ID   DHB3_MOUSE              Reviewed;         305 AA.
AC   P70385;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-NOV-2008, entry version 57.
DE   RecName: Full=Testosterone 17-beta-dehydrogenase 3;
DE            EC=1.1.1.64;
DE   AltName: Full=Testicular 17-beta-hydroxysteroid dehydrogenase;
DE   AltName: Full=17-beta-HSD 3;
GN   Name=Hsd17b3; Synonyms=Edh17b3;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   MEDLINE=97325842; PubMed=9182854; DOI=10.1016/S0960-0760(96)00165-3;
RA   Sha J.A., Dudley K., Rajapaksha W.R.A.K.J.S., O'Shaughnessy P.J.;
RT   "Sequence of mouse 17beta-hydroxysteroid dehydrogenase type 3 cDNA and
RT   tissue distribution of the type 1 and type 3 isoform mRNAs.";
RL   J. Steroid Biochem. Mol. Biol. 60:19-24(1997).
CC   -!- FUNCTION: Favors the reduction of androstenedione to testosterone.
CC       Uses NADPH while the two other EDH17B enzymes use NADH (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Testosterone + NADP(+) = androst-4-ene-3,17-
CC       dione + NADPH.
CC   -!- PATHWAY: Hormone biosynthesis; testosterone biosynthesis.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family. 17-beta-HSD 3 subfamily.
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DR   EMBL; U66827; AAB06793.1; -; mRNA.
DR   RefSeq; NP_032317.2; -.
DR   UniGene; Mm.5109; -.
DR   Ensembl; ENSMUSG00000033122; Mus musculus.
DR   GeneID; 15487; -.
DR   KEGG; mmu:15487; -.
DR   MGI; MGI:107177; Hsd17b3.
DR   HOGENOM; P70385; -.
DR   HOVERGEN; P70385; -.
DR   NextBio; 288354; -.
DR   ArrayExpress; P70385; -.
DR   CleanEx; MM_HSD17B3; -.
DR   GermOnline; ENSMUSG00000033122; Mus musculus.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0047045; F:testosterone 17-beta-dehydrogenase (NADP+) ...; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DHase_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DHase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   2: Evidence at transcript level;
KW   Lipid synthesis; NADP; Oxidoreductase; Steroid biosynthesis.
FT   CHAIN         1    305       Testosterone 17-beta-dehydrogenase 3.
FT                                /FTId=PRO_0000054574.
FT   NP_BIND      44     73       NADP (By similarity).
FT   ACT_SITE    194    194       Proton acceptor (By similarity).
FT   BINDING     181    181       Substrate (By similarity).
SQ   SEQUENCE   305 AA;  34271 MW;  183860C85213EB61 CRC64;
     MEKLFIAAGL FVGLVCLVKC MRFSQHLFLR FCKALPSSFL RSMGQWAVIT GAGDGIGKAY
     SFELARHGLN VVLISRTLEK LQTIAEEIER TTGSGVKIVQ ADFTREDIYD HIKEHLEGLE
     NGILVNNVGM LPSFFPSHFL SSSGESQNLI HCNITSVVKM TQLVLKHMES RRKGLILNIS
     SGAALRPWPL YSLYSASKAF VYTFSKALSV EYRDKGIIIQ VLTPYSISTP MTKYLNNKMT
     KTADEFVKES LKYVTIGAES CGCLAHEIIA IILNRIPSRI FYSSTAQRFL LTRYSDYLKR
     NISNR
//