ID   U2D2B_RAT               Reviewed;         147 AA.
AC   P70711;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-NOV-2008, entry version 56.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 D2B;
DE            EC=6.3.2.19;
DE   AltName: Full=Ubiquitin-protein ligase D2B;
DE   AltName: Full=Ubiquitin carrier protein D2B;
DE   AltName: Full=Ubiquitin-conjugating enzyme E2-17 kDa 2B;
DE   AltName: Full=E2(17)KB 2B;
GN   Name=Ube2d2b; Synonyms=Ube2d2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   MEDLINE=96315630; PubMed=8754804;
RA   Wing S.S., Bedard N., Morales C., Hingamp P., Trasler J.;
RT   "A novel rat homolog of the Saccharomyces cerevisiae ubiquitin-
RT   conjugating enzymes UBC4 and UBC5 with distinct biochemical features
RT   is induced during spermatogenesis.";
RL   Mol. Cell. Biol. 16:4064-4072(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. Mediates the selective degradation of short-lived and
CC       abnormal proteins (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP +
CC       diphosphate + protein N-ubiquityllysine.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
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DR   EMBL; U56407; AAC52942.1; -; mRNA.
DR   EMBL; BC078808; AAH78808.1; -; mRNA.
DR   RefSeq; NP_112263.1; -.
DR   UniGene; Rn.113868; -.
DR   HSSP; P15731; 1QCQ.
DR   SMR; P70711; 1-147.
DR   Ensembl; ENSRNOG00000034016; Rattus norvegicus.
DR   GeneID; 79435; -.
DR   KEGG; rno:79435; -.
DR   RGD; 69425; RGD69425.
DR   HOVERGEN; P70711; -.
DR   NextBio; 614807; -.
DR   ArrayExpress; P70711; -.
DR   GermOnline; ENSRNOG00000034016; Rattus norvegicus.
DR   GO; GO:0004842; F:ubiquitin-protein ligase activity; IEA:EC.
DR   GO; GO:0043687; P:post-translational protein modification; IEA:InterPro.
DR   GO; GO:0051246; P:regulation of protein metabolic process; IEA:InterPro.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016135; UBQ-conjugat/RWD-like.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   Gene3D; G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
DR   PANTHER; PTHR11621; UBQ-conjugat_E2; 1.
DR   Pfam; PF00179; UQ_con; 1.
DR   ProDom; PD000461; UBQ_conjugat; 1.
DR   SMART; SM00212; UBCc; 1.
DR   PROSITE; PS00183; UBIQUITIN_CONJUGAT_1; 1.
DR   PROSITE; PS50127; UBIQUITIN_CONJUGAT_2; 1.
PE   2: Evidence at transcript level;
KW   Ligase; Ubl conjugation pathway.
FT   CHAIN         1    147       Ubiquitin-conjugating enzyme E2 D2B.
FT                                /FTId=PRO_0000082469.
FT   ACT_SITE     85     85       Glycyl thioester intermediate (By
FT                                similarity).
SQ   SEQUENCE   147 AA;  16659 MW;  E950720AEC2AB2B2 CRC64;
     MALKRIHKEL NDLAQDPPAQ CSAGPVGEDM FHWQATIMGP NDSPYQGGAF FLTIDFPTEY
     PFKPPKVEFT TRIYHPNVNS NGSICLDILR SQWSPALTIS KVLLSISSLL CDPNPDDPLV
     PEIAQIYKTD RDKYNRTARE WTQKYAM
//