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[1]
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NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Testis;
DOI=10.1006/bbrc.1994.2738; PubMed=7999118 [NCBI, ExPASy, EBI, Israel, Japan]
Konishi H.,
Shinomura T.,
Kuroda S.I.,
Ono Y.,
Kikkawa U.;
"Molecular cloning of rat RAC protein kinase alpha and beta and their association with protein kinase C zeta.";
Biochem. Biophys. Res. Commun. 205:817-825(1994).
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[2]
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FUNCTION, AND MUTAGENESIS OF LYS-179; THR-308 AND SER-473.
PubMed=9632753 [NCBI, ExPASy, EBI, Israel, Japan]
Kitamura T.,
Ogawa W.,
Sakaue H.,
Hino Y.,
Kuroda S.,
Takata M.,
Matsumoto M.,
Maeda T.,
Konishi H.,
Kikkawa U.,
Kasuga M.;
"Requirement for activation of the serine-threonine kinase Akt (protein kinase B) in insulin stimulation of protein synthesis but not of glucose transport.";
Mol. Cell. Biol. 18:3708-3717(1998).
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[3]
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FUNCTION, PHOSPHORYLATION AT THR-308 AND SER-473, AND MUTAGENESIS OF LYS-179; THR-308 AND SER-473.
DOI=10.1074/jbc.274.29.20611; PubMed=10400692 [NCBI, ExPASy, EBI, Israel, Japan]
Takata M.,
Ogawa W.,
Kitamura T.,
Hino Y.,
Kuroda S.,
Kotani K.,
Klip A.,
Gingras A.-C.,
Sonenberg N.,
Kasuga M.;
"Requirement for Akt (protein kinase B) in insulin-induced activation of glycogen synthase and phosphorylation of 4E-BP1 (PHAS-1).";
J. Biol. Chem. 274:20611-20618(1999).
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- FUNCTION: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I (By similarity). Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
- ENZYME REGULATION: Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.
- SUBUNIT: The C-terminus interacts with the C-terminus of CCDC88A/GRDN and THEM4. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B (By similarity).
- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Cell membrane (By similarity). Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A (By similarity).
- TISSUE SPECIFICITY: Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.
- DOMAIN: Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
- DOMAIN: The AGC-kinase C-terminal mediates interaction with THEM4 (By similarity).
- PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation favors Thr-308 phosphorylation.
- SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
- SIMILARITY: Contains 1 AGC-kinase C-terminal domain.
- SIMILARITY: Contains 1 PH domain.
- SIMILARITY: Contains 1 protein kinase domain.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 480 AA [This is the length of the unprocessed precursor] |
Molecular weight: 55735 Da [This is the MW of the unprocessed precursor] |
CRC64: 5DCAAE7134366D04 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MNDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVEQRES PLNNFSVAQC
70 80 90 100 110 120
QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KRQEEETMDF
130 140 150 160 170 180
RSGSPSDNSG AEEMEVALAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI
190 200 210 220 230 240
LKKEVIVAKD EVAHTLTENR VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS
250 260 270 280 290 300
RERVFSEDRA RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI
310 320 330 340 350 360
KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHEKLFEL
370 380 390 400 410 420
ILMEEIRFPR TLGPEAKSLL SGLLKKDPTQ RLGGGSEDAK EIMQHRFFAN IVWQDVYEKK
430 440 450 460 470 480
LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEC VDSERRPHFP QFSYSASGTA
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P47196 in FASTA format |
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ExPASy Home page
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