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UniProtKB/Swiss-Prot entry P46573


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name APK1B_ARATH
Primary accession number P46573
Secondary accession number Q9SLH5
Integrated into Swiss-Prot on November 1, 1995
Sequence was last modified on December 1, 2000 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 72)
Name and origin of the protein
Protein name Protein kinase APK1B, chloroplastic [Precursor]
Synonym EC 2.7.11.1
Gene name
Name: APK1B
OrderedLocusNames: At2g28930
ORFNames: T9I4.1
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/45471; PubMed=10617197 [NCBI, ExPASy, EBI, Israel, Japan]
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
Nature 402:761-768(1999).
[2]
NUCLEOTIDE SEQUENCE [MRNA] OF 143-346.
STRAIN=cv. Columbia;
DOI=10.1007/BF00046450; PubMed=1450380 [NCBI, ExPASy, EBI, Israel, Japan]
Hirayama T., Oka A.;
"Novel protein kinase of Arabidopsis thaliana (APK1) that phosphorylates tyrosine, serine and threonine.";
Plant Mol. Biol. 20:653-662(1992).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AC005315; AAC33221.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D10152; BAA20968.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T02725; T02725.
RefSeq NP_001031439.1; -.
UniGene At.46050
3D structure databases
ModBase P46573.
Organism-specific databases
GeneFarm 1774; 129.
TAIR At2g28930; -.
Family and domain databases
InterPro IPR000719; Prot_kinase_core.
IPR017441; Protein_kinase_ATP_bd_CS.
IPR017442; Se/Thr_pkinase-rel.
IPR008271; Ser_thr_pkin_AS.
Graphical view of domain structure.
Pfam PF00069; Pkinase; 1.
Pfam graphical view of domain structure.
ProDom PD000001; Prot_kinase; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00107; PROTEIN_KINASE_ATP; 1.
PS50011; PROTEIN_KINASE_DOM; 1.
PS00108; PROTEIN_KINASE_ST; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P46573.
Genome annotation databases
GeneID 817442; -.
GenomeReviews CT485783_GR; AT2G28930.
KEGG ath:AT2G28930; -.
Other
ProtoNet P46573.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; ATP-binding; Chloroplast; Complete proteome; Kinase; Nucleotide-binding; Plastid; Serine/threonine-protein kinase; Transferase; Transit peptide; Tyrosine-protein kinase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
TRANSIT   1    59  59     Chloroplast (Potential). 
CHAIN   60   412  353     Protein kinase APK1B, chloroplastic. PRO_0000024303
DOMAIN   69   356  288     Protein kinase. 
NP_BIND   75    83  9     ATP (By similarity). 
ACT_SITE   204   204        Proton acceptor (By similarity). 
BINDING   107   107        ATP (By similarity). 
Sequence information
Length: 412 AA [This is the length of the unprocessed precursor] Molecular weight: 45746 Da [This is the MW of the unprocessed precursor] CRC64: EB1CA0B1A626A5DA [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGICLSAQIK AVSPGASPKY MSSEANDSLG SKSSSVSIRT NPRTEGEILQ SPNLKSFTFA 

        70         80         90        100        110        120 
ELKAATRNFR PDSVLGEGGF GSVFKGWIDE QTLTASKPGT GVVIAVKKLN QDGWQGHQEW 

       130        140        150        160        170        180 
LAEVNYLGQF SHPNLVKLIG YCLEDEHRLL VYEFMPRGSL ENHLFRRGSY FQPLSWTLRL 

       190        200        210        220        230        240 
KVALGAAKGL AFLHNAETSV IYRDFKTSNI LLDSEYNAKL SDFGLAKDGP TGDKSHVSTR 

       250        260        270        280        290        300 
IMGTYGYAAP EYLATGHLTT KSDVYSYGVV LLEVLSGRRA VDKNRPPGEQ KLVEWARPLL 

       310        320        330        340        350        360 
ANKRKLFRVI DNRLQDQYSM EEACKVATLA LRCLTFEIKL RPNMNEVVSH LEHIQTLNEA 

       370        380        390        400        410 
GGRNIDMVQR RMRRRSDSVA INQKPNAGFA RQTAVGVIAT AYPRPSDSPL FV 

P46573 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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