Mannitol permease IIC component
     (PTS system mannitol-specific EIIC component)
Mannitol-specific phosphotransferase enzyme IIB component
     (EC 2.7.1.69)
     (PTS system mannitol-specific EIIB component)

Gene name

	Name:	cmtA
	OrderedLocusNames:	b2933, JW2900

From

Escherichia coli (strain K12)

[TaxID: 83333]

[HAMAP proteome]

Taxonomy

Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.

Protein existence

1: Evidence at protein level;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-446. STRAIN=K12; DOI=10.1016/0304-4165(93)90103-F; PubMed=8353127 [NCBI, ExPASy, EBI, Israel, Japan] Sprenger G.A.; "Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various Gram-positive bacteria."; Biochim. Biophys. Acta 1158:103-106(1993).
[2]	PRESENCE OF VECTOR SEQUENCE CONTAMINATION IN SEQUENCE DESCRIBED IN PUBMED:8353127. Rudd K.E.; Unpublished observations (JUN-1993).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / MG1655 / ATCC 47076; DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan] Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.; "The complete genome sequence of Escherichia coli K-12."; Science 277:1453-1474(1997).
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan] Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."; Mol. Syst. Biol. 2:E1-E5(2006).
[5]	SUBCELLULAR LOCATION. STRAIN=K12 / MG1655 / ATCC 47076; DOI=10.1126/science.1109730; PubMed=15919996 [NCBI, ExPASy, EBI, Israel, Japan] Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.; "Global topology analysis of the Escherichia coli inner membrane proteome."; Science 308:1321-1323(2005).

Comments

FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport (By similarity).
CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
INTERACTION:
P02942:tsr; NbExp=1; IntAct=EBI-1131045, EBI-560870;
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
DOMAIN: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
SIMILARITY: Contains 1 PTS EIIB type-2 domain.
SIMILARITY: Contains 1 PTS EIIC type-2 domain.
SEQUENCE CAUTION:
- Sequence=CAA51229.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Vector contamination at the C-terminus

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X72677; CAA51229.1; ALT_TERM; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U28377; AAA69100.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC75970.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE76996.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

D65078; S36123.

RefSeq

AP_003490.1; -.
NP_417408.1; -.

3D structure databases

ModBase

P69826.

Protein-protein interaction databases

IntAct

P69826; -.

Enzyme and pathway databases

BioCyc

EcoCyc:CMTA-MON; -.

Organism-specific databases

EchoBASE

EB1740; -.

EcoGene

EG11792; cmtA.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0016301;	Molecular function: kinase activity (inferred from electronic annotation from UniProtKB-KW).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0008982;	Molecular function: protein-N(PI)-phosphohistidine-sugar phosphotransferase activity (inferred from electronic annotation from InterPro).
GO:0005351;	Molecular function: sugar:hydrogen symporter activity (inferred from electronic annotation from InterPro).
GO:0009401;	Biological process: phosphoenolpyruvate-dependent sugar phosphotransferase system (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR013011; PTS_EIIB_2.
IPR003352; PTS_EIIC.
IPR013014; PTS_EIIC_2.
IPR003501; PTS_IIB_lac.
IPR004718; PTS_IIC_mtl.
Graphical view of domain structure.

Pfam

PF02378; PTS_EIIC; 1.
PF02302; PTS_IIB; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00851; mtlA; 1.

PROSITE

PS51099; PTS_EIIB_TYPE_2; 1.
PS51104; PTS_EIIC_TYPE_2; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P69826.

Genome annotation databases

GeneID

945256; -.

GenomeReviews

U00096_GR; b2933.
AP009048_GR; JW2900.

KEGG

ecj:JW2900; -.
eco:b2933; -.

Phylogenomic databases

HOGENOM

P69826; -.

Genome annotation databases

CMR

P69826; b2933.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell inner membrane; Cell membrane; Complete proteome; Kinase; Membrane; Phosphotransferase system; Sugar transport; Transferase; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 462`	`462`	`PTS system mannitol-specific cryptic EIICB component.`	`PRO_0000186679`
`TOPO_DOM`	`1 25`	`25`	`Cytoplasmic (Potential).`
`TRANSMEM`	`26 45`	`20`	`Probable.`
`TOPO_DOM`	`46 51`	`6`	`Periplasmic (Potential).`
`TRANSMEM`	`52 70`	`19`	`Probable.`
`TOPO_DOM`	`71 135`	`65`	`Cytoplasmic (Potential).`
`TRANSMEM`	`136 155`	`20`	`Probable.`
`TOPO_DOM`	`156 166`	`11`	`Periplasmic (Potential).`
`TRANSMEM`	`167 185`	`19`	`Probable.`
`TOPO_DOM`	`186 273`	`88`	`Cytoplasmic (Potential).`
`TRANSMEM`	`274 294`	`21`	`Probable.`
`TOPO_DOM`	`295 316`	`22`	`Periplasmic (Potential).`
`TRANSMEM`	`317 336`	`20`	`Probable.`
`TOPO_DOM`	`337 462`	`126`	`Cytoplasmic (Potential).`
`DOMAIN`	`13 344`	`332`	`PTS EIIC type-2.`
`DOMAIN`	`371 461`	`91`	`PTS EIIB type-2.`
`ACT_SITE`	`377 377`		`Phosphocysteine intermediate; for EIIB activity (By similarity).`
`CONFLICT`	`274 275`		`MI -> IEF (in Ref. 1; CAA51229).`
`CONFLICT`	`415 435`		`DADIVVTHASLEGRVKRVTDK -> GCRISGRLHPWPRSWKGAVETGVRN (in Ref. 1; CAA51229).`

Sequence information

Length: 462 AA [This is the length of the unprocessed precursor]

Molecular weight: 48971 Da [This is the MW of the unprocessed precursor]

CRC64: 83F2D203D9E5A4C4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MENKSARAKV QAFGGFLTAM VIPNIGAFIA WGFITALFIP TGWLPNEHFA KIVGPMITYL 

        70         80         90        100        110        120 
LPVMIGSTGG HLVGGKRGAV MGGIGTIGVI VGAEIPMFLG SMIMGPLGGL VIKYVDKALE 

       130        140        150        160        170        180 
KRIPAGFEMV INNFSLGIAG MLLCLLGFEV IGPAVLIANT FVKECIEALV HAGYLPLLSV 

       190        200        210        220        230        240 
INEPAKVLFL NNAIDQGVYY PLGMQQASVN GKSIFFMVAS NPGPGLGLLL AFTLFGKGMS 

       250        260        270        280        290        300 
KRSAPGAMII HFLGGIHELY FPYVLMKPLT IIAMIAGGMS GTWMFNLLDG GLVAGPSPGS 

       310        320        330        340        350        360 
IFAYLALTPK GSFLATIAGV TVGTLVSFAI TSLILKMEKT VETESEDEFA QSANAVKAMK 

       370        380        390        400        410        420 
QEGAFSLSRV KRIAFVCDAG MGSSAMGATT FRKRLEKAGL AIEVKHYAIE NVPADADIVV 

       430        440        450        460 
THASLEGRVK RVTDKPLILI NNYIGDPKLD TLFNQLTAEH KH

P69826 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools