[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1006/geno.1996.0531; PubMed=8921381 [NCBI, ExPASy, EBI, Israel, Japan] Seither P., Grummt I.; "Molecular cloning of RPA2, the gene encoding the second largest subunit of mouse RNA polymerase I."; Genomics 37:135-139(1996).

Comments

FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
SUBUNIT: Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits (By similarity).
SUBCELLULAR LOCATION: Nucleus, nucleolus (By similarity).
SIMILARITY: Belongs to the RNA polymerase beta chain family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U58280; AAC52850.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

T42723; T42723.

NP_033112.1; -.

3D structure databases

HSSP

P08518; 1I50. [HSSP ENTRY / PDB]

ModBase

P70700.

PTM databases

PhosphoSite

P70700; -.

Organism-specific databases

MGI

MGI:108014; Rpo1-2.

Gene expression databases

ArrayExpress

P70700; -.

CleanEx

MM_RPA2; -.
MM_RPO1-2; -.

GermOnline

ENSMUSG00000027395; Mus musculus.

Ontologies

GO:0005730;	Cellular component: nucleolus (inferred from direct assay from MGI).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0003899;	Molecular function: DNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from MGI).
GO:0008270;	Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006350;	Biological process: transcription (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR015712; DNA-dir_RNA_pol_su2.
IPR007120; DNA-dir_RNA_pol_su2_6.
IPR007121; RNA_pol_bsu_CS.
IPR007644; RNA_pol_bsu_protrusion.
IPR009674; RNA_pol_Rpa2-specific.
IPR007642; RNA_pol_Rpb2_2.
IPR007645; RNA_pol_Rpb2_3.
IPR007647; RNA_pol_Rpb2_5.
IPR007641; RNA_pol_Rpb2_7.
Graphical view of domain structure.

PANTHER

PTHR20856; RNA_pol_I_sub2; 1.

Pfam

PF06883; RNA_pol_Rpa2_4; 1.
PF04563; RNA_pol_Rpb2_1; 1.
PF04561; RNA_pol_Rpb2_2; 1.
PF04565; RNA_pol_Rpb2_3; 1.
PF04567; RNA_pol_Rpb2_5; 1.
PF00562; RNA_pol_Rpb2_6; 1.
PF04560; RNA_pol_Rpb2_7; 1.
Pfam graphical view of domain structure.

PROSITE

PS01166; RNA_POL_BETA; 1.

ProtoNet

P70700.

Genome annotation databases

Ensembl

ENSMUSG00000027395; Mus musculus. [Contig view]

GeneID

20017; -.

KEGG

mmu:20017; -.

Phylogenomic databases

HOGENOM

P70700; -.

HOVERGEN

P70700; -.

Other

NextBio

297521; -.

SOURCE

Polr1b; Mus musculus.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase; Nucleus; Transcription; Transferase; Zinc; Zinc-finger.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1135`	`1135`	`DNA-directed RNA polymerase I subunit RPA2.`	`PRO_0000048073`
`ZN_FING`	`1070 1101`	`32`	`C4-type (Potential).`

Sequence information

Length: 1135 AA [This is the length of the unprocessed precursor]

Molecular weight: 127976 Da [This is the MW of the unprocessed precursor]

CRC64: 73CAE4BB877608C1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDVDGRWRNL PSGPSLKHLT DPSYGIPPEQ QKAALQDLTR AHVDSFNYAA LEGLSHAVQA 

        70         80         90        100        110        120 
IPPFEFAFKD ERISLTIVDA VISPPSVPKG TICKDLNVYP AECRGRKSTY RGRLTADISW 

       130        140        150        160        170        180 
AVNGVPKGII KQFLGYVPIM VKSKLCNLYN LPPRVLIEHH EEAEEMGGYF IINGIEKVIR 

       190        200        210        220        230        240 
MLIEPRRNFP VAMVRPKWKS RGLGYTQFGV SMRCVREEHS AVNMNLHYVE NGTVMLNFIY 

       250        260        270        280        290        300 
RKELFFLPLG FALKALVSFS DYQIFQELIK GKEEDSFFRN SVSQMLRIVI EEGCHSQKQV 

       310        320        330        340        350        360 
LNYLGECFRV KLSLPDWYPN VEAAEFLLNQ GICIHLQSNT DKFYLRCLMT RKLFALARGE 

       370        380        390        400        410        420 
CMDDNPDSLV NQEVLSPGQL FLMFLKEKME NWLVSIKIVL DKRAQKANVS INNENLMKIF 

       430        440        450        460        470        480 
SMGTELTRPF EYLLATGNLR SKTGLGFLED SGLCVVADKL NFLRYLSHFR CVHRGAAFAK 

       490        500        510        520        530        540 
MRTTTVRRLL PESWGFLCPV HTPDGAPCGL LNHLTAVCEV VTKFGDTASI PALLCGLGVT 

       550        560        570        580        590        600 
GADTAPCRPY SDCYPVLLDG VMVGWVDKDL APEVADTLRR FKVLREKRIP PWMEVALIPM 

       610        620        630        640        650        660 
TGKPSLYPGL FLFTTPCRLV RPVQNLELGR EELIGTMEQL FMNVAIFEDE VFGGISTHQE 

       670        680        690        700        710        720 
LFPHSLLSVI ANFIPFSDHN QSPRNMYQCQ MGKQTMGFPL LTYQNRSDNK LYRLQTPQSP 

       730        740        750        760        770        780 
LVRPCMYDFY DMDNYPIGTN AIVAVISYTG YDMEDAMIVN KASWERGFAH GSVYKSEFID 

       790        800        810        820        830        840 
LSEKFKQGED NLVFGVKPGD PRVMQKLDDD GLPSIGAKLE YGDPYYSYLN LNTGEGFVVY 

       850        860        870        880        890        900 
YKSKENCVVD NIKVCSNDMG SGKFKCICIT VRIPRNPTIG DKFASRHGQK GILSRLWPAE 

       910        920        930        940        950        960 
DMPFTESGMM PDILFNPHGF PSRMTIGMLI ESMAGKSAAL HGLCHDATPF IFSEENSALE 

       970        980        990       1000       1010       1020 
YFGEMLKAAG YNFYGTERLY SGISGMELEA DIFIGVVYYQ RLRHMVSDKF QVRTTGARDK 

      1030       1040       1050       1060       1070       1080 
VTNQPLGGRN VQGGIRFGEM ERDALLAHGT SFLLHDRLFN CSDRSVAHVC VECGSLLSPL 

      1090       1100       1110       1120       1130 
LEKPPPSWSA MRNRKYNCTV CGRSDTIDTV SVPYVFRYFV AELAAMNIKV KLDVI

P70700 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools