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UniProtKB/Swiss-Prot entry P69743

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MBHT_ECO57
Primary accession number P69743
Secondary accession number Q46847
Integrated into Swiss-Prot on April 26, 2005
Sequence was last modified on April 26, 2005 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 31)
Name and origin of the protein
Protein name Hydrogenase-2 small chain [Precursor]
Synonyms HYD2
EC 1.12.99.6
Membrane-bound hydrogenase 2 small subunit
NiFe hydrogenase
Gene name
Name: hybO
OrderedLocusNames: Z4351, ECs3882
From
Escherichia coli O157:H7 [TaxID: 83334] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
DOI=10.1038/35054089; PubMed=11206551 [NCBI, ExPASy, EBI, Israel, Japan]
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.;
"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
Nature 409:529-533(2001).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
DOI=10.1093/dnares/8.1.11; PubMed=11258796 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S., Shiba T., Hattori M., Shinagawa H.;
"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12.";
DNA Res. 8:11-22(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE005174; AAG58134.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000007; BAB37305.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B85959; B85959.
B91114; B91114.
RefSeq NP_289575.1; -.
NP_311909.1; -.
3D structure databases
HSSP P18187; 1FRF. [HSSP ENTRY / PDB]
ModBase P69743.
Enzyme and pathway databases
BioCyc ECOL83334:ECS3882-MON; -.
Ontologies
GO
GO:0009375; Cellular component: ferredoxin hydrogenase complex (inferred from electronic annotation from InterPro).
GO:0042597; Cellular component: periplasmic space (inferred from electronic annotation from UniProtKB-KW).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0051538; Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008901; Molecular function: ferredoxin hydrogenase activity (inferred from electronic annotation from InterPro).
GO:0033748; Molecular function: hydrogenase (acceptor) activity (inferred from electronic annotation from EC).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008137; Molecular function: NADH dehydrogenase (ubiquinone) activity (inferred from electronic annotation from InterPro).
GO:0006120; Biological process: mitochondrial electron transport, NADH to ubiquinone (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001821; NiFe_hyd_ssu.
IPR013634; NiFe_hyd_ssu_N.
IPR006137; OxRdtase_q6.
IPR006311; Tat.
Graphical view of domain structure.
Pfam PF08425; NiFe_dehyd_N; 1.
PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000310; NiFe_hyd_ssu; 1.
PRINTS PR00614; NIHGNASESMLL.
TIGRFAMs TIGR00391; hydA; 1.
TIGR01409; TAT_signal_seq; 1.
PROSITE PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P69743.
Genome annotation databases
GeneID 916289; -.
958478; -.
GenomeReviews BA000007_GR; ECs3882.
AE005174_GR; Z4351.
KEGG ece:Z4351; -.
ecs:ECs3882; -.
Phylogenomic databases
HOGENOM P69743; -.
Genome annotation databases
CMR P69743; Z4351.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3Fe-4S; 4Fe-4S; Cell membrane; Complete proteome; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Periplasm; Signal.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    37  37     Tat-type signal (Potential). 
CHAIN   38   372  335     Hydrogenase-2 small chain. PRO_0000013432
METAL   59    59        Iron-sulfur 1 (4Fe-4S) (By similarity). 
METAL   62    62        Iron-sulfur 1 (4Fe-4S) (By similarity). 
METAL   157   157        Iron-sulfur 1 (4Fe-4S) (By similarity). 
METAL   191   191        Iron-sulfur 1 (4Fe-4S) (By similarity). 
METAL   229   229        Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity). 
METAL   232   232        Iron-sulfur 2 (4Fe-4S) (By similarity). 
METAL   257   257        Iron-sulfur 2 (4Fe-4S) (By similarity). 
METAL   263   263        Iron-sulfur 2 (4Fe-4S) (By similarity). 
METAL   272   272        Iron-sulfur 3 (3Fe-4S) (By similarity). 
METAL   292   292        Iron-sulfur 3 (3Fe-4S) (By similarity). 
METAL   295   295        Iron-sulfur 3 (3Fe-4S) (By similarity). 
Sequence information
Length: 372 AA [This is the length of the unprocessed precursor] Molecular weight: 39652 Da [This is the MW of the unprocessed precursor] CRC64: C5821096BBAF9C66 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTGDNTLIHS HGINRRDFMK LCAALAATMG LSSKAAAEMA ESVTNPQRPP VIWIGAQECT 

        70         80         90        100        110        120 
GCTESLLRAT HPTVENLVLE TISLEYHEVL SAAFGHQVEE NKHNALEKYK GQYVLVVDGS 

       130        140        150        160        170        180 
IPLKDNGIYC MVAGEPIVDH IRKAAEGAAA IIAIGSCSAW GGVAAAGVNP TGAVSLQEVL 

       190        200        210        220        230        240 
PGKTVINIPG CPPNPHNFLA TVAHIITYGK PPKLDDKNRP TFAYGRLIHE HCERRPHFDA 

       250        260        270        280        290        300 
GRFAKEFGDE GHREGWCLYH LGCKGPETYG NCSTLQFCDV GGVWPVAIGH PCYGCNEEGI 

       310        320        330        340        350        360 
GFHKGIHQLA NVENQTPRSQ KPDVNAKEGG NVSAGAIGLL GGVVGLVAGV SVMAVRELGR 

       370 
QQKKDNADSR GE
P69743 in FASTA format

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