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UniProtKB/Swiss-Prot entry P54227


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name STMN1_MOUSE
Primary accession number P54227
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    June 10, 2008 (Entry version 74)
Name and origin of the protein
Protein name Stathmin
Synonyms Phosphoprotein p19
pp19
Leukemia-associated phosphoprotein p18
Oncoprotein 18
Op18
pp17
Prosolin
Metablastin
Protein Pr22
Leukemia-associated gene protein
Gene name
Name: Stmn1
Synonyms: Lag, Lap18, Pr22
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1006/geno.1993.1477; PubMed=8288240 [NCBI, ExPASy, EBI, Israel, Japan]
Okazaki T., Yoshida B.N., Avraham K.B., Wang H., Wuenschell C.W., Jenkins N.A., Copeland N.G., Anderson D.J., Mori N.;
"Molecular diversity of the SCG10/stathmin gene family in the mouse.";
Genomics 18:360-373(1993).
[2]
ERRATUM.
Okazaki T., Yoshida B.N., Avraham K.B., Wang H., Wuenschell C.W., Jenkins N.A., Copeland N.G., Anderson D.J., Mori N.;
Genomics 21:298-298(1994).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=C57BL/6;
PubMed=8906359 [NCBI, ExPASy, EBI, Israel, Japan]
Hosoya H., Ishikawa K., Dohi N., Marunouchi T.;
"Transcriptional and post-transcriptional regulation of pr22 (Op18) with proliferation control.";
Cell Struct. Funct. 21:237-243(1996).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=129;
TISSUE=Mammary gland, and Retina;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
PROTEIN SEQUENCE OF 15-27, AND MASS SPECTROMETRY.
TISSUE=Brain, and Hippocampus;
Lubec G., Klug S., Yang J.W., Zigmond M.;
Submitted (JUL-2007) to UniProtKB.
[6]
PHOSPHORYLATION AT SER-16; SER-25; SER-38 AND SER-63.
PubMed=8376365 [NCBI, ExPASy, EBI, Israel, Japan]
Beretta L., Dobransky T., Sobel A.;
"Multiple phosphorylation of stathmin. Identification of four sites phosphorylated in intact cells and in vitro by cyclic AMP-dependent protein kinase and p34cdc2.";
J. Biol. Chem. 268:20076-20084(1993).
[7]
INTERACTION WITH KIST.
TISSUE=Embryo;
PubMed=7724523 [NCBI, ExPASy, EBI, Israel, Japan]
Maucuer A., Camonis J.H., Sobel A.;
"Stathmin interaction with a putative kinase and coiled-coil-forming protein domains.";
Proc. Natl. Acad. Sci. U.S.A. 92:3100-3104(1995).
[8]
FUNCTION IN CONTROL OF FEAR, AND TISSUE SPECIFICITY.
DOI=10.1016/j.cell.2005.08.038; PubMed=16286011 [NCBI, ExPASy, EBI, Israel, Japan]
Shumyatsky G.P., Malleret G., Shin R.-M., Takizawa S., Tully K., Tsvetkov E., Zakharenko S.S., Joseph J., Vronskaya S., Yin D., Schubart U.K., Kandel E.R., Bolshakov V.Y.;
"Stathmin, a gene enriched in the amygdala, controls both learned and innate fear.";
Cell 123:697-709(2005).
[9]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25; SER-38 AND SER-46, AND MASS SPECTROMETRY.
TISSUE=Brain cortex;
DOI=10.1074/mcp.M600046-MCP200; PubMed=17114649 [NCBI, ExPASy, EBI, Israel, Japan]
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.";
Mol. Cell. Proteomics 6:283-293(2007).
Comments
  • FUNCTION: Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis (By similarity). Involved in the control of the learned and innate fear.
  • SUBUNIT: Binds to two alpha/beta-tubulin heterodimers. Interacts with KIST.
  • INTERACTION:
    P46414:Cdkn1b; NbExp=2; IntAct=EBI-1006438, EBI-1005742;
  • SUBCELLULAR LOCATION: Cytoplasm.
  • TISSUE SPECIFICITY: Highly expressed in the lateral nucleus of the amygdala.
  • PTM: Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF (Probable). Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity).
  • MISCELLANEOUS: Mice lacking Stmn1 show deficits in spike-timing-dependent long-term potentiation, exhibit decreased memory in amygdala-dependent fear conditioning and fail to recognize danger in innately aversive environements.
  • SIMILARITY: Belongs to the stathmin family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L20256; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
L20257; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
L20258; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
X94915; CAA64401.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC010581; AAH10581.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC031831; AAH31831.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC054396; AAH54396.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B48917; B48917.
RefSeq NP_062615.1; -.
UniGene Mm.378957
3D structure databases
ModBase P54227.
Protein-protein interaction databases
IntAct P54227; -.
PTM databases
PhosphoSite P54227; -.
2D gel databases
REPRODUCTION-2DPAGE P54227; -.
Organism-specific databases
MGI MGI:96739; Stmn1.
GeneLynx Stmn1; Mus musculus.
Gene expression databases
ArrayExpress P54227; -.
CleanEx MM_STMN1; -.
GermOnline ENSMUSG00000028832; Mus musculus.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from MGI).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from UniProtKB).
GO:0007409; Biological process: axonogenesis (inferred from mutant phenotype from MGI).
GO:0031115; Biological process: negative regulation of microtubule polymerization (inferred from direct assay from MGI).
GO:0051272; Biological process: positive regulation of cell motility (inferred from genetic interaction from MGI).
QuickGo view.
Family and domain databases
InterPro IPR000956; Stathmin.
Graphical view of domain structure.
PANTHER PTHR10104; Stathmin; 1.
Pfam PF00836; Stathmin; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF002285; Stathmin; 1.
PRINTS PR00345; STATHMIN.
PROSITE PS00563; STATHMIN_1; 1.
PS01041; STATHMIN_2; 1.
BLOCKS P54227.
Genome annotation databases
Ensembl ENSMUSG00000028832; Mus musculus. [Contig view]
GeneID 16765; -.
KEGG mmu:16765; -.
Phylogenomic databases
HOGENOM P54227; -.
HOVERGEN P54227; -.
Other
SOURCE Stmn1; Mus musculus.
ProtoNet P54227.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Coiled coil; Cytoplasm; Developmental protein; Differentiation; Direct protein sequencing; Microtubule; Neurogenesis; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed (By similarity). 
CHAIN   2   149  148     Stathmin. PRO_0000182390
COILED   41   140  100     Potential. 
MOD_RES   2     2        N-acetylalanine (By similarity). 
MOD_RES   16    16        Phosphoserine; by PKA. 
MOD_RES   25    25        Phosphoserine; by CDC2 and MAPK. 
MOD_RES   28    28        Phosphoserine (By similarity). 
MOD_RES   38    38        Phosphoserine; by CDC2. 
MOD_RES   46    46        Phosphoserine. 
MOD_RES   63    63        Phosphoserine; by PKA. 
MOD_RES   146   146        Phosphothreonine (By similarity). 
Sequence information
Length: 149 AA [This is the length of the unprocessed precursor] Molecular weight: 17274 Da [This is the MW of the unprocessed precursor] CRC64: 616526E0A6667BDA [This is a checksum on the sequence]
        10         20         30         40         50         60 
MASSDIQVKE LEKRASGQAF ELILSPRSKE SVPDFPLSPP KKKDLSLEEI QKKLEAAEER 

        70         80         90        100        110        120 
RKSHEAEVLK QLAEKREHEK EVLQKAIEEN NNFSKMAEEK LTHKMEANKE NREAQMAAKL 

       130        140 
ERLREKDKHV EEVRKNKESK DPADETEAD 

P54227 in FASTA format

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