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UniProtKB/Swiss-Prot entry P39683


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NPT1_YEAST
Primary accession number P39683
Secondary accession number Q08626
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on November 1, 1997 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 80)
Name and origin of the protein
Protein name Nicotinate phosphoribosyltransferase
Synonyms NAPRTase
EC 2.4.2.11
Gene name
Name: NPT1
OrderedLocusNames: YOR209C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 28383 / FL100 / VTT C-80102;
Lalo D., Doira C., Thuriaux P.;
"The BRF1-STE4 region chromosome XV contains eleven genes: two of them define distinct RNA polymerase subunits and one encodes a putative nicotinate phosphorybosyl transferase.";
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169874 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
Nature 387:98-102(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-43.
STRAIN=ATCC 28383 / FL100 / VTT C-80102;
PubMed=8516295 [NCBI, ExPASy, EBI, Israel, Japan]
Lalo D., Carles C., Sentenac A., Thuriaux P.;
"Interactions between three common subunits of yeast RNA polymerases I and III.";
Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993).
[4]
FUNCTION.
DOI=10.1016/S0014-5793(02)02585-1; PubMed=12062417 [NCBI, ExPASy, EBI, Israel, Japan]
Panozzo C., Nawara M., Suski C., Kucharczyka R., Skoneczny M., Becam A.-M., Rytka J., Herbert C.J.;
"Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae.";
FEBS Lett. 517:97-102(2002).
[5]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02026; PubMed=14562095 [NCBI, ExPASy, EBI, Israel, Japan]
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.;
"Global analysis of protein localization in budding yeast.";
Nature 425:686-691(2003).
[6]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z36878; CAA85352.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z75117; CAA99424.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L11274; AAB59317.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S67101; S67101.
RefSeq NP_014852.1; -.
3D structure databases
PDB
1VLP; X-ray; 1.75 A; A/B/C/D=1-429.[ExPASy / RCSB / EBI]
PDBsum 1VLP; -.
ModBase P39683.
Protein-protein interaction databases
DIP DIP:4984N; -.
IntAct P39683; -.
Organism-specific databases
CYGD YOR209c; -.
SGD S000005735; NPT1.
Yeast-GFP YOR209C.
Gene expression databases
ArrayExpress P39683; -.
GermOnline YOR209C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from direct assay from SGD).
GO:0004516; Molecular function: nicotinate phosphoribosyltransferase activity (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0000183; Biological process: chromatin silencing at rDNA (inferred from mutant phenotype from SGD).
GO:0006348; Biological process: chromatin silencing at telomere (inferred from mutant phenotype from SGD).
GO:0019358; Biological process: nicotinate nucleotide salvage (inferred from direct assay from SGD).
GO:0001302; Biological process: replicative cell aging (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR006406; Nic_PRibTrfase.
IPR015977; Nic_PRibTrfase-like.
IPR007229; Nic_PRibTrfase-rel.
Graphical view of domain structure.
PANTHER PTHR11098:SF1; NAPRTase; 1.
Pfam PF04095; NAPRTase; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000484; NAPRT; 1.
TIGRFAMs TIGR01514; NAPRTase; 1.
BLOCKS P39683.
Proteomic databases
PeptideAtlas P39683; -.
Genome annotation databases
Ensembl YOR209C; Saccharomyces cerevisiae. [Contig view]
GeneID 854384; -.
GenomeReviews Y13140_GR; YOR209C.
KEGG sce:YOR209C; -.
NMPDR fig|4932.3.peg.5964; -.
Phylogenomic databases
HOGENOM P39683; -.
Other
LinkHub P39683; -.
ProtoNet P39683.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Cytoplasm; Glycosyltransferase; Nucleus; Pyridine nucleotide biosynthesis; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   429  429     Nicotinate phosphoribosyltransferase. PRO_0000205862
CONFLICT   131   137        YEIPLLS -> MRSLTV (in Ref. 1; CAA85352). 
CONFLICT   144   158        FKFVDIDWDYENQLE -> LIVTSTGLRNHR (in Ref. 1). 
CONFLICT   175   177        SEF -> RIH (in Ref. 1; CAA85352). 
CONFLICT   243   243        E -> EL (in Ref. 1; CAA85352). 
STRAND   11    13  3      
HELIX   14    26  13      
STRAND   31    39  9      
HELIX   47    59  13      
HELIX   60    62  3      
HELIX   67    76  10      
HELIX   82    89  8      
HELIX   97   100  4      
STRAND   101   108  8      
STRAND   111   124  14      
HELIX   125   128  4      
HELIX   132   146  15      
HELIX   156   169  14      
STRAND   174   176  3      
HELIX   185   201  17      
HELIX   203   206  4      
TURN   207   209  3      
STRAND   210   215  6      
HELIX   216   222  7      
HELIX   232   242  11      
HELIX   248   260  13      
HELIX   262   264  3      
STRAND   267   269  3      
HELIX   275   279  5      
HELIX   286   289  4      
STRAND   292   295  4      
HELIX   300   312  13      
STRAND   321   326  6      
HELIX   332   344  13      
STRAND   348   353  6      
HELIX   355   358  4      
STRAND   364   366  3      
STRAND   369   371  3      
STRAND   376   383  8      
HELIX   402   411  10      
Sequence information
Length: 429 AA [This is the length of the unprocessed precursor] Molecular weight: 49019 Da [This is the MW of the unprocessed precursor] CRC64: F3F7941476C3398B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSEPVIKSLL DTDMYKITMH AAVFTNFPDV TVTYKYTNRS SQLTFNKEAI NWLKEQFSYL 

        70         80         90        100        110        120 
GNLRFTEEEI EYLKQEIPYL PSAYIKYISS SNYKLHPEEQ ISFTSEEIEG KPTHYKLKIL 

       130        140        150        160        170        180 
VSGSWKDTIL YEIPLLSLIS EAYFKFVDID WDYENQLEQA EKKAETLFDN GIRFSEFGTR 

       190        200        210        220        230        240 
RRRSLKAQDL IMQGIMKAVN GNPDRNKSLL LGTSNILFAK KYGVKPIGTV AHEWVMGVAS 

       250        260        270        280        290        300 
ISEDYLHANK NAMDCWINTF GAKNAGLALT DTFGTDDFLK SFRPPYSDAY VGVRQDSGDP 

       310        320        330        340        350        360 
VEYTKKISHH YHDVLKLPKF SKIICYSDSL NVEKAITYSH AAKENGMLAT FGIGTNFTND 

       370        380        390        400        410        420 
FRKKSEPQVK SEPLNIVIKL LEVNGNHAIK ISDNLGKNMG DPATVKRVKE ELGYTERSWS 


GDNEAHRWT 

P39683 in FASTA format

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