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UniProtKB/Swiss-Prot entry P44121


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DNLI_HAEIN
Primary accession number P44121
Secondary accession numbers P44122 P71371 P71372
Integrated into Swiss-Prot on November 1, 1995
Sequence was last modified on October 1, 1996 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 62)
Name and origin of the protein
Protein name DNA ligase
Synonyms EC 6.5.1.1
Polydeoxyribonucleotide synthase [ATP]
Gene name
Name: ligA
OrderedLocusNames: HI1182/HI1183
From
Haemophilus influenzae [TaxID: 727] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Haemophilus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
PubMed=8550458 [NCBI, ExPASy, EBI, Israel, Japan]
Preston A., Maskell D., Johnson A., Moxon E.R.;
"Altered lipopolysaccharide characteristic of the I69 phenotype in Haemophilus influenzae results from mutations in a novel gene, isn.";
J. Bacteriol. 178:396-402(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
PubMed=7542800 [NCBI, ExPASy, EBI, Israel, Japan]
Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.";
Science 269:496-512(1995).
[3]
CHARACTERIZATION.
DOI=10.1093/nar/25.7.1369; PubMed=9060431 [NCBI, ExPASy, EBI, Israel, Japan]
Cheng C., Shuman S.;
"Characterization of an ATP-dependent DNA ligase encoded by Haemophilus influenzae.";
Nucleic Acids Res. 25:1369-1374(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U17295; AAA95982.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L42023; AAC22836.1; ALT_FRAME; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L42023; AAC22846.1; ALT_FRAME; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D64021; D64021.
E64021; E64021.
3D structure databases
ModBase P44121.
Enzyme and pathway databases
BioCyc HINF71421:HI_1182-MON; -.
HINF71421:HI_1183-MON; -.
Family and domain databases
InterPro IPR012310; DNA_ligase_A_M.
IPR016059; DNA_ligase_CS.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.
Gene3D G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
Pfam PF01068; DNA_ligase_A_M; 1.
Pfam graphical view of domain structure.
PROSITE PS00697; DNA_LIGASE_A1; FALSE_NEG.
PS00333; DNA_LIGASE_A2; 1.
PS50160; DNA_LIGASE_A3; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P44121.
Genome annotation databases
TIGR HI1182; -.
HI1183; -.
Phylogenomic databases
HOGENOM P44121; -.
Other
ProtoNet P44121.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
CHAIN   1   268  268     DNA ligase. PRO_0000059625
ACT_SITE   41    41        N6-AMP-lysine intermediate (Potential). 
CONFLICT   93    93        T -> S (in Ref. 2; AAC22836). 
Sequence information
Length: 268 AA [This is the length of the unprocessed precursor] Molecular weight: 30896 Da [This is the MW of the unprocessed precursor] CRC64: F9EF9DD95B6B1252 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKFYRTLLLF FASSFAFANS DLMLLHTYNN QPIEGWVMSE KLDGVRGYWN GKQLLTRQGQ 

        70         80         90        100        110        120 
RLSPPAYFIK DFPPFAIDGE LFSERNHFEE ISTITKSFKG DGWEKLKLYV FDVPDAEGNL 

       130        140        150        160        170        180 
FERLAKLKAH LLEHPTTYIE IIEQIPVKDK THLYQFLAQV ENLQGEGVVV RNPNAPYERK 

       190        200        210        220        230        240 
RSSQILKLKT ARGEECTVIA HHKGKGQFEN VMGALTCKNH RGEFKIGSGF NLNERENPPP 

       250        260 
IGSVITYKYR GITNSGKPRF ATYWREKK 

P44121 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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NPSA logo NPSA Sequence analysis tools

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