RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Rinderpest virus (strain RBOK) (RDV)

[TaxID: 36409]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Morbillivirus.

Virus hosts

Bos indicus (Zebu) [TaxID: 9915]
Bos taurus (Bovine) [TaxID: 9913]
Bubalus bubalis (Domestic water buffalo) [TaxID: 89462]
Capra hircus (Goat) [TaxID: 9925]
Gazella (gazelles) [TaxID: 9933]
Giraffa camelopardalis (Giraffe) [TaxID: 9894]
Hippopotamus [TaxID: 9832]
Ovis aries (Sheep) [TaxID: 9940]
Suidae (pigs) [TaxID: 9821]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. PubMed=7897350 [NCBI, ExPASy, EBI, Israel, Japan] Baron M.D., Barrett T.; "Sequencing and analysis of the nucleocapsid (N) and polymerase (L) genes and the terminal extragenic domains of the vaccine strain of rinderpest virus."; J. Gen. Virol. 76:593-602(1995).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z30698; CAA83184.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Z30697; CAA83183.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S47307; S47307.

3D structure databases

ModBase

P41357.

Family and domain databases

InterPro

IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000830; RNA_pol_ParamyxoV; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2183`	`2183`	`Large structural protein.`	`PRO_0000142735`
`DOMAIN`	`656 840`	`185`	`RdRp catalytic.`
`NP_BIND`	`1785 1794`	`10`	`ATP (Potential).`

Sequence information

Length: 2183 AA [This is the length of the unprocessed precursor]

Molecular weight: 248110 Da [This is the MW of the unprocessed precursor]

CRC64: EF2C7D7E9E867F89 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDSLSVNQIL YPEVHLDSPI VTNKLVAILE YARIPHKYVL EDPTLCKNIK HRLDSGFSNQ 

        70         80         90        100        110        120 
MVINNVEIGN VVQSKLKGYP NHEHIPYPEC NQELFIARDK KATEKIRSLF KKGTTLYTKI 

       130        140        150        160        170        180 
SDKVCTCLET ANSRLGLGAD LGIGIKEKIL SLSLYMQSSQ WYEPFLFWFT IKTEMRSTIK 

       190        200        210        220        230        240 
SSVHTSHRRR YVPSFFSGDS FEILISRDLV AIVHRRLHHV YYLTFELVLM YCDIIEGRLM 

       250        260        270        280        290        300 
TETAMVIDPR YTELLERVRY MWKLIDGFFP TLGNSTYQVV ALLEPLSLAY LQLRDVTTEL 

       310        320        330        340        350        360 
RGAFLNHCFT EIREILNQNG VTDDNTYHEI VEALDYIFIT DDIHLTGEIF SFFRSFGHPR 

       370        380        390        400        410        420 
LEAITAADNV RKHMNQPKVI VYETLMKGHA IFCGIIINGY RDRHGGSWPP IHLPVHAATS 

       430        440        450        460        470        480 
IRNAQASGEG LTYEQCVDNW KSFAGIRFGC FMPLSLDSDL TMYLKDKALA ALKKEWDSAY 

       490        500        510        520        530        540 
PKEFLRYNPP KSTSSRRLVD VFLNDSTFDP YNMIMYVVSG DYLRDPDFNS SYSLKEKEIK 

       550        560        570        580        590        600 
ETGRLFAKMT YKMRACQVIA ENLISNGIGK YFRDNGMAKD EHDLTKALHT LAVSGVPKDF 

       610        620        630        640        650        660 
KDNYRGGPRA KTFSSKKTHT GAGISRVSRN EANYRSPEQR GCNTGGPDQI ESYETVSAFI 

       670        680        690        700        710        720 
TADLKKYCLN WRYETISLFA QRLNEIYGLP SFFQWLHRRL ERSVLYVSDP HCPPDLDSHA 

       730        740        750        760        770        780 
NLDNVPNDQI FIKYPMGGIE GYCQKLWTIS TIPYLYLAAH ESGVRIASLV QGDNQTIAVT 

       790        800        810        820        830        840 
KRVPSSWPYH LKKREAARVT REYFCLLRQR LHDIGHHLKA NETIVSSHFF VYSKGIYYDG 

       850        860        870        880        890        900 
LLISQSLKSI ARCVFWSETI VDETRAACSN IATTIAKSIE RGFDRYLAYS LNILKVIQQI 

       910        920        930        940        950        960 
LISLGFTINS TMTQDVVVPL LSNHDLLIRM ALLPAPIGGM NYLNMSRLFV RNIGDPVTSS 

       970        980        990       1000       1010       1020 
IADLKRMIGA SLMPEETLHQ VMTQQPGDSS FLDWASDPYS ANLVCIQSIT RLLKNITAKY 

      1030       1040       1050       1060       1070       1080 
VLINSPNPML RGLFHDDSKE EDEQLATFLM DRSVIVPRAA HEILDHSITG AREAIAGMLD 

      1090       1100       1110       1120       1130       1140 
TTKGLIRTSS RRGGLTTRVI ARLSTYDYEQ FRSGMVLLTG SKRNYLIDRD SCSVQLARAL 

      1150       1160       1170       1180       1190       1200 
RSHMWARLAR GRPIYGLEVP DVLESMRGHL IQRHATCILC DFGSANYGWF FVPSNCQLDD 

      1210       1220       1230       1240       1250       1260 
IDRETSALRV PYIGSTTDER TDMKLAFVKS PSQSLRSAVR IATVYSWAYG DDDKSWNEAL 

      1270       1280       1290       1300       1310       1320 
KLAQQRAKVS LEELKMITPI STSTNLAHRL RDRSTQVKYS GTSLVRVARY TTISNDNLSF 

      1330       1340       1350       1360       1370       1380 
VISDKKVDTN FIYQQGMLLG LGVLETLFRL DKDTGPSNTV LHLHVETDCC VIPMVDHPRI 

      1390       1400       1410       1420       1430       1440 
PSLHELKFRR ELCTNPLIYD SAPIIDREAT KLYTQSHRRH LVEFVTWSTS QLYHILAKST 

      1450       1460       1470       1480       1490       1500 
ALSMIDLVTK FEKDHMNEVS ALIGDDDINS FITEFLLVEP RLFTVYLGQC TAINWAFDIH 

      1510       1520       1530       1540       1550       1560 
YHRPSGKYQM GELLTSFLAR MSKSVFKVFV NALSHPKIYR KFWHSGIIEP IHGPSLDTQN 

      1570       1580       1590       1600       1610       1620 
LHITVCNMIY SCYMTYLDLL LNDELDEFTY LLCESDEDIV ADRFDNIQAR HLCVLSDLYC 

      1630       1640       1650       1660       1670       1680 
NPRGCPTIRG LQPVEKCAIM TKHIEAEAKL SPAGPSWNIG PIVIDHFSCS LTYLRRGSIK 

      1690       1700       1710       1720       1730       1740 
QIRLRVDPGF IFEALTVTDP QKPNFQHEQA ADMHISNFRP PYDGVAELLG TINSSKHNLP 

      1750       1760       1770       1780       1790       1800 
ILGTGVYNYE VHAFRRIGLN SSACYKAVEI STLIKSSMDP EEDSLFLGEG SGSMLITYKE 

      1810       1820       1830       1840       1850       1860 
ILKLKKCFYN SGVSAESRSG QRELAPYPSE VSLVEHQLGA EKTVKVLFNG RPEVTWIGSV 

      1870       1880       1890       1900       1910       1920 
DCFNYIISNI QTSSLGLIHS DIETLPTKDI TEKLEELSAI LALALLLGKV GSVLVIKVMP 

      1930       1940       1950       1960       1970       1980 
ISGDYVQGFM SYMVPYYREV LIIYPRYSNF ISTEAYLVLL GLRANRLIDP ERIKQQVMES 

      1990       2000       2010       2020       2030       2040 
GIRTIPGLVG HILSIKQLNC IQSQAGPAIT RGEINPILRK LTSIERILIS CGLTINGTKI 

      2050       2060       2070       2080       2090       2100 
CSDVVHHDIS SGPDGLLNSS IILLQELARF KDNQRSQQGM FHAYPVLLSS RQRELISRVA 

      2110       2120       2130       2140       2150       2160 
RKFWGYILLY SSDRRLLSRL VSNLKSGYLL FDLHQNLFMK NLSKSEKQLI RTGGLKREWL 

      2170       2180 
FKLTTKEIKE WFKLIGYSAL IRE

P41357 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools