[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1104/pp.107.4.1455; PubMed=7770532 [NCBI, ExPASy, EBI, Israel, Japan] Poeydomenge O., Boudet A.M., Grima-Pettenati J., Marolda M.; "Nucleotide sequence of a cDNA encoding mitochondrial malate dehydrogenase from Eucalyptus."; Plant Physiol. 107:1455-1456(1995).

Comments

CATALYTIC ACTIVITY: (S)-malate + NAD⁺ = oxaloacetate + NADH.
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion matrix.
SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X78800; CAA55383.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S44167; S44167.

3D structure databases

HSSP

P00346; 1MLD. [HSSP ENTRY / PDB]

ModBase

P46487.

Ontologies

GO:0005759;	Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001557; L-lactate/malate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR001252; Malate_DHase_AS.
IPR010097; Malate_DHase_NAD-dep_euk_g_bac.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR11540:SF1; MDH_euk_g_bac; 1.

Pfam

PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000102; Lac_mal_DH; 1.

TIGRFAMs

TIGR01772; MDH_euk_gproteo; 1.

PS00068; MDH; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Mitochondrion; NAD; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 27`	`27`	`Mitochondrion (Potential).`
`CHAIN`	`28 347`	`320`	`Malate dehydrogenase, mitochondrial.`	`PRO_0000018626`
`NP_BIND`	`41 47`	`7`	`NAD (By similarity).`
`NP_BIND`	`150 152`	`3`	`NAD (By similarity).`
`ACT_SITE`	`210 210`		`Proton acceptor (By similarity).`
`BINDING`	`67 67`		`NAD (By similarity).`
`BINDING`	`114 114`		`Substrate (By similarity).`
`BINDING`	`120 120`		`Substrate (By similarity).`
`BINDING`	`127 127`		`NAD (By similarity).`
`BINDING`	`152 152`		`Substrate (By similarity).`
`BINDING`	`186 186`		`Substrate (By similarity).`
`BINDING`	`261 261`		`NAD (By similarity).`

Sequence information

Length: 347 AA [This is the length of the unprocessed precursor]

Molecular weight: 36462 Da [This is the MW of the unprocessed precursor]

CRC64: 450F8254CCC84789 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRASMLRLIR SRSSSAAPRP HLLRRAYGSE SVPERKVAVL GAAGGIGQPL ALLMKLNPLV 

        70         80         90        100        110        120 
SQLALYDIAG TPGVAADVGH INTRSEVAGY VGEEQLGQAL EGSDVVIIPA GVPRKPGMTR 

       130        140        150        160        170        180 
DDLFNINAGI VKSLCTAIAK YCPNAVVNMI SNPVNSTVPI AAEIFKKAGT YNEKKLLGVT 

       190        200        210        220        230        240 
TLDVVRAKTF YAGKAKVPVE EVNVPVVGGH AGITILPLFS QAVPKANLAD EDIKALTKRT 

       250        260        270        280        290        300 
QDGGTEVVEA KAGKGSATLS MAYAGALFAD ACLKGLNGVP DVVECSFVQS SIITELPFFA 

       310        320        330        340 
SKVKLGKNGV EEVLELGPMS DYEKQGLEIL IPELKASIEK GIKFANQ

P46487 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools