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UniProtKB/Swiss-Prot entry P69818


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PTFB2_ECO57
Primary accession number P69818
Secondary accession number P32673
Integrated into Swiss-Prot on May 10, 2005
Sequence was last modified on May 10, 2005 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 24)
Name and origin of the protein
Protein name Fructose-like phosphotransferase enzyme IIB component 2
Synonyms EC 2.7.1.69
PTS system fructose-like EIIB component 2
Gene name
Name: frwB
OrderedLocusNames: Z5506, ECs4879
From
Escherichia coli O157:H7 [TaxID: 83334] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
DOI=10.1038/35054089; PubMed=11206551 [NCBI, ExPASy, EBI, Israel, Japan]
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.;
"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
Nature 409:529-533(2001).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
DOI=10.1093/dnares/8.1.11; PubMed=11258796 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S., Shiba T., Hattori M., Shinagawa H.;
"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12.";
DNA Res. 8:11-22(2001).
Comments
  • FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (By similarity).
  • CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
  • SUBCELLULAR LOCATION: Cytoplasm (Potential).
  • DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
  • SIMILARITY: Contains 1 PTS EIIB type-2 domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE005174; AAG59152.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000007; BAB38302.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D86086; D86086.
G91238; G91238.
RefSeq NP_290587.1; -.
NP_312906.1; -.
3D structure databases
ModBase P69818.
Enzyme and pathway databases
BioCyc ECOL83334:ECS4879-MON; -.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0016020; Cellular component: membrane (inferred from electronic annotation from InterPro).
GO:0016301; Molecular function: kinase activity (inferred from electronic annotation from UniProtKB-KW).
GO:0008982; Molecular function: protein-N(PI)-phosphohistidine-sugar phosphotransferase activity (inferred from electronic annotation from InterPro).
GO:0005351; Molecular function: sugar:hydrogen symporter activity (inferred from electronic annotation from InterPro).
GO:0009401; Biological process: phosphoenolpyruvate-dependent sugar phosphotransferase system (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR013011; PTS_EIIB_2.
IPR003353; PTS_IIB_fruc.
Graphical view of domain structure.
Pfam PF02379; PTS_IIB_fruc; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00829; FRU; 1.
PROSITE PS51099; PTS_EIIB_TYPE_2; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P69818.
Genome annotation databases
GeneID 915006; -.
960182; -.
GenomeReviews BA000007_GR; ECs4879.
AE005174_GR; Z5506.
KEGG ece:Z5506; -.
ecs:ECs4879; -.
Phylogenomic databases
HOGENOM P69818; -.
Other
LinkHub P69818; -.
Genome annotation databases
CMR P69818; Z5506.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Kinase; Phosphotransferase system; Sugar transport; Transferase; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom  To Length Description FTId
CHAIN   1   106  106     Fructose-like phosphotransferase enzyme IIB component 2. PRO_0000186503
DOMAIN   1   103  103     PTS EIIB type-2. 
ACT_SITE   10    10        Phosphocysteine intermediate (By similarity). 
Sequence information
Length: 106 AA [This is the length of the unprocessed precursor] Molecular weight: 11248 Da [This is the MW of the unprocessed precursor] CRC64: F712189F18489D3D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTKIIAVTAC PSGVAHTYMA AEALESAAKA KGWEVKVETQ GSIGLENELT AEDVASADMV 

        70         80         90        100 
ILTKDIGIKF EERFAGKTIV RVNISDAVKR ADAIMSKIEA HLAQTA 

P69818 in FASTA format

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