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UniProtKB/Swiss-Prot entry P68736


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NPRE_BACSU
Primary accession number P68736
Secondary accession numbers P06142 P25268
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on August 1, 1988 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 35)
Name and origin of the protein
Protein name Bacillolysin [Precursor]
Synonyms EC 3.4.24.28
Neutral protease
MCP 76
Gene name
Name: nprE
OrderedLocusNames: BSU14700
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=6090407 [NCBI, ExPASy, EBI, Israel, Japan]
Yang M.Y., Ferrari E., Henner D.J.;
"Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.";
J. Bacteriol. 160:15-21(1984).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=8969500 [NCBI, ExPASy, EBI, Israel, Japan]
Winters P., Caldwell R.M., Enfield L., Ferrari E.;
"The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area.";
Microbiology 142:3033-3037(1996).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=NS15-4;
Lee S., Yoon K., Nam H., Chae K.;
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-158.
STRAIN=168;
Purnell B., Presecan E., Glaser P., Richou A., Danchin A., Goffeau A.;
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
K01985; AAA22627.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF012285; AAC24942.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U30932; AAA82609.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99111; CAB13343.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z97025; CAB09705.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A25414; HYBS.
JQ2129; JQ2129.
RefSeq NP_389353.1; -.
3D structure databases
HSSP P81177; 1BQB. [HSSP ENTRY / PDB]
ModBase P68736.
Protein family/group databases
MEROPS M04.014; -.
Enzyme and pathway databases
BioCyc BSUB224308:BSU1472-MON; -.
Organism-specific databases
SubtiList BG10448; nprE. [Micado]
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred from electronic annotation from InterPro).
GO:0005509; Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004222; Molecular function: metalloendopeptidase activity (inferred from electronic annotation from InterPro).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from InterPro).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR005075; Pept_M4_propep_PepSY.
IPR006025; Pept_M_Zn_BS.
IPR013856; Peptidase_M4.
IPR001570; Peptidase_M4_C.
IPR011096; Propep_M4_M36.
Graphical view of domain structure.
Gene3D G3DSA:3.10.170.10; Peptidase_M4; 1.
G3DSA:1.10.390.10; Peptidase_M4/M36; 1.
Pfam PF07504; FTP; 1.
PF03413; PepSY; 1.
PF01447; Peptidase_M4; 1.
PF02868; Peptidase_M4_C; 1.
Pfam graphical view of domain structure.
PRINTS PR00730; THERMOLYSIN.
PROSITE PS00142; ZINC_PROTEASE; 1.
ProtoNet P68736.
Genome annotation databases
GeneID 935981; -.
GenomeReviews AL009126_GR; BSU14700.
KEGG bsu:BSU14700; -.
NMPDR fig|224308.1.peg.1472; -.
Phylogenomic databases
HOGENOM P68736; -.
Genome annotation databases
CMR P68736; BSU14700.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Complete proteome; Hydrolase; Metal-binding; Metalloprotease; Protease; Secreted; Signal; Zinc; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    27  27     Potential. 
PROPEP   28   221  194     Activation peptide (By similarity). PRO_0000028606
CHAIN   222   521  300     Bacillolysin. PRO_0000028607
ACT_SITE   365   365        By similarity. 
ACT_SITE   449   449        Proton donor (By similarity). 
METAL   360   360        Calcium 1 (Potential). 
METAL   364   364        Zinc; catalytic (By similarity). 
METAL   368   368        Zinc; catalytic (By similarity). 
METAL   388   388        Zinc; catalytic (By similarity). 
METAL   399   399        Calcium 1 (Potential). 
METAL   399   399        Calcium 2 (Potential). 
METAL   402   402        Calcium 1 (Potential). 
METAL   402   402        Calcium 2 (Potential). 
METAL   404   404        Calcium 1; via carbonyl oxygen (Potential). 
METAL   407   407        Calcium 1 (Potential). 
METAL   407   407        Calcium 2 (Potential). 
CONFLICT   10    10        A -> R (in Ref. 1 and 2). 
CONFLICT   44    45        NA -> KP (in Ref. 1 and 2). 
CONFLICT   77    78        RL -> SV (in Ref. 1 and 2). 
Sequence information
Length: 521 AA [This is the length of the unprocessed precursor] Molecular weight: 56522 Da [This is the MW of the unprocessed precursor] CRC64: 439E7B8F19D1E8F4 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGLGKKLSVA VAASFMSLSI SLPGVQAAEG HQLKENQTNF LSKNAIAQSE LSAPNDKAVK 

        70         80         90        100        110        120 
QFLKKNSNIF KGDPSKRLKL VESTTDALGY KHFRYAPVVN GVPIKDSQVI VHVDKSDNVY 

       130        140        150        160        170        180 
AVNGELHNQS AAKTDNSQKV SSEKALALAF KAIGKSPDAV SNGAAKNSNK AELKAIETKD 

       190        200        210        220        230        240 
GSYRLAYDVT IRYVEPEPAN WEVLVDAETG SILKQQNKVE HAAATGSGTT LKGATVPLNI 

       250        260        270        280        290        300 
SYEGGKYVLR DLSKPTGTQI ITYDLQNRQS RLPGTLVSST TKTFTSSSQR AAVDAHYNLG 

       310        320        330        340        350        360 
KVYDYFYSNF KRNSYDNKGS KIVSSVHYGT QYNNAAWTGD QMIYGDGDGS FFSPLSGSLD 

       370        380        390        400        410        420 
VTAHEMTHGV TQETANLIYE NQPGALNESF SDVFGYFNDT EDWDIGEDIT VSQPALRSLS 

       430        440        450        460        470        480 
NPTKYNQPDN YANYRNLPNT DEGDYGGVHT NSGIPNKAAY NTITKLGVSK SQQIYYRALT 

       490        500        510        520 
TYLTPSSTFK DAKAALIQSA RDLYGSTDAA KVEAAWNAVG L 

P68736 in FASTA format

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