ID   CXA4A_CONPU             Reviewed;          25 AA.
AC   P55963;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   22-JUL-2008, entry version 48.
DE   RecName: Full=Alpha-A conotoxin PIVA;
OS   Conus purpurascens (Purple cone).
OC   Eukaryota; Metazoa; Mollusca; Gastropoda; Orthogastropoda;
OC   Apogastropoda; Caenogastropoda; Sorbeoconcha; Hypsogastropoda;
OC   Neogastropoda; Conoidea; Conidae; Conus.
OX   NCBI_TaxID=41690;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Venom;
RX   MEDLINE=95403432; PubMed=7673220; DOI=10.1074/jbc.270.38.22361;
RA   Hopkins C., Grilley M., Miller C., Shon K.-J., Cruz L.J., Gray W.R.,
RA   Dykert J., Rivier J.E., Yoshikami D., Olivera B.M.;
RT   "A new family of Conus peptides targeted to the nicotinic
RT   acetylcholine receptor.";
RL   J. Biol. Chem. 270:22361-22367(1995).
RN   [2]
RP   SYNTHESIS, AND FUNCTION.
RX   PubMed=16430227; DOI=10.1021/bi052016d;
RA   Teichert R.W., Lopez-Vera E., Gulyas J., Watkins M., Rivier J.,
RA   Olivera B.M.;
RT   "Definition and characterization of the short alphaA-conotoxins: a
RT   single residue determines dissociation kinetics from the fetal muscle
RT   nicotinic acetylcholine receptor.";
RL   Biochemistry 45:1304-1312(2006).
RN   [3]
RP   STRUCTURE BY NMR, AND DISULFIDE BONDS.
RX   MEDLINE=97200721; PubMed=9048550; DOI=10.1021/bi962301k;
RA   Han K.-H., Hwang K.-J., Kim S.-M., Kim S.-K., Gray W.R., Olivera B.M.,
RA   Rivier J.E., Shon K.-J.;
RT   "NMR structure determination of a novel conotoxin, [Pro 7,13] alpha A-
RT   conotoxin PIVA.";
RL   Biochemistry 36:1669-1677(1997).
CC   -!- FUNCTION: Alpha-A-long conotoxins act on postsynaptic membranes,
CC       they bind with high affinity to both fetal (alpha-1/beta-
CC       1/epsilon/delta subunits) and adult (alpha-1/beta-1/gamma/delta
CC       subunits) mammalian muscle nicotinic acetylcholine receptors
CC       (nAChR) and thus inhibit them. This toxin has higher affinity for
CC       the adult subtype (IC(50)=2.3 nM) of the receptor than for the
CC       fetal subtype (IC(50)=22 nM).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- SIMILARITY: Belongs to the conotoxin A superfamily. Alpha-A-type
CC       family. Alpha-A-long subfamily.
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DR   PIR; A58647; A58647.
DR   PDB; 1P1P; NMR; -; A=1-25.
DR   PDBsum; 1P1P; -.
DR   InterPro; IPR012498; Toxin_14.
DR   Pfam; PF07829; Toxin_14; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylcholine receptor inhibitor; Amidation;
KW   Direct protein sequencing; Hydroxylation; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Toxin.
FT   PEPTIDE       1     25       Alpha-A conotoxin PIVA.
FT                                /FTId=PRO_0000044466.
FT   MOD_RES       7      7       4-hydroxyproline; partial.
FT   MOD_RES      13     13       4-hydroxyproline; partial.
FT   MOD_RES      20     20       4-hydroxyproline.
FT   MOD_RES      25     25       Glutamine amide.
FT   DISULFID      2     16
FT   DISULFID      3     11
FT   DISULFID     14     23
FT   TURN         13     15
FT   HELIX        21     23
SQ   SEQUENCE   25 AA;  2608 MW;  9E2147898D697640 CRC64;
     GCCGSYPNAA CHPCSCKDRP SYCGQ
//