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[1]
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NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 44-62; 165-175 AND 283-296.
TISSUE=Spleen;
PubMed=8889821 [NCBI, ExPASy, EBI, Israel, Japan]
Takeda A.,
Masuda Y.,
Yamamoto T.,
Hirabayashi T.,
Nakamura Y.,
Nakaya K.;
"Cloning and analysis of cDNA encoding rat bleomycin hydrolase, a DNA-binding cysteine protease.";
J. Biochem. 120:353-359(1996).
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[2]
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PROTEIN SEQUENCE OF 1-10, ACETYLATION AT MET-1, AND CHARACTERIZATION.
TISSUE=Skin;
PubMed=8907172 [NCBI, ExPASy, EBI, Israel, Japan]
Takeda A.,
Higuchi D.,
Yamamoto T.,
Nakamura Y.,
Masuda Y.,
Hirabayashi T.,
Nakaya K.;
"Purification and characterization of bleomycin hydrolase, which represents a new family of cysteine proteases, from rat skin.";
J. Biochem. 119:29-36(1996).
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- FUNCTION: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity). Binds single-stranded DNA with higher affinity than double-stranded DNA. May play an important role in the metabolism of antibiotics.
- CATALYTIC ACTIVITY: Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.
- BIOPHYSICOCHEMICAL PROPERTIES:
- SUBUNIT: Homohexamer.
- SUBCELLULAR LOCATION: Cytoplasm.
- TISSUE SPECIFICITY: Expressed at relatively higher levels in the stomach, esophagus, spleen, thymus and testis, and at lower levels in the skin, lung and skeletal muscle.
- SIMILARITY: Belongs to the peptidase C1 family [view classification].
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 454 AA [This is the length of the unprocessed precursor] |
Molecular weight: 52323 Da [This is the MW of the unprocessed precursor] |
CRC64: 30BAC4A1A89DC1DC [This is a checksum on the sequence] |
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10 20 30 40 50 60
MNNAGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQGAQ HVFQHVVPQE
70 80 90 100 110 120
GKPVTNQKSS GRCWIFSCLN VMRLPFMKKF NIEEFEFSQS YLFFWDKVER CYFFLNAFVD
130 140 150 160 170 180
TAQKKEPEDG RLVQYLLMNP TNDGGQWDML VNIVEKYGVV PKKCFPESHT TEATRRMNDI
190 200 210 220 230 240
LNHKMREFCI RLRNLVHSGA TKGEISSTQD AMMEEIFRVV CICLGNPPET FTWEYRDKDK
250 260 270 280 290 300
NYHKVGPITP LQFYKEHVKP LFNMEDKICF VNDPRPQHKY NKLYTVDYLS NMVGGRKTLY
310 320 330 340 350 360
NNQPIDFLKK MVAASIRDGE AVWFGCDVGK HFNGKLGLSD MNVYDHELVF GVSLKNMNKA
370 380 390 400 410 420
ERLAFGESLM THAMTFTAVS EKDDQEGAFV KWRVENSWGE DHGHKGYLCM TDEWFSEYVY
430 440 450
EVVVDKKHVP EEVLAVLEQE PIVLPAWDPM GALA
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P70645 in FASTA format |
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