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UniProtKB/Swiss-Prot entry P54885


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PROA_YEAST
Primary accession number P54885
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on October 1, 1996 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 76)
Name and origin of the protein
Protein name Gamma-glutamyl phosphate reductase
Synonyms GPR
EC 1.2.1.41
Glutamate-5-semialdehyde dehydrogenase
GSA dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene name
Name: PRO2
OrderedLocusNames: YOR323C
ORFNames: O6155
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Lee J.M., Greenleaf A.L.;
"Mutations in Saccharomyces cerevisiae CTD kinase gene CTK1 are synthetic lethals with mutant versions of SSD1 and PRO2.";
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
DOI=10.1002/(SICI)1097-0061(199609)12:10B<1021::AID-YEA981>3.3.CO;2-Z; PubMed=8896266 [NCBI, ExPASy, EBI, Israel, Japan]
Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A., Schweizer M.;
"Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII.";
Yeast 12:1021-1031(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169874 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
Nature 387:98-102(1997).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U43565; AAA86261.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X90565; CAA62179.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z75231; CAA99643.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY692984; AAT93003.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S58334; S58334.
RefSeq NP_014968.1; -.
3D structure databases
PDB
1VLU; X-ray; 2.29 A; A/B=1-456.[ExPASy / RCSB / EBI]
PDBsum 1VLU; -.
ModBase P54885.
Protein-protein interaction databases
DIP DIP:6586N; -.
IntAct P54885; -.
Organism-specific databases
CYGD YOR323c; -.
SGD S000005850; PRO2.
Yeast-GFP YOR323C.
Gene expression databases
GermOnline YOR323C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from SGD).
GO:0005634; Cellular component: nucleus (inferred from direct assay from SGD).
GO:0004350; Molecular function: glutamate-5-semialdehyde dehydrogenase activity (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006561; Biological process: proline biosynthetic process (inferred from direct assay from SGD).
QuickGo view.
Family and domain databases
InterPro IPR016163; Ald_DHase_C.
IPR016162; Ald_DHase_N.
IPR000965; Gglut_pp_reduct.
IPR012134; Glu-5-SA_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.309.10; Aldehyde_dehydrogenase_C; 1.
G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
PIRSF PIRSF000151; GPR; 1.
TIGRFAMs TIGR00407; proA; 1.
PROSITE PS01223; PROA; 1.
BLOCKS P54885.
Proteomic databases
PeptideAtlas P54885; -.
Genome annotation databases
Ensembl YOR323C; Saccharomyces cerevisiae. [Contig view]
GeneID 854501; -.
GenomeReviews Y13140_GR; YOR323C.
KEGG sce:YOR323C; -.
NMPDR fig|4932.3.peg.6083; -.
Phylogenomic databases
HOGENOM P54885; -.
Other
LinkHub P54885; -.
ProtoNet P54885.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Amino-acid biosynthesis; Complete proteome; NADP; Oxidoreductase; Proline biosynthesis.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   456  456     Gamma-glutamyl phosphate reductase. PRO_0000189824
HELIX   3    18  16      
HELIX   23    39  17      
HELIX   41    57  17      
HELIX   62    68  7      
HELIX   75    88  14      
STRAND   92    94  3      
STRAND   96   103  8      
STRAND   106   114  9      
STRAND   117   124  8      
HELIX   127   139  13      
STRAND   142   146  5      
HELIX   149   151  3      
HELIX   152   173  22      
STRAND   179   182  4      
HELIX   190   194  5      
TURN   196   198  3      
STRAND   201   206  6      
HELIX   208   216  9      
STRAND   230   234  5      
HELIX   240   249  10      
STRAND   262   266  5      
HELIX   273   284  12      
STRAND   288   290  3      
HELIX   292   305  14      
HELIX   310   313  4      
STRAND   335   338  4      
HELIX   342   349  8      
STRAND   357   361  5      
HELIX   365   374  10      
STRAND   378   384  7      
HELIX   386   388  3      
HELIX   416   418  3      
STRAND   419   430  12      
Sequence information
Length: 456 AA [This is the length of the unprocessed precursor] Molecular weight: 49740 Da [This is the MW of the unprocessed precursor] CRC64: 7EDEC40C9B3DB07D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSSSQQIAKN ARKAGNILKT ISNEGRSDIL YKIHDALKAN AHAIEEANKI DLAVAKETGL 

        70         80         90        100        110        120 
ADSLLKRLDL FKGDKFEVML QGIKDVAELE DPVGKVKMAR ELDDGLTLYQ VTAPVGVLLV 

       130        140        150        160        170        180 
IFESRPEVIA NITALSIKSG NAAILKGGKE SVNTFREMAK IVNDTIAQFQ SETGVPVGSV 

       190        200        210        220        230        240 
QLIETRQDVS DLLDQDEYID LVVPRGSNAL VRKIKDTTKI PVLGHADGIC SIYLDEDADL 

       250        260        270        280        290        300 
IKAKRISLDA KTNYPAGCNA METLLINPKF SKWWEVLENL TLEGGVTIHA TKDLKTAYFD 

       310        320        330        340        350        360 
KLNELGKLTE AIQCKTVDAD EEQDFDKEFL SLDLAAKFVT STESAIQHIN THSSRHTDAI 

       370        380        390        400        410        420 
VTENKANAEK FMKGVDSSGV YWNASTRFAD GFRYGFGAEV GISTSKIHAR GPVGLDGLVS 

       430        440        450 
YQYQIRGDGQ VASDYLGAGG NKAFVHKDLD IKTVTL 

P54885 in FASTA format

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