[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Brain; Hong R., Moriyama Y., Mori H., Futai M., Yamamoto A., Tashiro Y., Fukui T., Tagaya M.; "Structure and localization of a brain N-ethylmaleimide-sensitive factor involved in vesicular transport."; Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [MRNA]. Bui T.D., Lu L., Hong W.; Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Brain; Zhang R., Liu Z., Wu M.; "The regulation of hNSF gene expression."; Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Brain; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[5]	IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY. Colinge J., Superti-Furga G., Bennett K.L.; Submitted (OCT-2008) to UniProtKB.

Comments

FUNCTION: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
CATALYTIC ACTIVITY: ATP + H₂O = ADP + phosphate.
COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
SUBUNIT: Homohexamer. Interacts with GABARAP and GABARAPL2 (By similarity).
INTERACTION:
Q05209:PTPN12; NbExp=1; IntAct=EBI-712251, EBI-2266035;
P23467:PTPRB; NbExp=1; IntAct=EBI-712251, EBI-1265766;
P08575:PTPRC; NbExp=1; IntAct=EBI-712251, EBI-1341;
P23470:PTPRG; NbExp=1; IntAct=EBI-712251, EBI-2258115;
Q12913:PTPRJ; NbExp=1; IntAct=EBI-712251, EBI-2264500;
Q15262:PTPRK; NbExp=1; IntAct=EBI-712251, EBI-474052;
Q16827:PTPRO; NbExp=1; IntAct=EBI-712251, EBI-723739;
O76081:RGS20; NbExp=1; IntAct=EBI-712251, EBI-1052678;
O00422:SAP18; NbExp=1; IntAct=EBI-712251, EBI-1044156;
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the AAA ATPase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U03985; AAA17411.1; ALT_INIT; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF135168; AAF70545.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF102846; AAF04745.2; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC030613; AAH30613.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI00006451; -.

G01234; G01234.

3D structure databases

HSSP

P18708; 1NSF. [HSSP ENTRY / PDB]

SMR

P46459; 1-201, 489-735.

ModBase

P46459.

Protein-protein interaction databases

IntAct

P46459; 18.

Protein family/group databases

TCDB

1.F.1.1.1; synaptosomal vesicle fusion pore (SVF-Pore) family.

PTM databases

PhosphoSite

P46459; -.

Enzyme and pathway databases

BRENDA

3.6.4.6; 247.

Organism-specific databases

GC17P042024; -.

HGNC:8016; NSF.

NSF.

CAB009324; -.
CAB013645; -.
HPA003154; -.

MIM

601633; gene. [NCBI / EBI]

PharmGKB

PA31793; -.

Gene expression databases

P46459; -.

P46459; -.

HS_NSF; -.

ENSG00000073969; Homo sapiens.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from direct assay from HPA).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004176;	Molecular function: ATP-dependent peptidase activity (inferred from electronic annotation from InterPro).
GO:0000287;	Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0004252;	Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0015031;	Biological process: protein transport (inferred from electronic annotation from UniProtKB-KW).
GO:0006508;	Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR003593; ATPase_AAA+_core.
IPR003959; ATPase_AAA_core.
IPR003960; ATPase_AAA_CS.
IPR003338; ATPase_AAA_VAT_N.
IPR004201; Cell_division_protein_CDC48_2.
IPR001984; Peptidase_S16_C.
Graphical view of domain structure.

Pfam

PF00004; AAA; 2.
PF02933; CDC48_2; 1.
PF02359; CDC48_N; 1.
Pfam graphical view of domain structure.

PRINTS

PR00830; ENDOLAPTASE.

SMART

SM00382; AAA; 2.
SMART graphical view of domain structure.

PROSITE

PS00674; AAA; 1.

Proteomic databases

PRIDE

P46459; -.

Genome annotation databases

Ensembl

ENSG00000073969; Homo sapiens. [Contig view]

KEGG

hsa:4905; -.

Phylogenomic databases

HOGENOM

P46459; -.

HOVERGEN

P46459; -.

Other

P46459; -.

NSF; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Hydrolase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Protein transport; Repeat; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 744`	`744`	`Vesicle-fusing ATPase.`	`PRO_0000084563`
`NP_BIND`	`260 267`	`8`	`ATP (Potential).`
`NP_BIND`	`543 550`	`8`	`ATP (Potential).`
`METAL`	`550 550`		`Magnesium (By similarity).`
`MOD_RES`	`259 259`		`Phosphotyrosine (By similarity).`
`VARIANT`	`476 476`	`1`	`T -> M (in dbSNP:rs155733 [NCBI]).`	`VAR_029580`
`CONFLICT`	`22 22`		`A -> S (in Ref. 1 and 2).`
`CONFLICT`	`107 107`		`I -> N (in Ref. 1, 2 and 3).`
`CONFLICT`	`130 130`		`F -> Y (in Ref. 1, 2 and 3).`
`CONFLICT`	`154 154`		`A -> S (in Ref. 1, 2 and 3).`
`CONFLICT`	`237 237`		`S -> F (in Ref. 1, 2 and 3).`
`CONFLICT`	`251 251`		`K -> I (in Ref. 1, 2 and 3).`
`CONFLICT`	`571 571`		`D -> E (in Ref. 3; AAF04745).`
`CONFLICT`	`686 686`		`L -> F (in Ref. 4; AAH30613).`

Sequence information

Length: 744 AA [This is the length of the unprocessed precursor]

Molecular weight: 82560 Da [This is the MW of the unprocessed precursor]

CRC64: BF5450200C74C8F7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAGRSMQAAR CPTDELSLTN CAVVNEKDFQ SGQHVIVRTS PNHRYTFTLK THPSVVPGSI 

        70         80         90        100        110        120 
AFSLPQRKWA GLSIGQEIEV SLYTFDKAKQ CIGTMTIEID FLQKKSIDSN PYDTDKMAAE 

       130        140        150        160        170        180 
FIQQFNNQAF SVGQQLVFSF NEKLFGLLVK DIEAMDPSIL KGEPATGKRQ KIEVGLVVGN 

       190        200        210        220        230        240 
SQVAFEKAEN SSLNLIGKAK TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF 

       250        260        270        280        290        300 
PPEIVEQMGC KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA 

       310        320        330        340        350        360 
NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV VNQLLSKIDG 

       370        380        390        400        410        420 
VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE KGRLQILHIH TARMRGHQLL 

       430        440        450        460        470        480 
SADVDIKELA VETKNFSGAE LEGLVRAAQS TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF 

       490        500        510        520        530        540 
LASLENDIKP AFGTNQEDYA SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL 

       550        560        570        580        590        600 
LEGPPHSGKT ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV 

       610        620        630        640        650        660 
VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD VLQEMEMLNA 

       670        680        690        700        710        720 
FSTTIHVPNI ATGEQLLEAL ELLGNLKDKE RTTIAQQVKG KKVWIGIKKL LMLIEMSLQM 

       730        740 
DPEYRVRKFL ALLREEGASP LDFD

P46459 in FASTA format

View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools