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UniProtKB/Swiss-Prot entry P46527


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CDN1B_HUMAN
Primary accession number P46527
Secondary accession numbers Q16307 Q9BUS6
Integrated into Swiss-Prot on November 1, 1995
Sequence was last modified on November 1, 1995 (Sequence version 1)
Annotations were last modified on    June 10, 2008 (Entry version 94)
Name and origin of the protein
Protein name Cyclin-dependent kinase inhibitor 1B
Synonyms Cyclin-dependent kinase inhibitor p27
p27Kip1
Gene name
Name: CDKN1B
Synonyms: KIP1
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 28-79 AND 104-152.
TISSUE=Kidney;
DOI=10.1016/0092-8674(94)90572-X; PubMed=8033212 [NCBI, ExPASy, EBI, Israel, Japan]
Polyak K., Lee M.-H., Erdjument-Bromage H., Koff A., Roberts J.M., Tempst P., Massague J.;
"Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals.";
Cell 78:59-66(1994).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=7882309 [NCBI, ExPASy, EBI, Israel, Japan]
Pietenpol J.A., Bohlander S.K., Sato Y., Papadopoulos N., Liu B., Friedman C., Trask B.J., Roberts J.M., Kinzler K.W., Rowley J.D.;
"Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias.";
Cancer Res. 55:1206-1210(1995).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS TRP-15 AND GLY-109.
Rieder M.J., Braun A.C., Montoya M.A., Chung M.-W., Nguyen C.P., Nguyen D.A., Livingston R.J., Poel C.L., Robertson P.D., Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A.;
"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).";
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLY-109.
TISSUE=Cervix;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
INTERACTION WITH UHMK1, PHOSPHORYLATION AT SER-10, AND MUTAGENESIS OF SER-10 AND THR-187.
DOI=10.1093/emboj/cdf343; PubMed=12093740 [NCBI, ExPASy, EBI, Israel, Japan]
Boehm M., Yoshimoto T., Crook M.F., Nallamshetty S., True A., Nabel G.J., Nabel E.G.;
"A growth factor-dependent nuclear kinase phosphorylates p27(Kip1) and regulates cell cycle progression.";
EMBO J. 21:3390-3401(2002).
[6]
INTERACTION WITH SPDYA.
DOI=10.1091/mbc.E02-12-0820; PubMed=12972555 [NCBI, ExPASy, EBI, Israel, Japan]
Porter L.A., Kong-Beltran M., Donoghue D.J.;
"Spy1 interacts with p27Kip1 to allow G1/S progression.";
Mol. Biol. Cell 14:3664-3674(2003).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1073/pnas.0404720101; PubMed=15302935 [NCBI, ExPASy, EBI, Israel, Japan]
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140, AND MASS SPECTROMETRY.
DOI=10.1126/science.1140321; PubMed=17525332 [NCBI, ExPASy, EBI, Israel, Japan]
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.";
Science 316:1160-1166(2007).
[9]
X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 23-106 OF COMPLEX WITH CDK2 AND CG2A.
DOI=10.1038/382325a0; PubMed=8684460 [NCBI, ExPASy, EBI, Israel, Japan]
Russo A.A., Jeffrey P.D., Patten A.K., Massague J., Pavletich N.P.;
"Crystal structure of the p27Kip1 cyclin-dependent-kinase inhibitor bound to the cyclin A-Cdk2 complex.";
Nature 382:325-331(1996).
[10]
INVOLVEMENT IN MEN4.
DOI=10.1073/pnas.0603877103; PubMed=17030811 [NCBI, ExPASy, EBI, Israel, Japan]
Pellegata N.S., Quintanilla-Martinez L., Siggelkow H., Samson E., Bink K., Hoefler H., Fend F., Graw J., Atkinson M.J.;
"Germ-line mutations in p27(Kip1) cause a multiple endocrine neoplasia syndrome in rats and humans.";
Proc. Natl. Acad. Sci. U.S.A. 103:15558-15563(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U10906; AAA20240.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S76988; AAD14244.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S76986; AAD14244.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF480891; AAL78041.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC001971; AAH01971.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_004055.1; -.
UniGene Hs.238990
3D structure databases
PDB
1H27; X-ray; 2.20 A; E=25-35.[ExPASy / RCSB / EBI]
1JSU; X-ray; 2.30 A; C=23-106.[ExPASy / RCSB / EBI]
2AST; X-ray; 2.30 A; D=181-190.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1H27; -.
1JSU; -.
2AST; -.
DisProt DP00018; -.
ModBase P46527.
Protein-protein interaction databases
IntAct P46527; -.
PTM databases
PhosphoSite P46527; -.
Polymorphism databases
NIEHS-SNPs CDKN1B.
2D gel databases
SWISS-2DPAGE P46527; -.
Organism-specific databases
H-InvDB HIX0010441; -.
HGNC HGNC:1785; CDKN1B.
GeneLynx CDKN1B; Homo sapiens.
GenAtlas CDKN1B.
HPA CAB003691; -.
MIM 600778; gene. [NCBI / EBI]
610755; phenotype. [NCBI / EBI]
PharmGKB PA105; -.
GeneCards P46527.
Gene expression databases
ArrayExpress P46527; -.
CleanEx HS_CDKN1B; -.
GermOnline ENSG00000111276; Homo sapiens.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from experiment from Reactome).
GO:0005634; Cellular component: nucleus (inferred from direct assay from HGNC).
GO:0004861; Molecular function: cyclin-dependent protein kinase inhibitor activity (traceable author statement from ProtInc).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0005072; Molecular function: transforming growth factor beta receptor, cytoplasmic mediator activity (traceable author statement from ProtInc).
GO:0048102; Biological process: autophagic cell death (inferred from direct assay from UniProtKB).
GO:0007050; Biological process: cell cycle arrest (inferred from mutant phenotype from HGNC).
GO:0000082; Biological process: G1/S transition of mitotic cell cycle (inferred from direct assay from UniProtKB).
GO:0006917; Biological process: induction of apoptosis (inferred from direct assay from UniProtKB).
GO:0030308; Biological process: negative regulation of cell growth (inferred from direct assay from UniProtKB).
GO:0008285; Biological process: negative regulation of cell proliferation (traceable author statement from ProtInc).
GO:0042326; Biological process: negative regulation of phosphorylation (inferred from direct assay from UniProtKB).
GO:0000079; Biological process: regulation of cyclin-dependent protein kinase activity (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR003175; CDI.
IPR015456; Cyclin_inhib_p27.
Graphical view of domain structure.
PANTHER PTHR10265; CDI; 1.
PTHR10265:SF4; p27_Kip1; 1.
Pfam PF02234; CDI; 1.
Pfam graphical view of domain structure.
BLOCKS P46527.
Genome annotation databases
Ensembl ENSG00000111276; Homo sapiens. [Contig view]
GeneID 1027; -.
KEGG hsa:1027; -.
Phylogenomic databases
HOGENOM P46527; -.
HOVERGEN P46527; -.
Other
LinkHub P46527; -.
SOURCE CDKN1B; Homo sapiens.
ProtoNet P46527.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Cell cycle; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Protein kinase inhibitor; Proto-oncogene.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   198  198     Cyclin-dependent kinase inhibitor 1B. PRO_0000190084
MOTIF   153   169  17     Nuclear localization signal (Potential). 
MOD_RES   10    10        Phosphoserine; by UHMK1. 
MOD_RES   140   140        Phosphoserine. 
VARIANT   15    15  1     R -> W (in dbSNP:rs2066828 [NCBI]). VAR_011871 
VARIANT   109   109  1     V -> G (in dbSNP:rs2066827 [NCBI]). VAR_011872 
MUTAGEN   10    10        S->A: Loss of phosphorylation by UHMK1; causes cell cycle arrest. 
MUTAGEN   187   187        T->A: No effect on phosphorylation by UHMK1. 
CONFLICT   22    22        E -> D (in Ref. 2; AAD14244). 
HELIX   38    49  12      
TURN   50    53  4      
HELIX   54    60  7      
TURN   64    67  4      
STRAND   71    74  4      
STRAND   77    80  4      
HELIX   86    89  4      
Sequence information
Length: 198 AA [This is the length of the unprocessed precursor] Molecular weight: 22073 Da [This is the MW of the unprocessed precursor] CRC64: 1118D58901CDF3FC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSNVRVSNGS PSLERMDARQ AEHPKPSACR NLFGPVDHEE LTRDLEKHCR DMEEASQRKW 

        70         80         90        100        110        120 
NFDFQNHKPL EGKYEWQEVE KGSLPEFYYR PPRPPKGACK VPAQESQDVS GSRPAAPLIG 

       130        140        150        160        170        180 
APANSEDTHL VDPKTDPSDS QTGLAEQCAG IRKRPATDDS STQNKRANRT EENVSDGSPN 

       190 
AGSVEQTPKK PGLRRRQT 

P46527 in FASTA format

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