[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=BALB/c; TISSUE=Liver; PubMed=9224647 [NCBI, ExPASy, EBI, Israel, Japan] Mustonen M.; "Cloning of mouse 17beta-hydroxysteroid dehydrogenase type 2, and analysing expression of the mRNAs for types 1, 2, 3, 4 and 5 in mouse embryos and adult tissues."; Biochem. J. 325:199-205(1997).
[2]	NUCLEOTIDE SEQUENCE [MRNA] OF 1-358. STRAIN=BALB/c; Stoffel W., Weiss B.; Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH (By similarity).
CATALYTIC ACTIVITY: Estradiol-17-beta + NAD(P)⁺ = estrone + NAD(P)H.
CATALYTIC ACTIVITY: Testosterone + NAD⁺ = androst-4-ene-3,17-dione + NADH.
SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein (Potential).
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Y09517; CAA70706.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X95685; CAA64982.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_032316.2; -.

UniGene

Mm.276466

3D structure databases

HSSP

P14061; 1FDS. [HSSP ENTRY / PDB]

ModBase

P51658.

PTM databases

PhosphoSite

P51658; -.

Organism-specific databases

MGI

MGI:1096386; Hsd17b2.

Gene expression databases

ArrayExpress

P51658; -.

CleanEx

MM_HSD17B2; -.

GermOnline

ENSMUSG00000031844; Mus musculus.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

PROSITE

PS00061; ADH_SHORT; 1.

BLOCKS

P51658.

Genome annotation databases

Ensembl

ENSMUSG00000031844; Mus musculus. [Contig view]

GeneID

15486; -.

KEGG

mmu:15486; -.

Phylogenomic databases

HOGENOM

P51658; -.

HOVERGEN

P51658; -.

Other

SOURCE

Hsd17b2; Mus musculus.

ProtoNet

P51658.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Lipid synthesis; Membrane; NAD; Oxidoreductase; Signal-anchor; Steroid biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 381`	`381`	`Estradiol 17-beta-dehydrogenase 2.`	`PRO_0000054571`
`TRANSMEM`	`4 24`	`21`	`Signal-anchor for type II membrane protein (Potential).`
`NP_BIND`	`83 112`	`30`	`NAD (By similarity).`
`ACT_SITE`	`233 233`		`Proton acceptor (By similarity).`
`BINDING`	`220 220`		`Substrate (By similarity).`
`CONFLICT`	`36 37`		`QA -> RP (in Ref. 2; CAA64982).`

Sequence information

Length: 381 AA [This is the length of the unprocessed precursor]

Molecular weight: 41836 Da [This is the MW of the unprocessed precursor]

CRC64: 74A62797947E6086 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSPFASESAW LCLAAAAVLG GTLLCGCRSG RQLRSQAVCL AGLWGGACLL SLSLLCTLFL 

        70         80         90        100        110        120 
LSVACFLLLY MSSSDQDLLP VDQKAVLVTG ADSGFGHGLA KHLDKLGFTV FAGVLDKEGP 

       130        140        150        160        170        180 
GAEELRKHCS ERLSVLQMDV TKPEQIKDAH SKVTEKIQDK GLWAVVNNAG VFHLPIDGEL 

       190        200        210        220        230        240 
IPMSIYRKCM AVNFFGTVEV TKAFLPLLRK SKGRLVNVSS MGGTVPLQMT SAYAATKAAL 

       250        260        270        280        290        300 
TMFSTIIRQE LDKWGVKVVT IKPGGFKTNI TGSQDIWDKM EKEILDHFSK DIQENYGQDY 

       310        320        330        340        350        360 
VHTQKLIIPT LKERSNPDIT PVLRDIQHAI SARNPSSFYY PGRMAYLWVC LAAYCPTSLL 

       370        380 
DYVIKKGFYP QPTPRALRTV H

P51658 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools