[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Testis; PubMed=8754804 [NCBI, ExPASy, EBI, Israel, Japan] Wing S.S., Bedard N., Morales C., Hingamp P., Trasler J.; "A novel rat homolog of the Saccharomyces cerevisiae ubiquitin-conjugating enzymes UBC4 and UBC5 with distinct biochemical features is induced during spermatogenesis."; Mol. Cell. Biol. 16:4064-4072(1996).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Testis; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins (By similarity).
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
PATHWAY: Protein modification; protein ubiquitination .
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U56407; AAC52942.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC078808; AAH78808.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_112263.1; -.

UniGene

Rn.113868

3D structure databases

HSSP

P15731; 1QCQ. [HSSP ENTRY / PDB]

SMR

P70711; 1-147.

ModBase

P70711.

Organism-specific databases

RGD

69425; RGD69425.

Gene expression databases

ArrayExpress

P70711; -.

GermOnline

ENSRNOG00000034016; Rattus norvegicus.

Ontologies

GO:0004842;	Molecular function: ubiquitin-protein ligase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.

PANTHER

PTHR11621; UBQ-conjugat_E2; 1.

Pfam

PF00179; UQ_con; 1.
Pfam graphical view of domain structure.

ProDom

PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00212; UBCc; 1.
SMART graphical view of domain structure.

PROSITE

PS00183; UBIQUITIN_CONJUGAT_1; 1.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P70711.

Genome annotation databases

Ensembl

ENSRNOG00000034016; Rattus norvegicus. [Contig view]

GeneID

79435; -.

KEGG

rno:79435; -.

Phylogenomic databases

HOVERGEN

P70711; -.

Other

ProtoNet

P70711.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Ligase; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 147`	`147`	`Ubiquitin-conjugating enzyme E2 D2B.`	`PRO_0000082469`
`ACT_SITE`	`85 85`		`Glycyl thioester intermediate (By similarity).`

Sequence information

Length: 147 AA [This is the length of the unprocessed precursor]

Molecular weight: 16659 Da [This is the MW of the unprocessed precursor]

CRC64: E950720AEC2AB2B2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MALKRIHKEL NDLAQDPPAQ CSAGPVGEDM FHWQATIMGP NDSPYQGGAF FLTIDFPTEY 

        70         80         90        100        110        120 
PFKPPKVEFT TRIYHPNVNS NGSICLDILR SQWSPALTIS KVLLSISSLL CDPNPDDPLV 

       130        140 
PEIAQIYKTD RDKYNRTARE WTQKYAM

P70711 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools