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UniProtKB/Swiss-Prot entry P57087


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name JAM2_HUMAN
Primary accession number P57087
Secondary accession numbers B2R6T9 Q6UXG6 Q6YNC1
Integrated into Swiss-Prot on December 1, 2000
Sequence was last modified on December 1, 2000 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 85)
Name and origin of the protein
Protein name Junctional adhesion molecule B [Precursor]
Synonyms JAM-B
Junctional adhesion molecule 2
Vascular endothelial junction-associated molecule
VE-JAM
CD322 antigen
Gene name
Name: JAM2
Synonyms: C21orf43, VEJAM
ORFNames: UNQ219/PRO245
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-33, AND TISSUE SPECIFICITY.
TISSUE=Vascular endothelial cell;
DOI=10.1074/jbc.M003189200; PubMed=10779521 [NCBI, ExPASy, EBI, Israel, Japan]
Palmeri D., van Zante A., Huang C.-C., Hemmerich S., Rosen S.D.;
"Vascular endothelial junction-associated molecule, a novel member of the immunoglobulin superfamily, is localized to intercellular boundaries of endothelial cells.";
J. Biol. Chem. 275:19139-19145(2000).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Placenta;
DOI=10.1074/jbc.M002718200; PubMed=10945976 [NCBI, ExPASy, EBI, Israel, Japan]
Cunningham S.A., Arrate M.P., Rodriguez J.M., Bjercke R.J., Vanderslice P., Morris A.P., Brock T.A.;
"A novel protein with homology to the junctional adhesion molecule: Characterization of leukocyte interactions.";
J. Biol. Chem. 275:34750-34756(2000).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1006/geno.2002.6782; PubMed=12036298 [NCBI, ExPASy, EBI, Israel, Japan]
Gardiner K., Slavov D., Bechtel L., Davisson M.;
"Annotation of human chromosome 21 for relevance to Down syndrome: gene structure and expression analysis.";
Genomics 79:833-843(2002).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
DOI=10.1101/gr.1293003; PubMed=12975309 [NCBI, ExPASy, EBI, Israel, Japan]
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.";
Genome Res. 13:2265-2270(2003).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
DOI=10.1038/ng1285; PubMed=14702039 [NCBI, ExPASy, EBI, Israel, Japan]
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
"Complete sequencing and characterization of 21,243 full-length human cDNAs.";
Nat. Genet. 36:40-45(2004).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Lung;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[7]
PROTEIN SEQUENCE OF 29-43.
DOI=10.1110/ps.04682504; PubMed=15340161 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang Z., Henzel W.J.;
"Signal peptide prediction based on analysis of experimentally verified cleavage sites.";
Protein Sci. 13:2819-2824(2004).
[8]
INTERACTION WITH JAM3.
DOI=10.1074/jbc.M105972200; PubMed=11590146 [NCBI, ExPASy, EBI, Israel, Japan]
Arrate M.P., Rodriguez J.M., Tran T.M., Brock T.A., Cunningham S.A.;
"Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor.";
J. Biol. Chem. 276:45826-45832(2001).
[9]
REVIEW, AND NOMENCLATURE.
DOI=10.1016/S1471-4906(03)00117-0; PubMed=12810109 [NCBI, ExPASy, EBI, Israel, Japan]
Muller W.A.;
"Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response.";
Trends Immunol. 24:327-334(2003).
[10]
GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-98, AND MASS SPECTROMETRY.
TISSUE=Plasma;
DOI=10.1021/pr0502065; PubMed=16335952 [NCBI, ExPASy, EBI, Israel, Japan]
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.;
"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.";
J. Proteome Res. 4:2070-2080(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF255910; AAF81223.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY016009; AAG49022.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY077698; AAL82538.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY358361; AAQ88727.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK312708; BAG35586.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC017779; AAH17779.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00299083; -.
RefSeq NP_067042.1; -.
UniGene Hs.517227
3D structure databases
HSSP O88792; 1F97. [HSSP ENTRY / PDB]
ModBase P57087.
Enzyme and pathway databases
Pathway_Interaction_DB amb2_neutrophils_pathway; amb2 Integrin signaling.
Reactome REACT_13552; Integrin cell surface interactions.
REACT_604; Hemostasis.
REACT_6900; Signaling in Immune system.
Organism-specific databases
GeneCards GC21P025933; -.
H-InvDB HIX0016038; -.
HGNC HGNC:14686; JAM2.
GenAtlas JAM2.
MIM 606870; gene. [NCBI / EBI]
PharmGKB PA29992; -.
Gene expression databases
ArrayExpress P57087; -.
Bgee P57087; -.
CleanEx HS_JAM2; -.
GermOnline ENSG00000154721; Homo sapiens.
Ontologies
GO
GO:0005887; Cellular component: integral to plasma membrane (non-traceable author statement from UniProtKB).
GO:0005923; Cellular component: tight junction (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016337; Biological process: cell-cell adhesion (non-traceable author statement from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR013151; Ig.
IPR007110; Ig-like.
IPR013783; Ig-like_fold.
IPR003599; Ig_sub.
IPR003598; Ig_sub2.
IPR013106; Ig_V-set.
Graphical view of domain structure.
Gene3D G3DSA:2.60.40.10; Ig-like_fold; 2.
Pfam PF00047; ig; 1.
PF07686; V-set; 1.
Pfam graphical view of domain structure.
SMART SM00409; IG; 1.
SM00408; IGc2; 1.
SMART graphical view of domain structure.
PROSITE PS50835; IG_LIKE; 2.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE P57087; -.
Genome annotation databases
Ensembl ENSG00000154721; Homo sapiens. [Contig view]
GeneID 58494; -.
KEGG hsa:58494; -.
Phylogenomic databases
HOGENOM P57087; -.
HOVERGEN P57087; -.
Other
NextBio 64980; -.
SOURCE JAM2; Homo sapiens.
ProtoNet P57087.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell junction; Cell membrane; Direct protein sequencing; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Polymorphism; Signal; Tight junction; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    28  28      
CHAIN   29   298  270     Junctional adhesion molecule B. PRO_0000015069
TOPO_DOM   29   238  210     Extracellular (Potential). 
TRANSMEM   239   259  21     Potential. 
TOPO_DOM   260   298  39     Cytoplasmic (Potential). 
DOMAIN   32   127  96     Ig-like V-type. 
DOMAIN   134   238  105     Ig-like C2-type. 
CARBOHYD   98    98        N-linked (GlcNAc...). 
CARBOHYD   187   187        N-linked (GlcNAc...) (Potential). 
CARBOHYD   236   236        N-linked (GlcNAc...) (Potential). 
DISULFID   50   109        Potential. 
DISULFID   155   214        Potential. 
VARIANT   286   286  1     S -> R (in dbSNP:rs9976382 [NCBI]). VAR_049973 
CONFLICT   270   270        E -> G (in Ref. 3; AAL82538). 
CONFLICT   289   298        DFKHTKSFII -> VQWLTPVIPALWKAAAGGSRGQEF (in Ref. 4; AAQ88727). 
Sequence information
Length: 298 AA [This is the length of the unprocessed precursor] Molecular weight: 33207 Da [This is the MW of the unprocessed precursor] CRC64: CA78E518E22DCAEE [This is a checksum on the sequence]
        10         20         30         40         50         60 
MARRSRHRLL LLLLRYLVVA LGYHKAYGFS APKDQQVVTA VEYQEAILAC KTPKKTVSSR 

        70         80         90        100        110        120 
LEWKKLGRSV SFVYYQQTLQ GDFKNRAEMI DFNIRIKNVT RSDAGKYRCE VSAPSEQGQN 

       130        140        150        160        170        180 
LEEDTVTLEV LVAPAVPSCE VPSSALSGTV VELRCQDKEG NPAPEYTWFK DGIRLLENPR 

       190        200        210        220        230        240 
LGSQSTNSSY TMNTKTGTLQ FNTVSKLDTG EYSCEARNSV GYRRCPGKRM QVDDLNISGI 

       250        260        270        280        290 
IAAVVVVALV ISVCGLGVCY AQRKGYFSKE TSFQKSNSSS KATTMSENDF KHTKSFII 

P57087 in FASTA format

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