NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
TISSUE=Testis;
PubMed=7826319 [NCBI, ExPASy, EBI, Israel, Japan]
Wing S.S., Jain P.;
"Molecular cloning, expression and characterization of a ubiquitin conjugation enzyme (E2(17)kB) highly expressed in rat testis.";
Biochem. J. 305:125-132(1995).

Comments

FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53 (By similarity).
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
PATHWAY: Protein modification; protein ubiquitination .
SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex (By similarity).
TISSUE SPECIFICITY: Highly expressed in testis.
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U13176; AAA85101.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

S53359; S53359.

NP_001032369.1; -.

3D structure databases

HSSP

P15731; 1QCQ. [HSSP ENTRY / PDB]

SMR

P62839; 1-147.

ModBase

P62839.

Organism-specific databases

RGD

1591897; Ube2d2.

Gene expression databases

ArrayExpress

P62839; -.

GermOnline

ENSRNOG00000013741; Rattus norvegicus.

Family and domain databases

InterPro

IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.

PANTHER

PTHR11621; UBQ-conjugat_E2; 1.

Pfam

PF00179; UQ_con; 1.
Pfam graphical view of domain structure.

ProDom

PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00212; UBCc; 1.
SMART graphical view of domain structure.

PROSITE

PS00183; UBIQUITIN_CONJUGAT_1; 1.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P62839.

Genome annotation databases

Ensembl

ENSRNOG00000013741; Rattus norvegicus. [Contig view]

GeneID

641452; -.

KEGG

rno:641452; -.

Phylogenomic databases

HOVERGEN

P62839; -.

Other

ProtoNet

P62839.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Ligase; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 147`	`147`	`Ubiquitin-conjugating enzyme E2 D2.`	`PRO_0000082464`
`ACT_SITE`	`85 85`		`Glycyl thioester intermediate (By similarity).`

Sequence information

Length: 147 AA [This is the length of the unprocessed precursor]

Molecular weight: 16735 Da [This is the MW of the unprocessed precursor]

CRC64: C942BE7853CBC355 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY 

        70         80         90        100        110        120 
PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV 

       130        140 
PEIARIYKTD REKYNRIARE WTQKYAM

P62839 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools