[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION. DOI=10.1083/jcb.127.3.751; PubMed=7962057 [NCBI, ExPASy, EBI, Israel, Japan] Johnson D.R., Knoll L.J., Levin D.E., Gordon J.I.; "Saccharomyces cerevisiae contains four fatty acid activation (FAA) genes: an assessment of their role in regulating protein N-myristoylation and cellular lipid metabolism."; J. Cell Biol. 127:751-762(1994).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=ATCC 204511 / S288c / AB972; PubMed=9169872 [NCBI, ExPASy, EBI, Israel, Japan] Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Skelton J., Walsh S.V., Whitehead S., Barrell B.G.; "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."; Nature 387:90-93(1997).
[3]	LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan] Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.; "Global analysis of protein expression in yeast."; Nature 425:737-741(2003).

Comments

FUNCTION: Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Contributes, with FAA1, to the activation of imported myristate.
CATALYTIC ACTIVITY: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
COFACTOR: Magnesium (By similarity).
MISCELLANEOUS: Present with 31200 molecules/cell in log phase SD medium.
SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z48744; CAA88635.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Z48756; CAA88656.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S56060; S56060.

RefSeq

NP_013974.1; -.

3D structure databases

HSSP

P08659; 1LCI. [HSSP ENTRY / PDB]

ModBase

P47912.

Protein-protein interaction databases

DIP

DIP:4361N; -.

IntAct

P47912; 29.

Organism-specific databases

YMR246w; -.

S000004860; FAA4.

Gene expression databases

ArrayExpress

P47912; -.

GermOnline

YMR246W; Saccharomyces cerevisiae.

Ontologies

GO:0005811;	Cellular component: lipid particle (inferred from direct assay from SGD).
GO:0004467;	Molecular function: long-chain-fatty-acid-CoA ligase activity (inferred from electronic annotation from EC).
GO:0000287;	Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006631;	Biological process: fatty acid metabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0006869;	Biological process: lipid transport (inferred from genetic interaction from SGD).
GO:0006499;	Biological process: N-terminal protein myristoylation (inferred from genetic interaction from SGD).
QuickGo view.

Family and domain databases

InterPro

IPR000873; AMP-dep_Synth/Lig.
Graphical view of domain structure.

Pfam

PF00501; AMP-binding; 1.
Pfam graphical view of domain structure.

PRINTS

PR00154; AMPBINDING.

PROSITE

PS00455; AMP_BINDING; 1.

Proteomic databases

PeptideAtlas

P47912; -.

Proteomics databases

PRIDE

P47912; -.

Genome annotation databases

Ensembl

YMR246W; Saccharomyces cerevisiae. [Contig view]

855288; -.

Z71257_GR; YMR246W.

sce:YMR246W; -.

fig|4932.3.peg.5029; -.

Phylogenomic databases

HOGENOM

P47912; -.

Other

P47912; -.

978932; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Fatty acid metabolism; Ligase; Lipid metabolism; Magnesium.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 694`	`694`	`Long-chain-fatty-acid--CoA ligase 4.`	`PRO_0000193122`
`CONFLICT`	`552 552`		`N -> G (in Ref. 1; CAA88635).`
`CONFLICT`	`631 631`		`V -> G (in Ref. 1; CAA88635).`

Sequence information

Length: 694 AA [This is the length of the unprocessed precursor]

Molecular weight: 77267 Da [This is the MW of the unprocessed precursor]

CRC64: CF6524949C96D24D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTEQYSVAVG EAANEHETAP RRNIRVKDQP LIRPINSSAS TLYEFALECF TKGGKRDGMA 

        70         80         90        100        110        120 
WRDIIDIHET KKTIVKRVDG KDKPIEKTWL YYELTPYITM TYEEMICVMH DIGRGLIKIG 

       130        140        150        160        170        180 
VKPNGENKFH IFASTSHKWM KTFLGCMSQG IPVVTAYDTL GESGLIHSMV ETDSVAIFTD 

       190        200        210        220        230        240 
NQLLSKLAVP LKTAKNVKFV IHNEPIDPSD KRQNGKLYKA AKDAVDKIKE VRPDIKIYSF 

       250        260        270        280        290        300 
DEIIEIGKKA KDEVELHFPK PEDPACIMYT SGSTGTPKGV VLTHYNIVAG IGGVGHNVIG 

       310        320        330        340        350        360 
WIGPTDRIIA FLPLAHIFEL TFEFEAFYWN GILGYANVKT LTPTSTRNCQ GDLMEFKPTV 

       370        380        390        400        410        420 
MVGVAAVWET VRKGILAKIN ELPGWSQTLF WTVYALKERN IPCSGLLSGL IFKRIREATG 

       430        440        450        460        470        480 
GNLRFILNGG SAISIDAQKF LSNLLCPMLI GYGLTEGVAN ACVLEPEHFD YGIAGDLVGT 

       490        500        510        520        530        540 
ITAKLVDVED LGYFAKNNQG ELLFKGAPIC SEYYKNPEET AAAFTDDGWF RTGDIAEWTP 

       550        560        570        580        590        600 
KGQVKIIDRK KNLVKTLNGE YIALEKLESI YRSNPYVQNI CVYADENKVK PVGIVVPNLG 

       610        620        630        640        650        660 
HLSKLAIELG IMVPGEDVES YIHEKKLQDA VCKDMLSTAK SQGLNGIELL CGIVFFEEEW 

       670        680        690 
TPENGLVTSA QKLKRRDILA AVKPDVERVY KENT

P47912 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools