ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with beta.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P62191


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name PRS4_HUMAN
Primary accession number P62191
Secondary accession numbers P49014 Q03527 Q6IAW0 Q96AZ3
Integrated into Swiss-Prot on June 21, 2004
Sequence was last modified on June 21, 2004 (Sequence version 1)
Annotations were last modified on    April 8, 2008 (Entry version 50)
Name and origin of the protein
Protein name 26S protease regulatory subunit 4
Synonyms P26s4
Proteasome 26S subunit ATPase 1
Gene name
Name: PSMC1
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
PubMed=1429620 [NCBI, ExPASy, EBI, Israel, Japan]
Dubiel W., Ferrell K., Pratt G., Rechsteiner M.C.;
"Subunit 4 of the 26 S protease is a member of a novel eukaryotic ATPase family.";
J. Biol. Chem. 267:22699-22702(1992).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).";
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Bone marrow, Lung, and Prostate;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
INTERACTION WITH SCA7.
DOI=10.1093/hmg/10.24.2821; PubMed=11734547 [NCBI, ExPASy, EBI, Israel, Japan]
Matilla A., Gorbea C., Einum D.D., Townsend J., Michalik A., van Broeckhoven C., Jensen C.C., Murphy K.J., Ptacek L.J., Fu Y.H.;
"Association of ataxin-7 with the proteasome subunit S4 of the 19S regulatory complex.";
Hum. Mol. Genet. 10:2821-2831(2001).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-439, AND MASS SPECTROMETRY.
TISSUE=T-cell;
DOI=10.1021/ac035352d; PubMed=15144186 [NCBI, ExPASy, EBI, Israel, Japan]
Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.;
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.";
Anal. Chem. 76:2763-2772(2004).
[6]
INTERACTION WITH NGLY1.
DOI=10.1073/pnas.0405663101; PubMed=15358861 [NCBI, ExPASy, EBI, Israel, Japan]
Katiyar S., Li G., Lennarz W.J.;
"A complex between peptide:N-glycanase and two proteasome-linked proteins suggests a mechanism for the degradation of misfolded glycoproteins.";
Proc. Natl. Acad. Sci. U.S.A. 101:13774-13779(2004).
[7]
INTERACTION WITH PAAF1.
DOI=10.1128/MCB.25.9.3842-3853.2005; PubMed=15831487 [NCBI, ExPASy, EBI, Israel, Japan]
Park Y., Hwang Y.-P., Lee J.-S., Seo S.-H., Yoon S.K., Yoon J.-B.;
"Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases.";
Mol. Cell. Biol. 25:3842-3853(2005).
[8]
MYRISTOYLATION [LARGE SCALE ANALYSIS] AT GLY-2, AND MASS SPECTROMETRY.
DOI=10.1021/bi061994u; PubMed=17323924 [NCBI, ExPASy, EBI, Israel, Japan]
Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L.;
"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.";
Biochemistry 46:3553-3565(2007).
[9]
UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-237, AND MASS SPECTROMETRY.
DOI=10.1002/pmic.200600410; PubMed=17370265 [NCBI, ExPASy, EBI, Israel, Japan]
Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.;
"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.";
Proteomics 7:868-874(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L02426; AAA35484.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CR457044; CAG33325.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC000512; AAH00512.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC016368; AAH16368.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC073818; AAH73818.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A44468; A44468.
RefSeq NP_002793.2; -.
UniGene Hs.356654
3D structure databases
HSSP P43773; 1OFI. [HSSP ENTRY / PDB]
ModBase P62191.
Protein-protein interaction databases
IntAct P62191; -.
PTM databases
PhosphoSite P62191; -.
Enzyme and pathway databases
Reactome REACT_11045; Signaling by Wnt.
REACT_152; Cell Cycle, Mitotic.
REACT_1538; Cell Cycle Checkpoints.
REACT_383; DNA Replication.
REACT_6185; HIV Infection.
REACT_6850; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_9035; APC/C:Cdh1-mediated degradation of Skp2.
Organism-specific databases
H-InvDB HIX0011881; -.
HIX0030744; -.
HGNC HGNC:9547; PSMC1.
GeneLynx PSMC1; Homo sapiens.
GenAtlas PSMC1.
HPA HPA000872; -.
MIM 602706; gene. [NCBI / EBI]
PharmGKB PA33892; -.
GeneCards P62191.
Gene expression databases
ArrayExpress P62191; -.
CleanEx HS_PSMC1; -.
GermOnline ENSG00000100764; Homo sapiens.
Ontologies
GO
GO:0000502; Cellular component: proteasome complex (non-traceable author statement from ProtInc).
GO:0016887; Molecular function: ATPase activity (traceable author statement from ProtInc).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR005937; 26S_proteasome_P45.
IPR003593; AAA+_ATPase_core.
IPR003959; AAA_ATPase_core.
IPR003960; AAA_ATPase_CS.
Graphical view of domain structure.
Pfam PF00004; AAA; 1.
Pfam graphical view of domain structure.
SMART SM00382; AAA; 1.
SMART graphical view of domain structure.
TIGRFAMs TIGR01242; 26Sp45; 1.
PROSITE PS00674; AAA; 1.
BLOCKS P62191.
Proteomic databases
PeptideAtlas P62191; -.
Genome annotation databases
Ensembl ENSG00000100764; Homo sapiens. [Contig view]
GeneID 5700; -.
KEGG hsa:5700; -.
Other
LinkHub P62191; -.
SOURCE PSMC1; Homo sapiens.
ProtoNet P62191.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Cytoplasm; Direct protein sequencing; Lipoprotein; Myristate; Nucleotide-binding; Nucleus; Phosphoprotein; Proteasome; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   440  439     26S protease regulatory subunit 4. PRO_0000084677
NP_BIND   226   233  8     ATP (Potential). 
MOD_RES   439   439        Phosphotyrosine. 
LIPID   2     2        N-myristoyl glycine. 
CROSSLNK   237   237        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin). 
CONFLICT   19    19        K -> E (in Ref. 1; AAA35484). 
CONFLICT   120   120        H -> R (in Ref. 3; AAH16368). 
Sequence information
Length: 440 AA [This is the length of the unprocessed precursor] Molecular weight: 49185 Da [This is the MW of the unprocessed precursor] CRC64: ACA80782F4F96F49 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGQSQSGGHG PGGGKKDDKD KKKKYEPPVP TRVGKKKKKT KGPDAASKLP LVTPHTQCRL 

        70         80         90        100        110        120 
KLLKLERIKD YLLMEEEFIR NQEQMKPLEE KQEEERSKVD DLRGTPMSVG TLEEIIDDNH 

       130        140        150        160        170        180 
AIVSTSVGSE HYVSILSFVD KDLLEPGCSV LLNHKVHAVI GVLMDDTDPL VTVMKVEKAP 

       190        200        210        220        230        240 
QETYADIGGL DNQIQEIKES VELPLTHPEY YEEMGIKPPK GVILYGPPGT GKTLLAKAVA 

       250        260        270        280        290        300 
NQTSATFLRV VGSELIQKYL GDGPKLVREL FRVAEEHAPS IVFIDEIDAI GTKRYDSNSG 

       310        320        330        340        350        360 
GEREIQRTML ELLNQLDGFD SRGDVKVIMA TNRIETLDPA LIRPGRIDRK IEFPLPDEKT 

       370        380        390        400        410        420 
KKRIFQIHTS RMTLADDVTL DDLIMAKDDL SGADIKAICT EAGLMALRER RMKVTNEDFK 

       430        440 
KSKENVLYKK QEGTPEGLYL 

P62191 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with beta.uniprot.org. Please send us your feedback!