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UniProtKB/Swiss-Prot entry P70343

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CASP4_MOUSE
Primary accession number P70343
Secondary accession number O08735
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on February 1, 1997 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 71)
Name and origin of the protein
Protein name Caspase-4 [Precursor]
Synonyms CASP-4
EC 3.4.22.64
Caspase-11
CASP-11
ICH-3 protease
Contains Caspase-4 subunit p10
Caspase-4 subunit p20
Gene name
Name: Casp4
Synonyms: Casp11, Caspl, Ich3
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=C57BL/6 X CBA;
TISSUE=Thymus;
DOI=10.1074/jbc.271.34.20580; PubMed=8702803 [NCBI, ExPASy, EBI, Israel, Japan]
Wang S., Miura M., Jung Y.-K., Zhu H., Gagliardini V., Shi L., Greenberg A.H., Yuan J.;
"Identification and characterization of Ich-3, a member of the interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE.";
J. Biol. Chem. 271:20580-20587(1996).
[2]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=C3H/An;
DOI=10.1016/S0014-5793(97)00026-4; PubMed=9038361 [NCBI, ExPASy, EBI, Israel, Japan]
van de Craen M., Vandenabeele P., Declercq W., van den Brande I., van Loo G., Molemans F., Schotte P., van Criekinge W., Beyaert R., Fiers W.;
"Characterization of seven murine caspase family members.";
FEBS Lett. 403:61-69(1997).
Comments
  • FUNCTION: Involved in the activation cascade of caspases responsible for apoptosis execution. Promotes IL-1 beta processing by ICE, so may also have a role in inflammatory responses.
  • CATALYTIC ACTIVITY: Strict requirement for Asp at the P1 position and has a preferred cleavage sequence of (Ile/Leu/Val/Phe)-Gly-His-Asp-|-.
  • SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit.
  • TISSUE SPECIFICITY: Mostly expressed in lung and spleen; weaker in heart and lung; little in liver, skeletal muscle, kidney and testis. Not found in the brain.
  • INDUCTION: Activity increased 30-fold by endotoxins (LPS).
  • PTM: The two subunits are derived from the precursor sequence by a autocatalytic mechanism.
  • SIMILARITY: Belongs to the peptidase C14 family [view classification].
  • SIMILARITY: Contains 1 CARD domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U59463; AAB09469.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y13089; CAA73531.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
UniGene Mm.1569
3D structure databases
HSSP P29466; 1ICE. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
ModBase P70343.
Protein family/group databases
MEROPS C14.012; -.
PTM databases
PhosphoSite P70343; -.
Organism-specific databases
MGI MGI:107700; Casp4.
Gene expression databases
ArrayExpress P70343; -.
CleanEx MM_CASP4; -.
GermOnline ENSMUSG00000033538; Mus musculus.
Ontologies
GO
GO:0005622; Cellular component: intracellular (inferred from electronic annotation from InterPro).
GO:0004197; Molecular function: cysteine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0005515; Molecular function: protein binding (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001315; CARD.
IPR017350; Caspase_IL-1_beta.
IPR011029; DEATH_like.
IPR011600; Pept_C14_cat.
IPR001309; Pept_C14_ICE_p20.
IPR016129; Pept_C14_ICE_p20_AS.
IPR002138; Pept_C14_p10.
IPR002398; Pept_C14_p45.
IPR015917; Pept_C14_p45_core.
Graphical view of domain structure.
Gene3D G3DSA:1.10.533.10; DEATH_like; 1.
PANTHER PTHR10454; Pept_C14_p45; 1.
Pfam PF00619; CARD; 1.
PF00656; Peptidase_C14; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF038001; Caspase_ICE; 1.
PRINTS PR00376; IL1BCENZYME.
SMART SM00114; CARD; 1.
SM00115; CASc; 1.
SMART graphical view of domain structure.
PROSITE PS50209; CARD; 1.
PS01122; CASPASE_CYS; 1.
PS01121; CASPASE_HIS; 1.
PS50207; CASPASE_P10; 1.
PS50208; CASPASE_P20; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P70343.
Genome annotation databases
Ensembl ENSMUSG00000033538; Mus musculus. [Contig view]
Phylogenomic databases
HOGENOM P70343; -.
HOVERGEN P70343; -.
Other
SOURCE Casp4; Mus musculus.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Apoptosis; Hydrolase; Phosphoprotein; Protease; Thiol protease; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
PROPEP   1    80  80     Potential. PRO_0000004600
CHAIN   81   266  186     Caspase-4 subunit p20. PRO_0000004601
PROPEP   267   285  19     Potential. PRO_0000004602
CHAIN   286   373  88     Caspase-4 subunit p10. PRO_0000004603
DOMAIN   1    91  91     CARD. 
ACT_SITE   206   206        By similarity. 
ACT_SITE   254   254         
MOD_RES   83    83        Phosphoserine (By similarity). 
MUTAGEN   254   254        C->G: No cell death. 
CONFLICT   152   152        K -> N (in Ref. 2; CAA73531). 
Sequence information
Length: 373 AA [This is the length of the unprocessed precursor] Molecular weight: 42756 Da [This is the MW of the unprocessed precursor] CRC64: 574B0EC01804E0B4 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAENKHPDKP LKVLEQLGKE VLTEYLEKLV QSNVLKLKEE DKQKFNNAER SDKRWVFVDA 

        70         80         90        100        110        120 
MKKKHSKVGE MLLQTFFSVD PGSHHGEANL EMEEPEESLN TLKLCSPEEF TRLCREKTQE 

       130        140        150        160        170        180 
IYPIKEANGR TRKALIICNT EFKHLSLRYG AKFDIIGMKG LLEDLGYDVV VKEELTAEGM 

       190        200        210        220        230        240 
ESEMKDFAAL SEHQTSDSTF LVLMSHGTLH GICGTMHSEK TPDVLQYDTI YQIFNNCHCP 

       250        260        270        280        290        300 
GLRDKPKVII VQACRGGNSG EMWIRESSKP QLCRGVDLPR NMEADAVKLS HVEKDFIAFY 

       310        320        330        340        350        360 
STTPHHLSYR DKTGGSYFIT RLISCFRKHA CSCHLFDIFL KVQQSFEKAS IHSQMPTIDR 

       370 
ATLTRYFYLF PGN
P70343 in FASTA format

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