 1.
PS01244; ACONITASE_2; 1.

ProtoNet

P70920.

Genome annotation databases

GeneID

1052978; -.

GenomeReviews

BA000040_GR; bll0466.

KEGG

bja:bll0466; -.

NMPDR

fig|224911.1.peg.466; -.

Phylogenomic databases

HOGENOM

P70920; -.

Genome annotation databases

CMR

P70920; bll0466.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Iron; Iron-sulfur; Lyase; Metal-binding; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 906`	`906`	`Aconitate hydratase.`	`PRO_0000076656`
`METAL`	`443 443`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`509 509`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`512 512`		`Iron-sulfur (4Fe-4S) (By similarity).`
`CONFLICT`	`596 596`		`W -> C (in Ref. 1; AAC44562).`

Sequence information

Length: 906 AA [This is the length of the unprocessed precursor]

Molecular weight: 98322 Da [This is the MW of the unprocessed precursor]

CRC64: E1A9C4AFA0614104 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTSLDSFKCK KTLKVGAKTY VYYSLPTAEK NGLKGISKLP YSMKVLLENL LRNEDGRSVK 

        70         80         90        100        110        120 
KADIVAVSKW LRKKSLEHEI AFRPARVLMQ DFTGVPAVVD LAAMRNAMQK LGGDAEKINP 

       130        140        150        160        170        180 
LVPVDLVIDH SVIVNFFGDN KAFAKNVTEE YKQNQERYEF LKWGQAAFSN FSVVPPGTGI 

       190        200        210        220        230        240 
CHQVNLEYLS QTVWTKKEKM TVGKKTGTFE VAYPDSLVGT DSHTTMVNGL AVLGWGVGGI 

       250        260        270        280        290        300 
EAEACMLGQP LSMLLPNVVG FKLKGAMKEG VTATDLVLTV TQMLRKLGVV GKFVEFFGPG 

       310        320        330        340        350        360 
LDHLSVADKA TIANMAPEYG ATCGFFPVDA AAIDYLKTSG RAAPRVALVQ AYAKAQGLFR 

       370        380        390        400        410        420 
TAKSADPVFT ETLTLDLADV VPSMAGPKRP EGRIALPSVA EGFSVALANE YKKTEEPAKR 

       430        440        450        460        470        480 
FAVEGKKYEI GHGDVVIAAI TSCTNTSNPS VLIGAGLLAR NAAAKGLKAK PWVKTSLAPG 

       490        500        510        520        530        540 
SQVVAAYLAD SGLQAHLDKV GFNLVGFGCT TCIGNSGPLP EEISKSINDN GIVAAAVLSG 

       550        560        570        580        590        600 
NRNFEGRVSP DVQANYLASP PLVVAHALAG SVTKNLAVEP LGEGKDGKPV YLKDIWPTSK 

       610        620        630        640        650        660 
EINAFMKKFV TASIFKKKYA DVFKGDTNWR KIKTVESETY RWNMSSTYVQ NPPYFEGMKK 

       670        680        690        700        710        720 
EPEPVTDIVE ARILAMFGDK ITTDHISPAG SIKLTSPAGK YLSEHQVRPA DFNQYGTRRG 

       730        740        750        760        770        780 
NHEVMMRGTF ANIRIKNFML KGADGNIPEG GLTKHWPDGE QMSIYDAAMK YQQEQVPLVV 

       790        800        810        820        830        840 
FAGAEYGNGS SRDWAAKGTR LLGVRAVICQ SFERIHRSNL VGMGVLPLTF EEGTSWSSLG 

       850        860        870        880        890        900 
LKGDEKVTLR GLVGDLKPRQ KLTAEIVSGD GSLQRVSLLC RIDTLDELDY YRNGGILHYV 


LRKLAA

P70920 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools