ID   HSD_MYCTU               Reviewed;         260 AA.
AC   P69167; Q10855;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   04-NOV-2008, entry version 28.
DE   RecName: Full=3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase;
DE            EC=1.1.1.53;
GN   Name=fabG3; OrderedLocusNames=Rv2002, MT2058; ORFNames=MTCY39.16c;
OS   Mycobacterium tuberculosis.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=1773;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   MEDLINE=98295987; PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M.,
RA   Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III,
RA   Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T.,
RA   Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S.,
RA   Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A.,
RA   Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R.,
RA   Sulston J.E., Taylor K., Whitehead S., Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the
RT   complete genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   MEDLINE=22206494; PubMed=12218036;
RX   DOI=10.1128/JB.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H.,
RA   Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D.,
RA   Salzberg S.L., Delcher A., Utterback T.R., Weidman J.F., Khouri H.M.,
RA   Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C.,
RA   Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANTS THR-6; MET-47 AND
RP   LYS-69.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=12524453; DOI=10.1073/pnas.0137017100;
RA   Yang J.K., Park M.S., Waldo G.S., Suh S.W.;
RT   "Directed evolution approach to a structural genomics project: Rv2002
RT   from Mycobacterium tuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:455-460(2003).
CC   -!- CATALYTIC ACTIVITY: Androstan-3-alpha,17-beta-diol + NAD(+) = 17-
CC       beta-hydroxyandrostan-3-one + NADH.
CC   -!- PATHWAY: Lipid metabolism; C21-steroid hormone metabolism.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; BX842578; CAA98414.1; -; Genomic_DNA.
DR   EMBL; AE000516; AAK46335.1; -; Genomic_DNA.
DR   PIR; H70758; H70758.
DR   RefSeq; NP_216518.1; -.
DR   RefSeq; NP_336521.1; -.
DR   PDB; 1NFF; X-ray; 1.80 A; A/B=1-260.
DR   PDB; 1NFQ; X-ray; 2.40 A; A/B/C/D=1-260.
DR   PDB; 1NFR; X-ray; 2.10 A; A/B/C/D=1-260.
DR   PDBsum; 1NFF; -.
DR   PDBsum; 1NFQ; -.
DR   PDBsum; 1NFR; -.
DR   GeneID; 888857; -.
DR   GeneID; 923379; -.
DR   GenomeReviews; AE000516_GR; MT2058.
DR   GenomeReviews; AL123456_GR; Rv2002.
DR   KEGG; mtc:MT2058; -.
DR   KEGG; mtu:Rv2002; -.
DR   TIGR; MT2058; -.
DR   TubercuList; Rv2002; -.
DR   HOGENOM; P69167; -.
DR   GO; GO:0047044; F:3-alpha(or 20-beta)-hydroxysteroid dehydrog...; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DHase_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DHase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Lipid metabolism; NAD;
KW   Oxidoreductase; Steroid metabolism.
FT   CHAIN         1    260       3-alpha-(or 20-beta)-hydroxysteroid
FT                                dehydrogenase.
FT                                /FTId=PRO_0000054712.
FT   NP_BIND      11     35       NAD (By similarity).
FT   ACT_SITE    153    153       Proton acceptor.
FT   BINDING     140    140       Substrate.
FT   CONFLICT    174    174       S -> G (in Ref. 2; AAK46335).
FT   TURN          4      7
FT   STRAND        9     13
FT   TURN         14     16
FT   HELIX        18     29
FT   STRAND       33     39
FT   HELIX        41     50
FT   HELIX        52     54
FT   STRAND       55     59
FT   HELIX        65     79
FT   STRAND       84     87
FT   TURN         97     99
FT   HELIX       102    112
FT   HELIX       114    130
FT   STRAND      133    138
FT   HELIX       141    143
FT   HELIX       151    171
FT   HELIX       172    174
FT   STRAND      176    183
FT   HELIX       189    191
FT   STRAND      202    205
FT   HELIX       209    220
FT   HELIX       222    224
FT   STRAND      231    235
FT   HELIX       238    240
SQ   SEQUENCE   260 AA;  27030 MW;  0935A14ED36220B7 CRC64;
     MSGRLIGKVA LVSGGARGMG ASHVRAMVAE GAKVVFGDIL DEEGKAVAAE LADAARYVHL
     DVTQPAQWTA AVDTAVTAFG GLHVLVNNAG ILNIGTIEDY ALTEWQRILD VNLTGVFLGI
     RAVVKPMKEA GRGSIINISS IEGLAGTVAC HGYTATKFAV RGLTKSTALE LGPSGIRVNS
     IHPGLVKTPM TDWVPEDIFQ TALGRAAEPV EVSNLVVYLA SDESSYSTGA EFVVDGGTVA
     GLAHNDFGAV EVSSQPEWVT
//